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View article: Macropinocytosis enables metabolic recycling of extracellular DNA in cancer cells
Macropinocytosis enables metabolic recycling of extracellular DNA in cancer cells Open
Avid nutrient consumption is a metabolic hallmark of cancer and leads to regional depletion of key metabolites within the tumor microenvironment (TME). Cancer cells consequently employ diverse strategies to acquire the fuels needed for gro…
View article: Longitudinal tracking of MALAT1 level over a breast cancer patient’s course of treatment and disease progression
Longitudinal tracking of MALAT1 level over a breast cancer patient’s course of treatment and disease progression Open
Long non-coding RNAs (lncRNAs) have emerged as important regulators of the hallmarks of cancer, yet their potential remains under-explored in clinical studies. Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1), …
View article: Supplementary Table S10A from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S10A from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
RNA-seq results. Combined tables for DESeq2 output of Ctrl, MARK2/3, YAP/TAZ double knockout in 20 cancer cell lines.
View article: Supplementary Table S5 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S5 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
P value for synergy score paralog combinations. Table of P value corresponding to synergy scores calculated using GEMINI.
View article: Supplementary Figure S7 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S7 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A, IP–western blot analysis evaluating the interaction between 14-3-3e and YAPWT and YAP5D mutants or TAZWT and TAZ4D mutants in HEK-293T cells. Aspartate mutant reversion to wild-type S/T is indicated. Data are representative of two indep…
View article: Supplementary Table S10B from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S10B from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
RNA-seq results. Combined tables for DESeq2 output of Ctrl, MARK2/3, YAP/TAZ double knockout in 20 cancer cell lines.
View article: Supplementary Table S2 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S2 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
Paralog CRISPR screening data 2. Table of calculated gene level log2(fold-changes) of Paralog
View article: Supplementary Table S3 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S3 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
Cancer cell line model information. Table of mutations and cancer driver of cell lines used in this study.
View article: Data from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Data from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
The Hippo signaling pathway is commonly dysregulated in human cancer, which leads to a powerful tumor dependency on the YAP/TAZ transcriptional coactivators. In this study, we used paralog cotargeting CRISPR screens to identify kinases MAR…
View article: Supplementary Figure S10 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S10 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A, Western blot analysis of YAP localization following doxycycline (Dox) induced empty vector control, MKIWT or MKIMUT expression for 24h in YAPC cells. Nuclear (Nuc) and cytosolic (Cyto) fractionation are indicated. (Data shown are an ext…
View article: Supplementary Table S1 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S1 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
Paralog CRISPR screening data. Table for sgRNA sequences and raw sequencing counts of paralog CRISPR screens in 22 cancer cell lines.
View article: Supplementary Table S6 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S6 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
FDR for synergy score paralog combinations. Table of FDR corresponding to synergy scores calculated using GEMINI.
View article: Supplementary Table S4 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S4 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
Synergy score paralog combinations. Table of synergy scores calculated using GEMINI.
View article: Supplementary Table S9 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S9 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
sgRNA sequences. Table of all sgRNA-sequences used in this study.
View article: Supplementary Figure S9 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S9 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A, Sanger sequencing Chromatogram of NF2 locus in MDA-MB231 cells. B, C, Rescue experiment of MARK2+3dKO in Cas9+ YAPC cells following knockout of indicated genes. Data shown are the mean ± SD of %GFP+ (normalized to day 3 after infection)…
View article: Supplementary Table S8 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S8 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
Phosphosite identification using mass spectrometry. Combined table of peptides identified using mass spectrometry.
View article: Supplementary Figure S8 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S8 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A, Rescue experiment of MARK2+3dKO in Cas9+ YAPC cells following knockout of indicated genes. Data shown are the mean ± SD of %GFP+ (normalized to day 3 after infection). n=3-6. P values are calculated using a mixed effects model (consider…
View article: Supplementary Table S11 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S11 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
YAP:TEAD4 bound YAP/TAZ dependent enhancers. Peak location file for enhancers with YAP,TEAD4 binding, and reduction of signal upon YAP/TAZ knockout.
View article: Supplementary Figure S1 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S1 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A,B, CRISPR screening results from 22 cancer cell lines. A, Abundance fold-change of positive controls (dgRNAs targeting essential genes n=28 paired with control) and negative controls (dgRNAs targeting non-coding regions n=54 and nontarge…
View article: Supplementary Figure S4 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S4 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A, Western blot analysis of Cas9+ YAPC cells. B, Representative immunofluorescence (IF) images of YAP and DAPI following MARK2+3dKO in A549 cancer cells. scale bar = 30 µm. C, Comparison of mean immunofluorescence signal of YAP across the …
View article: Supplementary Data MS from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Data MS from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
Mass spectrometric identification and quantification of MARK2 substrate peptides This document contains mass spectrometric data supporting phosphorylation of specific phosphorylation sites on CDC25C, YAP, TAZ, and NF2. For each considered …
View article: Supplementary Figure S3 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S3 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A, Mutational two-class comparison between 19 MARK2/3-dependent and 12 MARK2/3-independent human cancer cell lines (shown in B). Mutation data were obtained from the CCLE database. The effect size and P values were calculated for every mut…
View article: Supplementary Figure S2 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S2 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A, Growth kinetics of subcutaneous YAPC xenografts implanted in immunodeficient mice. Indicated genes were knocked out just before injection. Data are shown as mean ± SEM n=5 per group. P values are calculated using a mixed effects model (…
View article: Supplementary Table S7 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Table S7 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
YAP/TAZ signature. Table of genes used as YAP/TAZ signature.
View article: Supplementary Figure S6 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S6 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A-E, Western blot analysis of MARK2 specific substrates phosphorylation. Labeling as described in Fig 3D. Data are representative of two independent experiments. F, Lolli-pop illustration of MARK2-dependent phosphorylation sites on MST1/2 …
View article: Supplementary Figure S5 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
Supplementary Figure S5 from MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
A-K, Fragmentation spectrum supporting peptide identity and phosphosites on YAP, TAZ, and NF2. Precursor ion chromatogram (XIC) and corresponding ions are provided in supplementary materials.
View article: PTPN23-dependent ESCRT machinery functions as a cell death checkpoint
PTPN23-dependent ESCRT machinery functions as a cell death checkpoint Open
View article: A brain-body feedback loop driving HPA-axis dysfunction in breast cancer
A brain-body feedback loop driving HPA-axis dysfunction in breast cancer Open
Breast cancer patients often exhibit disrupted circadian rhythms in circulating glucocorticoids (GCs), such as cortisol. This disruption correlates with reduced quality of life and higher cancer mortality 1–3 . The exact cause of this phen…
View article: MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer
MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer Open
The Hippo signaling pathway is commonly dysregulated in human cancer, which leads to a powerful tumor dependency on the YAP/TAZ transcriptional coactivators. In this study, we used paralog cotargeting CRISPR screens to identify kinases MAR…
View article: SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23
SRSF1 interactome determined by proximity labeling reveals direct interaction with spliceosomal RNA helicase DDX23 Open
SRSF1 is the founding member of the SR protein family. It is required—interchangeably with other SR proteins—for pre-mRNA splicing in vitro, and it regulates various alternative splicing events. Dysregulation of SRSF1 expression contribute…