Tyler S. Klann
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View article: Machine learning methods for predicting guide RNA effects in CRISPR epigenome editing experiments
Machine learning methods for predicting guide RNA effects in CRISPR epigenome editing experiments Open
CRISPR epigenomic editing technologies enable functional interrogation of non-coding elements. However, current computational methods for guide RNA (gRNA) design do not effectively predict the power potential, molecular and cellular impact…
View article: Genome-wide annotation of gene regulatory elements linked to cell fitness
Genome-wide annotation of gene regulatory elements linked to cell fitness Open
Noncoding regulatory elements control gene expression and thus govern nearly all biological processes. Epigenomic profiling assays have identified millions of putative regulatory elements, but systematically determining the function of tho…
View article: Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens
Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens Open
Technologies to reprogram cell-type specification have revolutionized the fields of regenerative medicine and disease modeling. Currently, the selection of fate-determining factors for cell reprogramming applications is typically a laborio…
View article: Genome-wide CRISPR Screen to Identify Genes that Suppress Transformation in the Presence of Endogenous KrasG12D
Genome-wide CRISPR Screen to Identify Genes that Suppress Transformation in the Presence of Endogenous KrasG12D Open
Cooperating gene mutations are typically required to transform normal cells enabling growth in soft agar or in immunodeficient mice. For example, mutations in Kras and transformation-related protein 53 ( Trp53 ) are known to transform a va…
View article: CRISPR-based methods for high-throughput annotation of regulatory DNA
CRISPR-based methods for high-throughput annotation of regulatory DNA Open
View article: CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome
CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome Open
View article: 525. Directed Molecular Evolution of Transcription Factors in Mammalian Cells for Enhanced Directed Cell Differentiation
525. Directed Molecular Evolution of Transcription Factors in Mammalian Cells for Enhanced Directed Cell Differentiation Open
The advent of cellular reprogramming has introduced new possibilities for regenerative medicine, drug discovery, and controlling cellular behavior. Recent studies have identified many natural transcription factors that are capable of direc…