Upasana Roy
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View article: Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination
Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination Open
Rad51 catalyzes the DNA pairing reactions that take place during homologous recombination (HR), and HR must be tightly regulated to ensure physiologically appropriate outcomes. Rad54 is an ATP-dependent DNA motor protein that stimulates Ra…
View article: Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination
Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination Open
SUMMARY Rad51 catalyzes the DNA pairing reactions that take place during homologous recombination (HR), and HR must be tightly regulated to ensure physiologically appropriate outcomes. Rad54 is an ATP-dependent DNA motor protein that stimu…
View article: Computed structures of core eukaryotic protein complexes
Computed structures of core eukaryotic protein complexes Open
Deep learning for protein interactions The use of deep learning has revolutionized the field of protein modeling. Humphreys et al . combined this approach with proteome-wide, coevolution-guided protein interaction identification to conduct…
View article: Structures of core eukaryotic protein complexes
Structures of core eukaryotic protein complexes Open
Protein-protein interactions play critical roles in biology, but despite decades of effort, the structures of many eukaryotic protein complexes are unknown, and there are likely many interactions that have not yet been identified. Here, we…
View article: The Role of the Rad55–Rad57 Complex in DNA Repair
The Role of the Rad55–Rad57 Complex in DNA Repair Open
Homologous recombination (HR) is a mechanism conserved from bacteria to humans essential for the accurate repair of DNA double-stranded breaks, and maintenance of genome integrity. In eukaryotes, the key DNA transactions in HR are catalyze…
View article: Rad51 paralog complex Rad55–Rad57 acts as a molecular chaperone during homologous recombination. Roy et al.
Rad51 paralog complex Rad55–Rad57 acts as a molecular chaperone during homologous recombination. Roy et al. Open
We investigated the role of Rad51 paralog complex Rad55–Rad57 in homologous recombination. We visualized Rad55–Rad57 dynamics at the single–molecule level, in real–time using total internal reflection fluorescence microscopy (TIRFM). Our d…
View article: Rad51 paralog complex Rad55–Rad57 acts as a molecular chaperone during homologous recombination
Rad51 paralog complex Rad55–Rad57 acts as a molecular chaperone during homologous recombination Open
Summary Homologous recombination (HR) is essential for the maintenance of genome integrity. Rad51 paralogs fulfill a conserved, but undefined role in HR, and their mutations are associated with increased cancer risk in humans. Here, we use…
View article: Bypass of DNA interstrand crosslinks by a Rev1–DNA polymerase ζ complex
Bypass of DNA interstrand crosslinks by a Rev1–DNA polymerase ζ complex Open
DNA polymerase ζ (Pol ζ) and Rev1 are essential for the repair of DNA interstrand crosslink (ICL) damage. We have used yeast DNA polymerases η, ζ and Rev1 to study translesion synthesis (TLS) past a nitrogen mustard-based interstrand cross…
View article: Bypass of DNA Interstrand crosslinks by a Rev1-DNA polymerase ζ complex
Bypass of DNA Interstrand crosslinks by a Rev1-DNA polymerase ζ complex Open
DNA polymerase ζ (Pol ζ) and Rev1 are essential for the repair of DNA interstrand crosslink (ICL) damage. We have used yeast DNA polymerases η, ζ, and Rev1 to study translesion synthesis (TLS) past a nitrogen mustard-based ICL with an 8-at…
View article: Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair
Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair Open
Double strand breaks (DSBs) and interstrand crosslinks (ICLs) are toxic DNA lesions that can be repaired through multiple pathways, some of which involve shared proteins. One of these proteins, DNA Polymerase θ (Pol θ), coordinates a mutag…
View article: Structural mechanism of DNA interstrand cross-link unhooking by the bacterial FAN1 nuclease
Structural mechanism of DNA interstrand cross-link unhooking by the bacterial FAN1 nuclease Open
DNA interstrand cross-links (ICLs) block the progress of the replication and transcription machineries and can weaken chromosomal stability, resulting in various diseases. FANCD2-FANCI-associated nuclease (FAN1) is a conserved structure-sp…
View article: Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair
Drosophila DNA polymerase theta utilizes both helicase-like and polymerase domains during microhomology-mediated end joining and interstrand crosslink repair Open
Double strand breaks (DSBs) and interstrand crosslinks (ICLs) are toxic DNA lesions that can be repaired through multiple pathways, some of which involve shared proteins. One of these proteins, DNA Polymerase θ (Pol θ), coordinates a mutag…
View article: The structure and duplex context of DNA interstrand crosslinks affects the activity of DNA polymerase η
The structure and duplex context of DNA interstrand crosslinks affects the activity of DNA polymerase η Open
Several important anti-tumor agents form DNA interstrand crosslinks (ICLs), but their clinical efficiency is counteracted by multiple complex DNA repair pathways. All of these pathways require unhooking of the ICL from one strand of a DNA …