Tsvetelina Paunova‐Krasteva
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View article: Biofilm Formation of Pseudomonas aeruginosa in Cystic Fibrosis: Mechanisms of Persistence, Adaptation, and Pathogenesis
Biofilm Formation of Pseudomonas aeruginosa in Cystic Fibrosis: Mechanisms of Persistence, Adaptation, and Pathogenesis Open
Cystic fibrosis (CF) is a life-limiting autosomal recessive disorder affecting a large number of individuals in Europe. The disease arises from mutations in the CFTR gene encoding the cystic fibrosis transmembrane conductance regulator, a …
View article: Cationic Polymer Micelles as Carriers of Bioactive Sesquiterpene Lactones from Inula Helenium L. for Effective Treatment of Bacterial Biofilms
Cationic Polymer Micelles as Carriers of Bioactive Sesquiterpene Lactones from Inula Helenium L. for Effective Treatment of Bacterial Biofilms Open
Objectives: Nanosized polymeric micelles (PMs) with an average size of about 80 nm and moderately positive ζ potential, based on an amphiphilic poly(4-methyl-piperazin-1-yl)-propenone)-b-polylactide (PMPP-PLA) block copolymer, were prepare…
View article: Characterization of a Vaginal Limosilactobacillus Strain Producing Anti-Virulence Postbiotics: A Potential Probiotic Candidate
Characterization of a Vaginal Limosilactobacillus Strain Producing Anti-Virulence Postbiotics: A Potential Probiotic Candidate Open
The search for probiotics to help limit antibiotic resistance is a major scientific challenge. The exploration of Lactobacillus postbiotics represents a promising approach to prevent pathogen invasion. With this aim, Limosilactobacillus fe…
View article: The Effect of Quercetin Loading in Polylactic Acid-Based Electrospun Fibers on Their Antioxidant, Antibacterial and Antitumor Properties
The Effect of Quercetin Loading in Polylactic Acid-Based Electrospun Fibers on Their Antioxidant, Antibacterial and Antitumor Properties Open
Quercetin (QUE) is a bioactive flavonoid that is naturally present in various fruits and possesses many pharmacological activities. Despite its health benefits, the bioavailability of quercetin is relatively low due to its crystalline form…
View article: Cosmetic Potential of Haberlea rhodopensis Extracts and Extracellular Vesicles in Human Fibroblast Cells
Cosmetic Potential of Haberlea rhodopensis Extracts and Extracellular Vesicles in Human Fibroblast Cells Open
Skin ageing is a complex biological process influenced by cellular senescence, oxidative stress, and extracellular matrix degradation. Emerging evidence suggests that plant-derived bioactive compounds and extracellular vesicles (EVs) play …
View article: New Halophilic Community Degrades Plastics: A Metagenomic Study
New Halophilic Community Degrades Plastics: A Metagenomic Study Open
Biodegradation is an advanced method for reducing plastic waste in the environment, involving the participation of microbial communities with plastic-degrading properties. Our study presents a novel halophilic community isolated from the p…
View article: Phytochemical Characterization and Anti-Biofilm Activity of Primula veris L. Roots
Phytochemical Characterization and Anti-Biofilm Activity of Primula veris L. Roots Open
In this study, three new undescribed triterpene saponins named primulasaponin III–V (19–21) were isolated from the roots of Primula veris L. of Bulgarian origin together with the known primulasaponin I. Their structures were elucidated via…
View article: Diversification of Pseudomonas aeruginosa After Inhaled Tobramycin Therapy of Cystic Fibrosis Patients: Genotypic and Phenotypic Characteristics of Paired Pre- and Post-Treatment Isolates
Diversification of Pseudomonas aeruginosa After Inhaled Tobramycin Therapy of Cystic Fibrosis Patients: Genotypic and Phenotypic Characteristics of Paired Pre- and Post-Treatment Isolates Open
This study examines the impact of inhaled tobramycin therapy on the within-host changes in P. aeruginosa strains isolated from Bulgarian patients with CF prior to and post treatment. Genotypic comparison by RAPD-PCR indicated that most of …
View article: What We Still Don’t Know About Biofilms—Current Overview and Key Research Information
What We Still Don’t Know About Biofilms—Current Overview and Key Research Information Open
The existence of biofilms as heterogeneous structures, comprising bacterial cells embedded in a self-synthesized polymeric matrix and adhered to biotic and abiotic surfaces, exhibiting high antibiotic tolerance, is a fact that, in recent y…
View article: Gram Negative Biofilms: Structural and Functional Responses to Destruction by Antibiotic-Loaded Mixed Polymeric Micelles
Gram Negative Biofilms: Structural and Functional Responses to Destruction by Antibiotic-Loaded Mixed Polymeric Micelles Open
Biofilms are a well-known multifactorial virulence factor with a pivotal role in chronic bacterial infections. Their pathogenicity is determined by the combination of strain-specific mechanisms of virulence and the biofilm extracellular ma…
