Troy Taylor
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View article: Producing recombinant proteins in Vibrio natriegens
Producing recombinant proteins in Vibrio natriegens Open
The diversity of chemical and structural attributes of proteins makes it inherently difficult to produce a wide range of proteins in a single recombinant protein production system. The nature of the target proteins themselves, along with c…
View article: Producing recombinant proteins in Vibrio natriegens
Producing recombinant proteins in Vibrio natriegens Open
The diversity of chemical and structural attributes of proteins makes it inherently difficult to produce a wide range of proteins in a single recombinant protein production system. The nature of the target proteins themselves, along with c…
View article: Comparative analysis of KRAS4a and KRAS4b splice variants reveals distinctive structural and functional properties
Comparative analysis of KRAS4a and KRAS4b splice variants reveals distinctive structural and functional properties Open
KRAS , the most frequently mutated oncogene in human cancer, produces two isoforms, KRAS4a and KRAS4b, through alternative splicing. These isoforms differ in exon 4, which encodes the final 15 residues of the G-domain and hypervariable reg…
View article: A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike
A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike Open
Neutralizing antibodies targeting the SARS-CoV-2 spike protein have shown a great preventative/therapeutic potential. Here, we report a rapid and efficient strategy for the development and design of SARS-CoV-2 neutralizing humanized nanobo…
View article: Insights into the Cross Talk between Effector and Allosteric Lobes of KRAS from Methyl Conformational Dynamics
Insights into the Cross Talk between Effector and Allosteric Lobes of KRAS from Methyl Conformational Dynamics Open
KRAS is the most frequently mutated RAS protein in cancer patients, and it is estimated that about 20% of the cancer patients in the United States carried mutant RAS proteins. To accelerate therapeutic development, structures and dynamics …
View article: A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike
A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike Open
Neutralizing antibodies targeting the SARS-CoV-2 spike protein have shown a great preventative/therapeutic potential. Here, we report a rapid and efficient strategy for the development and design of SARS-CoV-2 neutralizing humanized nanobo…
View article: Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays
Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays Open
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a commonly used antigen for serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Different versions of the RBD protein have been d…
View article: Uncovering a membrane-distal conformation of KRAS available to recruit RAF to the plasma membrane
Uncovering a membrane-distal conformation of KRAS available to recruit RAF to the plasma membrane Open
Significance The proto-oncogene KRAS , a small GTPase, is frequently mutated in pancreatic, colorectal, and lung cancer. These mutations result in elevated levels of the activated guanosine triphosphate-bound form of KRAS. Localized at the…
View article: KRAS interaction with RAF1 RAS-binding domain and cysteine-rich domain provides insights into RAS-mediated RAF activation
KRAS interaction with RAF1 RAS-binding domain and cysteine-rich domain provides insights into RAS-mediated RAF activation Open
A vital first step of RAF activation involves binding to active RAS, resulting in the recruitment of RAF to the plasma membrane. To understand the molecular details of RAS-RAF interaction, we solved crystal structures of wild-type and onco…
View article: Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays Open
The SARS-CoV-2 spike trimer is the primary antigen for several serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Until stable cell lines are developed to increase the titer of this secreted protei…
View article: PDE6D Inhibitors with a New Design Principle Selectively Block K-Ras Activity
PDE6D Inhibitors with a New Design Principle Selectively Block K-Ras Activity Open
The trafficking chaperone PDE6D (also referred to as PDEδ) has been nominated as a surrogate target for K-Ras4B (hereafter K-Ras). Arl2-assisted unloading of K-Ras from PDE6D in the perinuclear area is significant for correct K-Ras localiz…
View article: KRAS Prenylation Is Required for Bivalent Binding with Calmodulin in a Nucleotide-Independent Manner
KRAS Prenylation Is Required for Bivalent Binding with Calmodulin in a Nucleotide-Independent Manner Open
Deregulation of KRAS4b signaling pathway has been implicated in 30% of all cancers. Membrane localization of KRAS4b is an essential step for the initiation of the downstream signaling cascades that guide various cellular mechanisms. KRAS4b…