Tomoko Okada
YOU?
Author Swipe
View article: Photoinduced Actin Aggregation Involves Cell Death: A Mechanism of Cancer Cell Cytotoxicity after Near-Infrared Photoimmunotherapy
Photoinduced Actin Aggregation Involves Cell Death: A Mechanism of Cancer Cell Cytotoxicity after Near-Infrared Photoimmunotherapy Open
Near-infrared photoimmunotherapy (NIR-PIT) is a cancer treatment modality that uses antibody-photoabsorber (IR700) conjugates to destroy specific cells. The reaction between the antibody and photoabsorber is triggered by NIR-light, and thi…
View article: Direct Observation of Biological Fine Particles in Water by Scanning Electron Assisted Dielectric Microscopy
Direct Observation of Biological Fine Particles in Water by Scanning Electron Assisted Dielectric Microscopy Open
Investigation of the effects of nanoparticles and extracellular vesicles on cells requires direct observation and analysis at nano-level resolution, both in vitro and in living cells. Recently, we have developed a scanning electron assiste…
View article: Observation of biological and emulsion samples by newly developed three-dimensional impedance scanning electron microscopy
Observation of biological and emulsion samples by newly developed three-dimensional impedance scanning electron microscopy Open
Imaging at nanometre-scale resolution is indispensable for many scientific fields such as biology, chemistry, material science and nanotechnology. Scanning electron microscopes (SEM) are widely used as important tools for the nanometre-sca…
View article: Direct observation of the effects of chemical fixation in MNT-1 cells: A SE-ADM and Raman study
Direct observation of the effects of chemical fixation in MNT-1 cells: A SE-ADM and Raman study Open
Aldehydes fixation was accidentally discovered in the early 20th century and soon became a widely adopted practice in the histological field, due to an excellent staining enhancement in tissues imaging. However, the fixation process itself…
View article: Development of General-purpose Dielectric Constant Imaging Unit for SEM and Direct Observation of Samples in Aqueous Solution
Development of General-purpose Dielectric Constant Imaging Unit for SEM and Direct Observation of Samples in Aqueous Solution Open
Electron microscopes can observe samples with a spatial resolution of 10 nm or higher; however, they cannot observe samples in solutions due to the vacuum conditions inside the sample chamber. Recently, we developed a scanning electron-ass…
View article: Preferential Selection of Low-Frequency, Lipopolysaccharide-Modified, Colistin-Resistant Mutants with a Combination of Antimicrobials in Acinetobacter baumannii
Preferential Selection of Low-Frequency, Lipopolysaccharide-Modified, Colistin-Resistant Mutants with a Combination of Antimicrobials in Acinetobacter baumannii Open
Acinetobacter baumannii has developed resistance to various antimicrobial drugs, and its drug-resistant strains cause nosocomial infections. Controlling these infections has become a global clinical challenge.
View article: Unique Glycoform-Dependent Monoclonal Antibodies for Mouse Mucin 21
Unique Glycoform-Dependent Monoclonal Antibodies for Mouse Mucin 21 Open
Mucin 21(Muc21)/epiglycanin is expressed on apical surfaces of squamous epithelia and has potentially protective roles, which are thought to be associated with its unique glycoforms, whereas its aberrant glycosylation is implicated in the …
View article: Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells
Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells Open
Autophagy is an intracellular self-devouring system that plays a central role in cellular recycling. The formation of functional autophagosomes depends on several autophagy-related proteins, including the microtubule-associated proteins 1A…
View article: Histamine H1 receptor antagonists selectively kill cisplatin-resistant human cancer cells
Histamine H1 receptor antagonists selectively kill cisplatin-resistant human cancer cells Open
Cancer therapy is often hampered by the disease’s development of resistance to anticancer drugs. We previously showed that the autonomously upregulated product of fibroblast growth factor 13 gene ( FGF13 ; also known as FGF homologous fact…
View article: High-resolution imaging of autophagosome formation by LC3 and ATG12 in cells using scanning electron-assisted dielectric microscopy
High-resolution imaging of autophagosome formation by LC3 and ATG12 in cells using scanning electron-assisted dielectric microscopy Open
Background Autophagy is an intracellular self-eating system that plays a central role in cellular recycling. The formation of functional autophagosomes depends on several autophagy- related proteins, including LC3 and Atg12. To analyse an …
View article: Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro
Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro Open
The contribution of secretions from tumor-associated macrophage (TAM)-like cells to the stimulation of mechanical property changes in murine breast cancer cells was studied using an in vitro model system. A murine breast cancer cell line (…
View article: Osteoblastic lysosome plays a central role in mineralization
Osteoblastic lysosome plays a central role in mineralization Open
Lysosome transports mineralizing nanovesicle in osteoblasts.