View article: Green Synthesized Composite AB-Polybenzimidazole/TiO2 Membranes with Photocatalytic and Antibacterial Activity
Green Synthesized Composite AB-Polybenzimidazole/TiO2 Membranes with Photocatalytic and Antibacterial Activity Open
Novel AB-Polybenzimidazole (AB-PBI)/TiO2 nanocomposite membranes have been prepared using a synthetic green chemistry approach. Modified Eaton’s reagent (methansulfonic acid/P2O5) was used as both reaction media for microwave-assisted synt…
View article: Concept of a Cyber–Physical System for Control of a Self-Cleaning Aquaponic Unit
Concept of a Cyber–Physical System for Control of a Self-Cleaning Aquaponic Unit Open
The article aims to present a cyber–physical system (CPS) to support the cultivation of aquaculture in a closed aquaponic system using the deep-water culture (DWC) method. The CPS uses precision sensors as TriOxmatic 700 IQ (for dissolved …
View article: Integrated Biofilm Dispersion and Virulence Responsiveness for Targeted Treatment of <i>Pseudomonas aeruginosa</i> Infection in Lungs
Integrated Biofilm Dispersion and Virulence Responsiveness for Targeted Treatment of <i>Pseudomonas aeruginosa</i> Infection in Lungs Open
The self‐organization of microbes into biofilms provides multiple benefits including tolerance to mechanical stress and resistance to immune defences and antibiotics. Coupled to a compromised mucociliary function, these traits have dire co…
View article: The Isolation, Identification and Characterization of a Wild-Type Strain Pseudomonas aeruginosa PM1012 from the Cloacal Microbiota of a Common Wall Lizard (Podarcis muralis Laurenti, 1768)
The Isolation, Identification and Characterization of a Wild-Type Strain Pseudomonas aeruginosa PM1012 from the Cloacal Microbiota of a Common Wall Lizard (Podarcis muralis Laurenti, 1768) Open
Pseudomonas aeruginosa is a ubiquitous environmental Gram-negative bacterium and also an opportunistic pathogen for both humans and animals, causing acute or chronic infections. It has been frequently detected in healthy and diseased repti…
View article: Inula salicina L.: Insights into Its Polyphenolic Constituents and Biological Activity
Inula salicina L.: Insights into Its Polyphenolic Constituents and Biological Activity Open
In this study, UHPLC-HRMS analysis of the defatted methanol extract obtained from Inula salicina L. led to the identification of 58 compounds—hydroxycinnamic and hydroxybenzoic acids and their glycosides, acylquinic and caffeoylhexaric aci…
View article: Anti-Biofilm and Anti-Quorum-Sensing Activity of Inula Extracts: A Strategy for Modulating Chromobacterium violaceum Virulence Factors
Anti-Biofilm and Anti-Quorum-Sensing Activity of Inula Extracts: A Strategy for Modulating Chromobacterium violaceum Virulence Factors Open
The formation of microbial biofilm is a self-organizing process among bacterial cells, regulated by quorum-sensing (QS) mechanisms, contributing to development of infections. These processes, either separately or in combination, significan…
View article: Pathogenic Potential of Opportunistic Gram-Negative Bacteria Isolated from the Cloacal Microbiota of Free-Living Reptile Hosts Originating from Bulgaria
Pathogenic Potential of Opportunistic Gram-Negative Bacteria Isolated from the Cloacal Microbiota of Free-Living Reptile Hosts Originating from Bulgaria Open
Reptiles are known to be asymptomatic carriers of various zoonotic pathogens. A number of Gram-negative opportunistic commensals are causative agents of bacterial infections in immunocompromised or stressed hosts and are disseminated by re…
View article: An Overview of Biofilm-Associated Infections and the Role of Phytochemicals and Nanomaterials in Their Control and Prevention
An Overview of Biofilm-Associated Infections and the Role of Phytochemicals and Nanomaterials in Their Control and Prevention Open
Biofilm formation is considered one of the primary virulence mechanisms in Gram-positive and Gram-negative pathogenic species, particularly those responsible for chronic infections and promoting bacterial survival within the host. In recen…
View article: From Traditional Dairy Product “Katak” to Beneficial Lactiplantibacillus plantarum Strains
From Traditional Dairy Product “Katak” to Beneficial Lactiplantibacillus plantarum Strains Open
Traditional milk products, widely consumed in many countries for centuries, have been drawing renewed attention in recent years as sources of bacteria with possible bioprotective properties. One such product for which only limited informat…
View article: Effects of Aromatic Compounds Degradation on Bacterial Cell Morphology
Effects of Aromatic Compounds Degradation on Bacterial Cell Morphology Open
