Ursula Stochaj
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View article: Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to Detect Newly Synthesized Proteins in Cells and their Secretome v1
Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to Detect Newly Synthesized Proteins in Cells and their Secretome v1 Open
Cells respond to physiological or pathological stimuli by altering the composition of the proteins they produce. This adaptation includes changes to newly translated polypeptides that are destined for intracellular compartments or secretio…
View article: Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome.
Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome. Open
Our work demonstrates the power of a modified BONCAT protocol to study newly produced proteins in growing cells and their secretome. This method will be useful to examine the proteome and secretome changes that are linked to the altered pe…
View article: Pifithrin-µ Induces Stress Granule Formation, Regulates Cell Survival, and Rewires Cellular Signaling
Pifithrin-µ Induces Stress Granule Formation, Regulates Cell Survival, and Rewires Cellular Signaling Open
(1) Background: Stress granules (SGs) are cytoplasmic protein-RNA condensates that assemble in response to various insults. SG production is driven by signaling pathways that are relevant to human disease. Compounds that modulate SG charac…
View article: Silica-coated LiYF<sub>4</sub>:Yb<sup>3+</sup>, Tm<sup>3+</sup> upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells
Silica-coated LiYF<sub>4</sub>:Yb<sup>3+</sup>, Tm<sup>3+</sup> upconverting nanoparticles are non-toxic and activate minor stress responses in mammalian cells Open
Silica-coated lanthanide-doped upconverting nanoparticles (UCNPs) are internalized by mammalian cells. The bipyramidal UCNPs are non-toxic and elicit minor stress responses in a cell type-dependent fashion.
View article: Yeast profilin mutants inhibit classical nuclear import and alter the balance between actin and tubulin levels
Yeast profilin mutants inhibit classical nuclear import and alter the balance between actin and tubulin levels Open
Profilin is a small protein that controls actin polymerization in yeast and higher eukaryotes. In addition, profilin has emerged as a multifunctional protein that contributes to other processes in multicellular organisms. This study focuse…
View article: Oxidative stress and signaling through EGFR and PKA pathways converge on the nuclear transport factor RanBP1
Oxidative stress and signaling through EGFR and PKA pathways converge on the nuclear transport factor RanBP1 Open
Nuclear protein trafficking requires the soluble transport factor RanBP1. The subcellular distribution of RanBP1 is dynamic, as the protein shuttles between the nucleus and cytoplasm. To date, the signaling pathways regulating RanBP1 subce…
View article: Curcumin and butyrate induce fibroblast senescence without the emergence of fibrosis biomarkers
Curcumin and butyrate induce fibroblast senescence without the emergence of fibrosis biomarkers Open
Background: Small molecules have emerged as valuable tools to modulate cellular homeostasis and the changes associated with aging. In particular, the phytochemical curcumin elicits cytoprotective effects that promote human health and longe…
View article: Additional file 2 of Valosin containing protein (VCP): initiator, modifier, and potential drug target for neurodegenerative diseases
Additional file 2 of Valosin containing protein (VCP): initiator, modifier, and potential drug target for neurodegenerative diseases Open
Additional file 2: Supplemental Table 5. Clinical trials relevant to VCP disease.
View article: Multiple pathways promote microtubule stabilization in senescent intestinal epithelial cells
Multiple pathways promote microtubule stabilization in senescent intestinal epithelial cells Open
Intestinal epithelial cells are critical for gastrointestinal homeostasis. However, their function declines during aging. The aging-related loss of organ performance is largely driven by the increase in senescent cells. To date, the hallma…
View article: CD26 is a senescence marker associated with reduced immunopotency of human adipose tissue-derived multipotent mesenchymal stromal cells
CD26 is a senescence marker associated with reduced immunopotency of human adipose tissue-derived multipotent mesenchymal stromal cells Open
Introduction Human mesenchymal stromal cells (MSCs) have immunomodulatory, anti-inflammatory, and tolerogenic effects. Long-term in vitro expansion of MSCs to generate clinical grade products results in the accumulation of senescent-functi…
View article: Additional file 2 of CD26 is a senescence marker associated with reduced immunopotency of human adipose tissue-derived multipotent mesenchymal stromal cells
Additional file 2 of CD26 is a senescence marker associated with reduced immunopotency of human adipose tissue-derived multipotent mesenchymal stromal cells Open
Additional file 2. Excel file with RNA-seq data.