View article: The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis
The Structural Function of Nestin in Cell Body Softening is Correlated with Cancer Cell Metastasis Open
Intermediate filaments play significant roles in governing cell stiffness and invasive ability. Nestin is a type VI intermediate filament protein that is highly expressed in several high-metastatic cancer cells. Although inhibition of nest…
View article: Nanoscale imaging of the adhesion core including integrin β1 on intact living cells using scanning electron-assisted dielectric-impedance microscopy
Nanoscale imaging of the adhesion core including integrin β1 on intact living cells using scanning electron-assisted dielectric-impedance microscopy Open
The integrins are a superfamily of transmembrane proteins composed of α and β subunit dimers involved in cell-cell and cell-extracellular matrix interactions. The largest integrin subgroup is integrin β1, which contributes to several malig…
View article: Nanoscale observation of the natural structure of milk-fat globules and casein micelles in the liquid condition using a scanning electron assisted dielectric microscopy
Nanoscale observation of the natural structure of milk-fat globules and casein micelles in the liquid condition using a scanning electron assisted dielectric microscopy Open
Recently, aqueous nanoparticles have been used in drug-delivery systems for new type medicines. In particular, milk-casein micelles have been used as drug nanocarriers for targeting cancer cells. Therefore, nanostructure observation of par…
View article: Nanoscale Observation of Intact Biological Specimens in Water with High-contrast Imaging by Scanning Electron Assisted Dielectric-impedance Microscopy
Nanoscale Observation of Intact Biological Specimens in Water with High-contrast Imaging by Scanning Electron Assisted Dielectric-impedance Microscopy Open
Journal Article Nanoscale Observation of Intact Biological Specimens in Water with High-contrast Imaging by Scanning Electron Assisted Dielectric-impedance Microscopy Get access T Ogura, T Ogura Biomedical Research Institute, National Inst…
View article: Morphology-based optical separation of subpopulations from a heterogeneous murine breast cancer cell line
Morphology-based optical separation of subpopulations from a heterogeneous murine breast cancer cell line Open
Understanding tumor heterogeneity is an urgent and unmet need in cancer research. In this study, we used a morphology-based optical cell separation process to classify a heterogeneous cancer cell population into characteristic subpopulatio…
View article: High-resolution imaging of living mammalian cells bound by nanobeads-connected antibodies in a medium using scanning electron-assisted dielectric microscopy
High-resolution imaging of living mammalian cells bound by nanobeads-connected antibodies in a medium using scanning electron-assisted dielectric microscopy Open
Nanometre-scale-resolution imaging technologies for liquid-phase specimens are indispensable tools in various scientific fields. In biology, observing untreated living cells in a medium is essential for analysing cellular functions. Howeve…
View article: Expression of Cadherin-17 Promotes Metastasis in a Highly Bone Marrow Metastatic Murine Breast Cancer Model
Expression of Cadherin-17 Promotes Metastasis in a Highly Bone Marrow Metastatic Murine Breast Cancer Model Open
We previously established 4T1E/M3 highly bone marrow metastatic mouse breast cancer cells through in vivo selection of 4T1 cells. But while the incidence of bone marrow metastasis of 4T1E/M3 cells was high (~80%) when injected intravenousl…
View article: Improvement of intratumor microdistribution of PEGylated liposome via tumor priming by metronomic S-1 dosing
Improvement of intratumor microdistribution of PEGylated liposome via tumor priming by metronomic S-1 dosing Open
The efficient delivery of nanocarrier-based cancer therapeutics into tumor tissue is problematic. Structural abnormalities, tumor vasculature heterogeneity, and elevated intratumor pressure impose barriers against the preferential accumula…