Toshihiko Toida
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View article: Rapid Purification of Salmon Nasal Cartilage Proteoglycan by High‐Salt Ethanol Precipitation
Rapid Purification of Salmon Nasal Cartilage Proteoglycan by High‐Salt Ethanol Precipitation Open
Proteoglycan (PG) from salmon nasal cartilage has received increasing attention owing to its potential health benefits such as improving joint function and muscle strength. However, conventional purification methods involve multiple fracti…
View article: Polyamines stimulate the protein synthesis of the translation initiation factor eIF5A2, participating in mRNA decoding, distinct from eIF5A1
Polyamines stimulate the protein synthesis of the translation initiation factor eIF5A2, participating in mRNA decoding, distinct from eIF5A1 Open
Polyamines are present in all living organisms, and their homeostasis is closely associated with human health and disease. Furthermore, they are small aliphatic cations that exhibit multifunctional activities through interactions with acid…
View article: Analysis of Acetylated Hyaluronic Acid in Moisturizing Lotion and Milk Lotion by HPLC with Fluorescence Detection
Analysis of Acetylated Hyaluronic Acid in Moisturizing Lotion and Milk Lotion by HPLC with Fluorescence Detection Open
We developed a simple and sensitive analytical HPLC method for the determination of acetylated hyaluronic acid (AcHA) in moisturizing and milk lotions. AcHA with different molecular weights was separated as a single peak using a C4 column …
View article: Fucosylated heparan sulfate from the midgut gland of Patinopecten yessoensis
Fucosylated heparan sulfate from the midgut gland of Patinopecten yessoensis Open
The structural and functional relationships of glycosaminoglycans (GAGs) derived from marine organisms have been investigated, suggesting that marine invertebrates, particularly Bivalvia, are abundant sources of highly sulfated or branched…
View article: Simultaneous determination of intracellular reduced and oxidized glutathiones by the König reaction
Simultaneous determination of intracellular reduced and oxidized glutathiones by the König reaction Open
The König reaction is newly applied to the simultaneous determination of reduced and oxidized glutathione.
View article: Characterization of chondroitin sulfate in stem cells derived from umbilical cord blood in rats
Characterization of chondroitin sulfate in stem cells derived from umbilical cord blood in rats Open
Chondroitin sulfate (CS) and its isomeric variant, dermatan sulfate (DS), are complex glycosaminoglycans (GAGs) which are ubiquitous components of the extracellular matrix in various tissues including the brain. CS and/or DS are known to b…
View article: Structure and Immunomodulatory Activity of Glycogen Derived from Honeybee Larvae (<i>Apis mellifera</i>)
Structure and Immunomodulatory Activity of Glycogen Derived from Honeybee Larvae (<i>Apis mellifera</i>) Open
Honeybee larvae have been recognized as nutrient-rich food in many countries. Although glycogen, a storage form of glucose in animals, is synthesized in honeybee larvae, there is no information on the structure of glycan and its biological…
View article: KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation
KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation Open
Mast cells have secretory granules containing chemical mediators such as histamine and play important roles in the immune system. Polyamines are essential factors for cellular processes such as gene expression and translation. It has been …
View article: Detection of Selenocyanate in Biological Samples by HPLC with Fluorescence Detection Using König Reaction
Detection of Selenocyanate in Biological Samples by HPLC with Fluorescence Detection Using König Reaction Open
We developed a simple and sensitive HPLC method for the determination of selenocyanate (SeCN-). The König reaction, which is generally used for the determination of cyanide and thiocyanate, was applied for the post-column detection, and us…
View article: Preparation of <sup>99m</sup>Tc-Labeled Mannan-<i>S</i>-Cysteine and Effect of Molecular Size of Mannan on Its Biodistribution
Preparation of <sup>99m</sup>Tc-Labeled Mannan-<i>S</i>-Cysteine and Effect of Molecular Size of Mannan on Its Biodistribution Open
Macrophage mannose receptor (MMR/CD206) is a promising target for the detection and identification of sentinel lymph node (SLN). MMR-targeting probes have been developed using mannosylated dextran, however, impairment of efficient targetin…
View article: Identification of Chondroitin Sulfates Having Unique Structures from Shellfish Using Chondroitinase ACII
Identification of Chondroitin Sulfates Having Unique Structures from Shellfish Using Chondroitinase ACII Open
海洋生物、特に二枚貝は抗凝固薬ヘパリンの代替資源として知られている。コンドロイチン硫酸（CS）もヘパリンと同様に二枚貝から抽出されるが、CSの詳細な構造解析は行われなかった。近年、私達は11種の食用貝から抽出したCSをコンドロイチナーゼABC又はACIIで処理し、得られた不飽和二糖をHPLCで分析した。その結果、いくつかの貝類で、未同定ピークがコンドロイチナーゼABCではなくコンドロイチナーゼACII処理により検出された。バカガイのCSでは、グルクロン酸のC-3位にケラタン…
View article: Identification of Chondroitin Sulfates Having Unique Structures from Shellfish Using Chondroitinase ACII
Identification of Chondroitin Sulfates Having Unique Structures from Shellfish Using Chondroitinase ACII Open
Marine organisms, especially bivalve molluscs have been known as an alternative source of heparin, an anticoagulant. Although chondroitin sulfate (CS) can be also extracted together with heparin, the detailed glycan structures of CS have n…
