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View article: Analysis of essential genes in <i>Clostridioides difficile</i> by CRISPRi and Tn-seq
Analysis of essential genes in <i>Clostridioides difficile</i> by CRISPRi and Tn-seq Open
Essential genes are interesting in their own right and as potential antibiotic targets. To date, only one report has identified essential genes on a genome-wide scale in Clostridioides difficile , a problematic pathogen for which treatment…
View article: Analysis of Essential Genes in <i>Clostridioides difficile</i> by CRISPRi and Tn-seq
Analysis of Essential Genes in <i>Clostridioides difficile</i> by CRISPRi and Tn-seq Open
Essential genes are interesting in their own right and as potential antibiotic targets. To date, only one report has identified essential genes on a genome-wide scale in Clostridioides difficile , a problematic pathogen for which treatment…
View article: Identification of a family of peptidoglycan transpeptidases reveals that <i>Clostridioides difficile</i> requires noncanonical cross-links for viability
Identification of a family of peptidoglycan transpeptidases reveals that <i>Clostridioides difficile</i> requires noncanonical cross-links for viability Open
Most bacteria are surrounded by a cell wall that contains peptidoglycan (PG), a large polymer composed of glycan strands held together by short peptide cross-links. There are two major types of cross-links, termed 4-3 and 3-3 based on the …
View article: Identification of a new family of peptidoglycan transpeptidases reveals atypical crosslinking is essential for viability in<i>Clostridioides difficile</i>
Identification of a new family of peptidoglycan transpeptidases reveals atypical crosslinking is essential for viability in<i>Clostridioides difficile</i> Open
Clostridioides difficile , the leading cause of antibiotic-associated diarrhea, relies primarily on 3-3 crosslinks created by L,D-transpeptidases (LDTs) to fortify its peptidoglycan (PG) cell wall. This is unusual, as in most bacteria the …
View article: Identification of <i>Clostridioides difficile</i> mutants with increased daptomycin resistance
Identification of <i>Clostridioides difficile</i> mutants with increased daptomycin resistance Open
Daptomycin is a cyclic lipopeptide antibiotic used to treat infections caused by some Gram-positive bacteria. Daptomycin disrupts synthesis of the peptidoglycan (PG) cell wall by inserting into the cytoplasmic membrane and binding multiple…
View article: The WalRK Two-Component System Is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile
The WalRK Two-Component System Is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile Open
The WalRK two-component system (TCS) is essential for coordinating synthesis and turnover of peptidoglycan in Firmicutes . We investigated the WalRK TCS in Clostridioides difficile , an important bacterial pathogen with an atypical cell en…
View article: The WalRK two-component system is essential for proper cell envelope biogenesis in <i>Clostridioides difficile</i>
The WalRK two-component system is essential for proper cell envelope biogenesis in <i>Clostridioides difficile</i> Open
The WalR-WalK two-component regulatory system (TCS) is found in all Firmicutes, where it regulates the expression of multiple genes required for remodeling the cell envelope during growth and division. Unlike most TCSs, WalRK is essential …
View article: DrpB (YedR) Is a Nonessential Cell Division Protein in Escherichia coli
DrpB (YedR) Is a Nonessential Cell Division Protein in Escherichia coli Open
A thorough understanding of bacterial cell division requires identifying and characterizing all of the proteins that participate in this process. Our discovery of DrpB brings us one step closer to this goal in E. coli .
View article: Lysozyme Resistance in Clostridioides difficile Is Dependent on Two Peptidoglycan Deacetylases
Lysozyme Resistance in Clostridioides difficile Is Dependent on Two Peptidoglycan Deacetylases Open
Clostridioides difficile is the leading cause of hospital-acquired diarrhea. C. difficile is highly resistant to lysozyme. We previously showed that the csfV operon is required for lysozyme resistance. Here, we used CRISPR-Cas9 mediated mu…
View article: Lysozyme resistance in<i>C. difficile</i>is dependent on two peptidoglycan deacetylases
Lysozyme resistance in<i>C. difficile</i>is dependent on two peptidoglycan deacetylases Open
Clostridioides (Clostridium) difficile is a major cause of hospital-acquired infections leading to antibiotic-associated diarrhea. C. difficile exhibits a very high level of resistance to lysozyme. Bacteria commonly resist lysozyme through…
View article: Activation of the Extracytoplasmic Function σ Factor σ <sup>P</sup> by β-Lactams in Bacillus thuringiensis Requires the Site-2 Protease RasP
Activation of the Extracytoplasmic Function σ Factor σ <sup>P</sup> by β-Lactams in Bacillus thuringiensis Requires the Site-2 Protease RasP Open
The discovery of antibiotics to treat bacterial infections has had a dramatic and positive impact on human health. However, shortly after the introduction of a new antibiotic, bacteria often develop resistance. The bacterial cell envelope …
View article: Activation of the Extracytoplasmic Function σ factor σ<sup>P</sup>by β-lactams in<i>Bacillus thuringiensis</i>requires the site-2 protease RasP
Activation of the Extracytoplasmic Function σ factor σ<sup>P</sup>by β-lactams in<i>Bacillus thuringiensis</i>requires the site-2 protease RasP Open
Bacteria can utilize alternative σ factors to regulate sets of genes in response to changes in the environment. The largest and most diverse group of alternative σ factors are the Extracytoplasmic Function (ECF) σ factors. σ P is an ECF σ …
View article: A Xylose-Inducible Expression System and a CRISPR Interference Plasmid for Targeted Knockdown of Gene Expression in Clostridioides difficile
A Xylose-Inducible Expression System and a CRISPR Interference Plasmid for Targeted Knockdown of Gene Expression in Clostridioides difficile Open
Clostridioides difficile has become the leading cause of hospital-acquired diarrhea in developed countries. A better understanding of the basic biology of this devastating pathogen might lead to novel approaches for preventing or treating …
View article: A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in<i>Clostridioides difficile</i>
A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in<i>Clostridioides difficile</i> Open
Here we introduce plasmids for xylose-regulated expression and repression of genes in Clostridioides difficile . The xylose-inducible expression vector allows for ~100-fold induction of an mCherryOpt reporter gene. Induction is titratable …
View article: Bacterial sensing: A putative amphipathic helix in RsiV is the switch for activating σV in response to lysozyme
Bacterial sensing: A putative amphipathic helix in RsiV is the switch for activating σV in response to lysozyme Open
Extra Cytoplasmic Function (ECF) σ factors are a diverse group of alternate σ factors bacteria use to respond to changes in the environment. The Bacillus subtilis ECF σ factor σV responds to lysozyme. In the absence of lysozyme, σV is held…
View article: Mtb PKNA/PKNB Dual Inhibition Provides Selectivity Advantages for Inhibitor Design To Minimize Host Kinase Interactions
Mtb PKNA/PKNB Dual Inhibition Provides Selectivity Advantages for Inhibitor Design To Minimize Host Kinase Interactions Open
Drug resistant tuberculosis (TB) infections are on the rise and antibiotics that inhibit Mycobacterium tuberculosis through a novel mechanism could be an important component of evolving TB therapy. Protein kinase A (PknA) and protein kinas…