View article: Evaluation of Lanthanide-Doped Upconverting Nanoparticles for <i>in Vitro</i> and <i>in Vivo</i> Applications
Evaluation of Lanthanide-Doped Upconverting Nanoparticles for <i>in Vitro</i> and <i>in Vivo</i> Applications Open
Because of their unique physicochemical properties, lanthanide-doped upconverting nanoparticles (Ln-UCNPs) have exceptional potential for biological applications. However, the use in biological systems is hampered by the limited understand…
View article: The Co-Chaperone HspBP1 Is a Novel Component of Stress Granules that Regulates Their Formation
The Co-Chaperone HspBP1 Is a Novel Component of Stress Granules that Regulates Their Formation Open
The co-chaperone HspBP1 interacts with members of the hsp70 family, but also provides chaperone-independent functions. We report here novel biological properties of HspBP1 that are relevant to the formation of cytoplasmic stress granules (…
View article: Nucleolar Organization and Functions in Health and Disease
Nucleolar Organization and Functions in Health and Disease Open
The nucleolus is a prominent, membraneless compartment found within the nucleus of eukaryotic cells. It forms around ribosomal RNA (rRNA) genes, where it coordinates the transcription, processing, and packaging of rRNA to produce ribosomal…
View article: Gold nanoclusters elicit homeostatic perturbations in glioblastoma cells and adaptive changes of lysosomes
Gold nanoclusters elicit homeostatic perturbations in glioblastoma cells and adaptive changes of lysosomes Open
Unique physicochemical features place gold nanoclusters at the forefront of nanotechnology for biological and biomedical applications. To date, information on the interactions of gold nanoclusters with biological macromolecules is limited …
View article: Exploring near-infrared absorbing nanocarriers to overcome cancer drug resistance
Exploring near-infrared absorbing nanocarriers to overcome cancer drug resistance Open
One of the major obstacles of successful cancer therapy is cancer drug resistance. The unique tools and applications developed by nanomedicine provide new approaches to surmount this common limitation of current treatment regimens. Nanocar…
View article: Inhibition of glioblastoma cell proliferation, invasion, and mechanism of action of a novel hydroxamic acid hybrid molecule
Inhibition of glioblastoma cell proliferation, invasion, and mechanism of action of a novel hydroxamic acid hybrid molecule Open
Glioblastoma multiforme is one of the most aggressive brain tumors and current therapies with temozolomide or suberoylanilide hydroxamic acid (SAHA, vorinostat) show considerable limitations. SAHA is a histone deacetylase (HDAC) inhibitor …
View article: Defining the short-term effects of pharmacological 5′-AMP activated kinase modulators on mitochondrial polarization, morphology and heterogeneity
Defining the short-term effects of pharmacological 5′-AMP activated kinase modulators on mitochondrial polarization, morphology and heterogeneity Open
Background Under aerobic growth conditions, mitochondria are the major producers of cellular ATP and crucial for the proper performance of organs and tissues. This applies especially to cells with high energy demand, such as the renal prox…
View article: Cytoplasmic RNA Granules in Somatic Maintenance
Cytoplasmic RNA Granules in Somatic Maintenance Open
Cytoplasmic RNA granules represent subcellular compartments that are enriched in protein-bound RNA species. RNA granules are produced by evolutionary divergent eukaryotes, including yeast, mammals, and plants. The functions of cytoplasmic …
View article: Supplementary Material for: Cytoplasmic RNA Granules in Somatic Maintenance
Supplementary Material for: Cytoplasmic RNA Granules in Somatic Maintenance Open
Cytoplasmic RNA granules represent subcellular compartments that are enriched in protein-bound RNA species. RNA granules are produced by evolutionary divergent eukaryotes, including yeast, mammals, and plants. The functions of cytoplasmic …
View article: Mitochondrial Oxidative Stress Reduces the Immunopotency of Mesenchymal Stromal Cells in Adults With Coronary Artery Disease
Mitochondrial Oxidative Stress Reduces the Immunopotency of Mesenchymal Stromal Cells in Adults With Coronary Artery Disease Open
Rationale: Mesenchymal stromal cells (MSCs) are promising therapeutic strategies for coronary artery disease; however, donor-related variability in cell quality is a main cause of discrepancies in preclinical studies. In vitro, MSCs from i…
View article: Erratum to: Age, atherosclerosis and type 2 diabetes reduce human mesenchymal stromal cell-mediated T-cell suppression
Erratum to: Age, atherosclerosis and type 2 diabetes reduce human mesenchymal stromal cell-mediated T-cell suppression Open
Atherosclerosis is an age-associated, multifactorial process driven by immune activation and inflammation. Ongoing clinical trials aim to establish the role of mesenchymal stromal cells (MSCs) as therapeutic agents in atherosclerosis. The …
View article: Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells
Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells Open
Cancer cells can take up gold nanoparticles of different morphologies. These particles interact with the plasma membrane and often travel to intracellular organelles. Among organelles, the nucleus is especially susceptible to the damage th…
View article: Consistent sex-dependent effects of PKMζ gene ablation and pharmacological inhibition on the maintenance of referred pain
Consistent sex-dependent effects of PKMζ gene ablation and pharmacological inhibition on the maintenance of referred pain Open
Background Persistently active PKMζ has been implicated in maintaining spinal nociceptive sensitization that underlies pain hypersensitivity. However, evidence for PKMζ in the maintenance of pain hypersensitivity comes exclusively from sho…
View article: Quantitative analysis of the interplay between hsc70 and its co-chaperone HspBP1
Quantitative analysis of the interplay between hsc70 and its co-chaperone HspBP1 Open
Background. Chaperones and their co-factors are components of a cellular network; they collaborate to maintain proteostasis under normal and harmful conditions. In particular, hsp70 family members and their co-chaperones are essential to r…