View article: Protective Effects of Brain Infarction by<i>N</i>-Acetylcysteine Derivatives
Protective Effects of Brain Infarction by<i>N</i>-Acetylcysteine Derivatives Open
Background and Purpose— We recently found that acrolein (CH 2 =CH-CHO) is more strongly involved in brain infarction compared with reactive oxygen species. In this study, we looked for acrolein scavengers with less side effects. Methods— P…
View article: Evaluation of Immunological and Moisturizing Activities of beta-glucan Isolated from Molasses Yeast Waste
Evaluation of Immunological and Moisturizing Activities of beta-glucan Isolated from Molasses Yeast Waste Open
Systematic extraction and purification of β-glucan was attempted from molasses yeast (Saccharomyces cerevisiae) waste available from ethanol industry. The aim was to create value for the waste material by making it suitable for topical use…
View article: Sequencing the Dermatan Sulfate Chain of Decorin
Sequencing the Dermatan Sulfate Chain of Decorin Open
Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable based on proteomic o…
View article: Cloning and Expression of Recombinant Chondroitinase ACII and Its Comparison to the <i>Arthrobacter aurescens</i> Enzyme
Cloning and Expression of Recombinant Chondroitinase ACII and Its Comparison to the <i>Arthrobacter aurescens</i> Enzyme Open
Chondroitin sulfates are the glycosaminoglycan chains of proteoglycans critical in the normal development and pathophysiology of all animals. Chondroitinase ACII, a polysaccharide lyase originally isolated from Arthrobacter aurescens IAM 1…
View article: Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor‐2 signaling impairment in the aged neurogenic niche
Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor‐2 signaling impairment in the aged neurogenic niche Open
Adult neurogenesis in the subventricular zone of the lateral ventricle decreases with age. In the subventricular zone, the specialized extracellular matrix structures, known as fractones, contact neural stem cells and regulate neurogenesis…
View article: [Regular Paper] Disaccharide Composition of Chondroitin Sulfate Derived from Lateolabrax japonicas
[Regular Paper] Disaccharide Composition of Chondroitin Sulfate Derived from Lateolabrax japonicas Open
コンドロイチン硫酸(CS) はグルクロン酸(GlcA) とN-アセチルガラクトサミン(GalNAc) が交互に結合し,各糖ヒドロキシ基の一部に硫酸基が付加した直鎖の酸性多糖類である.GlcA の一部がイズロン酸(IdoA) に変換されたCS 鎖はデルマタン硫酸(DS) と呼ばれる.CS およびDS はA-unit (GlcA/IdoA-Gal-NAc4S),B-unit (IdoA2S-GalNAc4S),C-unit (GlcA/IdoA-GalNAc6S),D-unit…
View article: Preparation of the Partially Methylated Alditol Acetates Derived from CS Tetrasaccharides Containing Galactose for the Gas Chromatography/Mass Spectrometry Analysis
Preparation of the Partially Methylated Alditol Acetates Derived from CS Tetrasaccharides Containing Galactose for the Gas Chromatography/Mass Spectrometry Analysis Open
Chondroitin sulfate (CS), a member of the glycosaminoglycan (GAG) family of carbohydrates, is composed of linear, sulfated repeating disaccharide sequences of N-acetyl-D-galactosamine (GalNAc) and glucuronic acid (GlcA). Recently, a kerata…
View article: Isolation of Keratan Sulfate Disaccharide-branched Chondroitin Sulfate E from Mactra chinensis
Isolation of Keratan Sulfate Disaccharide-branched Chondroitin Sulfate E from Mactra chinensis Open
Glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), heparin (HP), heparan sulfate (HS) and keratan sulfate (KS) are linear, sulfated repeating disaccharide sequences containing hexosamine and uronic acid (o…
View article: Polyamines release the let-7b-mediated suppression of initiation codon recognition during the protein synthesis of EXT2
Polyamines release the let-7b-mediated suppression of initiation codon recognition during the protein synthesis of EXT2 Open
Proteoglycans (PGs), a family of glycosaminoglycan (GAG)-protein glycoconjugates, contribute to animal physiology through interactions between their glycan chains and growth factors, chemokines and adhesion molecules. However, it remains u…
View article: Identification of keratan sulfate disaccharide at C-3 position of glucuronate of chondroitin sulfate from <i>Mactra chinensis</i>
Identification of keratan sulfate disaccharide at C-3 position of glucuronate of chondroitin sulfate from <i>Mactra chinensis</i> Open
Glycosaminoglycans (GAGs), including chondroitin sulfate (CS), dermatan sulfate, heparin, heparan sulfate and keratan sulfate (KS) are linear sulfated repeating disaccharide sequences containing hexosamine and uronic acid [or galactose (Ga…
View article: Recent Progress in Analytical Methods for Determination of Urinary 3-Hydroxypropylmercapturic Acid, a Major Metabolite of Acrolein
Recent Progress in Analytical Methods for Determination of Urinary 3-Hydroxypropylmercapturic Acid, a Major Metabolite of Acrolein Open
3-Hydroxypropylmercapturic acid (3-HPMA), a major metabolite of acrolein in urine, has been recognized as a noninvasive biomarker of exposure to cigarette smoke. Since acrolein is formed endogenously from polyamines and is also formed duri…
View article: Poly-ion Complex of Chondroitin Sulfate and Spermine and Its Effect on Oral Chondroitin Sulfate Bioavailability
Poly-ion Complex of Chondroitin Sulfate and Spermine and Its Effect on Oral Chondroitin Sulfate Bioavailability Open
Chondroitin sulfate (CS) has been accepted as an ingredient in health foods for the treatment of symptoms related to arthritis and cartilage repair. However, CS is poorly absorbed through the gastrointestinal tract because of its high nega…