Tsuyoshi E. Maruyama
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View article: Production and Storage of Male-Sterile Somatic Embryos of Sugi (Japanese Cedar, Cryptomeria japonica) at Temperatures Above Freezing
Production and Storage of Male-Sterile Somatic Embryos of Sugi (Japanese Cedar, Cryptomeria japonica) at Temperatures Above Freezing Open
Sugi-pollinosis poses a significant socioeconomic and public health concern in Japanese society. Consequently, the use of male-sterile plants (pollen-free plants or PFPs) is anticipated in reforestation efforts. In this context, we develop…
View article: Propagation of Hinoki Cypress (Chamaecyparis obtusa) Through Tissue Culture Technique as a Sustainable Method for Mass Cloning of Selected Trees
Propagation of Hinoki Cypress (Chamaecyparis obtusa) Through Tissue Culture Technique as a Sustainable Method for Mass Cloning of Selected Trees Open
Propagation of hinoki cypress (Japanese cypress, Chamaecyparis obtusa, Cupressaceae) through adventitious bud multiplication was performed using leaf-segment explants from cutting plants of selected adult trees. Explants were successfully …
View article: Effect of Culture Temperatures on the Initial Growth Performance of Seedlings Germinated from Cryostored Seeds of a Tropical Tree Parkia nitida Miq. (Fabaceae, Mimosoideae)
Effect of Culture Temperatures on the Initial Growth Performance of Seedlings Germinated from Cryostored Seeds of a Tropical Tree Parkia nitida Miq. (Fabaceae, Mimosoideae) Open
Seedling growth is one of the most important stages for the establishment of natural and artificial regeneration. For the first time, the initial growth and biomass allocation of seedlings germinated from cryostored seeds of Parkia nitida …
View article: CRISPR/Cas9-mediated disruption of<i>CjACOS5</i>confers no-pollen formation on sugi trees (<i>Cryptomeria japonica</i>D. Don)
CRISPR/Cas9-mediated disruption of<i>CjACOS5</i>confers no-pollen formation on sugi trees (<i>Cryptomeria japonica</i>D. Don) Open
Sugi ( Cryptomeria japonica D. Don) is an economically important coniferous tree in Japan. However, abundant sugi pollen grains are dispersed and transported by the wind each spring and cause a severe pollen allergy syndrome (Japanese ceda…
View article: An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis
An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis Open
Sugi (Japanese cedar, Cryptomeria japonica ) is the most important forestry tree species in Japan, covering 44% of the total artificial forest area. Large amounts of pollen released from these forests each spring cause allergic reactions i…
View article: Plant Regeneration and In Vitro Growth Performance of Male-Sterile Somatic Plantlets of Sugi (Japanese Cedar, Cryptomeria japonica) Derived from Different Embryogenic Cell Lines
Plant Regeneration and In Vitro Growth Performance of Male-Sterile Somatic Plantlets of Sugi (Japanese Cedar, Cryptomeria japonica) Derived from Different Embryogenic Cell Lines Open
With the spread of pollinosis caused by sugi (Japanese cedar, Cryptomeria japonica) pollen, the use of pollen-free somatic seedlings of sugi is expected in Japan. However, there is a lack of knowledge on the relationship between the abilit…
View article: Marker-Assisted Selection for Pollen-Free Somatic Plants of Sugi (Japanese Cedar, Cryptomeria japonica): A Simple and Effective Methodology for Selecting Male-Sterile Mutants With ms1-1 and ms1-2
Marker-Assisted Selection for Pollen-Free Somatic Plants of Sugi (Japanese Cedar, Cryptomeria japonica): A Simple and Effective Methodology for Selecting Male-Sterile Mutants With ms1-1 and ms1-2 Open
Pollen allergy caused by sugi (Japanese cedar, Cryptomeria japonica ) is a serious problem in Japan. One of the measures against pollinosis is the use of male-sterile plants (MSPs; pollen-free plants). In this context, the development of a…
View article: Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, Cryptomeria japonica (Thunb. ex L.f.) D. Don)
Factors Influencing Somatic Embryo Maturation in Sugi (Japanese Cedar, Cryptomeria japonica (Thunb. ex L.f.) D. Don) Open
This paper presents the results of several experiments identifying basal salts (BS) contained in maturation medium, polyethylene glycol (PEG) concentration, abscisic acid (ABA) concentration, additional supplementation with potassium chlor…
View article: Somatic Embryogenesis Initiation in Sugi (Japanese Cedar, Cryptomeria japonica D. Don): Responses from Male-Fertile, Male-Sterile, and Polycross-Pollinated-Derived Seed Explants
Somatic Embryogenesis Initiation in Sugi (Japanese Cedar, Cryptomeria japonica D. Don): Responses from Male-Fertile, Male-Sterile, and Polycross-Pollinated-Derived Seed Explants Open
This study aimed to obtain information from several embryogenic cell (EC) genotypes analyzing the factors that affect somatic embryogenesis (SE) initiation in sugi (Cryptomeria japonica, Cupressaceae) to apply them in the improvement of pr…
View article: Genotype and transcriptome effects on somatic embryogenesis in Cryptomeria japonica
Genotype and transcriptome effects on somatic embryogenesis in Cryptomeria japonica Open
Somatic embryogenesis (SE), which is in vitro regeneration of plant bodies from somatic cells, represents a useful means of clonal propagation and genetic engineering of forest trees. While protocols to obtain calluses and induce regenerat…
View article: Somatic Embryogenesis and Plant Regeneration from Sugi (Japanese Cedar, Cryptomeria japonica D. Don, Cupressaceae) Seed Families by Marker Assisted Selection for the Male Sterility Allele ms1
Somatic Embryogenesis and Plant Regeneration from Sugi (Japanese Cedar, Cryptomeria japonica D. Don, Cupressaceae) Seed Families by Marker Assisted Selection for the Male Sterility Allele ms1 Open
One of the possible countermeasures for pollinosis caused by sugi (Cryptomeria japonica), a serious public health problem in Japan, is the use of male sterile plants (MSPs; pollen-free plants). However, the production efficiencies of MSPs …
View article: Progress in Somatic Embryogenesis of Japanese Pines
Progress in Somatic Embryogenesis of Japanese Pines Open
Somatic embryogenesis (SE) in not only one of the most promising techniques for mass propagation of selected trees, but also is a valuable tool for basic research studies in cell biology and genetic engineering, and it allows the long-term…