The aim of the present study was to evaluate in parallel the capacity of three bacterial strains originating from oil-polluted soils to degrade monoaromatic compounds and the alterations in the bacterial cell morphology as a result of the …
View article: Ultra-Short Laser-Assisted Micro-Structure Formations on Mg/Zn Double-Doped Calcium Phosphate Ceramics for Enhanced Antimicrobial Activity
Ultra-Short Laser-Assisted Micro-Structure Formations on Mg/Zn Double-Doped Calcium Phosphate Ceramics for Enhanced Antimicrobial Activity Open
Bacterial infection is one of the most common and harmful medical issues following the implantation of materials and devices in the body leading to antibiotic resistance of diverse bacterial strains. In this work, a novel approach is prese…
View article: Multiple cry Genes in Bacillus thuringiensis Strain BTG Suggest a Broad-Spectrum Insecticidal Activity
Multiple cry Genes in Bacillus thuringiensis Strain BTG Suggest a Broad-Spectrum Insecticidal Activity Open
The properties of Bacillus thuringiensis strains as a biopesticide with potent action against moths, beetles, and mosquitoes have been known for decades, with individual subspecies showing specific activity against a particular pest. The a…
View article: Chromobacterium Violaceum: A Model for Evaluating the Anti-Quorum Sensing Activities of Plant Substances
Chromobacterium Violaceum: A Model for Evaluating the Anti-Quorum Sensing Activities of Plant Substances Open
In the new antibiotic era, the exponential increase in multiresistant bacterial strains has become the main global health problem. Many researchers have focused their efforts on exploring novel or combined strategies for combating bacteria…
View article: Chromobacterium violaceum: Model for Evaluating Anti-Quorum Sensing Activity of Plant Substances
Chromobacterium violaceum: Model for Evaluating Anti-Quorum Sensing Activity of Plant Substances Open
In the new antibiotic era, the exponential increase of multiresistant bacterial strains become the main global health problem. Many researchers focused their efforts to explore novel or combined strategies for combating bacterial resistanc…
View article: Polyphasic characterisation of Microcoleus autumnalis (Gomont, 1892) Strunecky, Komarek &amp; J.R.Johansen, 2013 (Oscillatoriales, Cyanobacteria) using a metabolomic approach as a complementary tool
Polyphasic characterisation of Microcoleus autumnalis (Gomont, 1892) Strunecky, Komarek & J.R.Johansen, 2013 (Oscillatoriales, Cyanobacteria) using a metabolomic approach as a complementary tool Open
As a result of the continuous revision of cyanobacterial taxonomy, Phormidium autumnale (Agardh) Trevisan ex Gomont, 1892 has been transferred to the genus Microcoleus as Microcoleus autumnalis (Gomont, 1892) Strunecky, Komárek & J.R.Johan…
View article: Ciprofloxacin-Loaded Mixed Polymeric Micelles as Antibiofilm Agents
Ciprofloxacin-Loaded Mixed Polymeric Micelles as Antibiofilm Agents Open
In this work, mixed polymeric micelles (MPMs) based on a cationic poly(2-(dimethylamino)ethyl methacrylate)-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA29-b-PCL70-b-PDMAEMA29) and a non-ionic poly(ethylene ox…
View article: A Thermostable Lipase Isolated from Brevibacillus thermoruber Strain 7 Degrades Ɛ-Polycaprolactone
A Thermostable Lipase Isolated from Brevibacillus thermoruber Strain 7 Degrades Ɛ-Polycaprolactone Open
The tremendous problem with plastic waste accumulation has determined an interest in biodegradation by effective degraders and their enzymes, such as thermophilic enzymes, which are characterized by high catalytic rates, thermostability, a…
View article: Bioactive Azadirachta indica and Melia azedarach leaves extracts with anti-SARS-CoV-2 and antibacterial activities
Bioactive Azadirachta indica and Melia azedarach leaves extracts with anti-SARS-CoV-2 and antibacterial activities Open
The leaves of Azadirachta indica L. and Melia azedarach L., belonging to Meliaceae family, have been shown to have medicinal benefits and are extensively employed in traditional folk medicine. Herein, HPLC analysis of the ethyl acetate fra…
View article: Effects of cationic polymers on the viability of microbial biofilms
Effects of cationic polymers on the viability of microbial biofilms Open
Introduction: The number of published biofilm studies and novel ways for studying them has risen dramatically in recent years, ow-ing to the broad application of biofilms in medicine. Some bacteria develop biofilms that are highly resistan…
View article: Ɛ-Polycaprolactone degradation by a thermostable lipase isolated from Brevibacillus thermoruber strain 7
Ɛ-Polycaprolactone degradation by a thermostable lipase isolated from Brevibacillus thermoruber strain 7 Open
An exponentially increasing accumulation of plastic wastes in the environment represents one of the biggest problems of our society that could be resolved by a biodegradation by enough effective degraders and their enzymes. The high cataly…