Uzi Hadad
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Advancing Skin Rejuvenation Through Ultrasound-Enhanced Non-Invasive Delivery of Hyaluronic Acid Nanoparticles Open
High-molecular-weight hyaluronic acid (HMw-HA) is widely used for skin rejuvenation and anti-aging. However, its rapid degradation and <24 h cutaneous residence limit its therapeutic potential. Cross-linked HA fillers were developed to imp…
Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model Open
Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation…
Targeted siRNA Delivery Using Cetuximab‐Conjugated Starch for Epidermal Growth Factor Receptor‐Driven Head and Neck Squamous Cell Carcinoma Open
Small interfering RNA (siRNA) therapy holds significant potential to disrupt oncogenic signaling pathways by targeting specific messenger RNA (mRNA) sequences. However, its clinical application is limited by challenges in developing effect…
Enhancing the Efficacy of CAR-T Cell Production Using BX795 and Rosuvastatin in a Serum-Free Medium Open
Chimeric Antigen Receptor T-cell (CAR-T) therapy has emerged as a transformative approach for cancer treatment, demonstrating remarkable success in patients with relapsed and refractory hematological malignancies. However, challenges persi…
Relative Distribution of DnaA and DNA in Escherichia coli Cells as a Factor of Their Phenotypic Variability Open
Phenotypic variability in isogenic bacterial populations is a remarkable feature that helps them cope with external stresses, yet it is incompletely understood. This variability can stem from gene expression noise and/or the unequal partit…
Relative Distribution of DnaA and DNA in<i>Escherichia coli</i>Cells as a Factor of Their Phenotypic Variability Open
Phenotypic variability in isogenic bacterial populations helps them to cope with external stress. This variability can stem from gene expression noise and/or unequal partitioning of low-copy-number freely diffusing proteins during cell div…
The Potential of PIP3 in Enhancing Wound Healing Open
Given the role of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in modulating cellular processes such as proliferation, survival, and migration, we hypothesized its potential as a novel therapeutic agent for wound closure enhancement. In…
A Missense Variation in PHACTR2 Associates with Impaired Actin Dynamics, Dilated Cardiomyopathy, and Left Ventricular Non-Compaction in Humans Open
Dilated cardiomyopathy (DCM) with left ventricular non-compaction (LVNC) is a primary myocardial disease leading to contractile dysfunction, progressive heart failure, and excessive risk of sudden cardiac death. Using whole-exome sequencin…
Heat stress increases immune cell function in Hexacorallia Open
Climate change induced heat stress has increased coral bleaching events worldwide. Differentially regulated immune genes are one of the primary responses to heat stress suggesting that immune activation is critical. However, the cellular i…
Synergistic Activity of Anticancer Polyphenols Embedded in Amphiphilic Dendrimer Nanoparticles Open
Dendritic polymer nanoparticles (NPs) are promising vehicles for drug delivery. Most dendrimer polymer NPs, however, exhibit positive surface charge which make them, in many instances, cytotoxic. We constructed noncationic, amphiphilic den…
Context-dependent differences in the functional responses of Lactobacillaceae strains to fermentable sugars Open
Lactobacillaceae are Gram-positive rods, facultative anaerobes, and belong to the lactic acid bacteria (LAB) that frequently serve as probiotics. We systematically compared five LAB strains for the effects of different carbohydrates on the…
Candidate stem cell isolation and transplantation in Hexacorallia Open
Stem cells are the base for cell therapy due to their ability to self-renew, differentiate into other cell types, and live throughout the life of an organism. The initial cell therapy was bone marrow transplantation; bone marrow that conta…
Metaphase Cells Enrichment for Efficient Use in the Dicentric Chromosome Assay Open
The dicentric chromosome assay (DCA), is considered the ‘gold standard’ for radiation biodosimetry. Yet, DCA, as currently implemented, may be impractical for emergency response applications, especially when time is of the essence, owing t…
Defining the timeline of periostin upregulation in cardiac fibrosis following acute myocardial infarction in mice Open
After myocardial infarction (MI), the heart's reparative response to the ischemic insult and the related loss of cardiomyocytes involves cardiac fibrosis, in which the damaged tissue is replaced with a fibrous scar. Although the scar is es…
Yeast-based directed-evolution for high-throughput structural stabilization of G protein-coupled receptors (GPCRs) Open
The immense potential of G protein-coupled receptors (GPCRs) as targets for drug discovery is not fully realized due to the enormous difficulties associated with structure elucidation of these profoundly unstable membrane proteins. The exi…
A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state Open
The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology. However, the role that bacterial metabolites play in communicating with host cells during inflammatory diseases is poorly unde…
Functional Characterization of Hexacorallia Phagocytic Cells Open
Phagocytosis is the cellular defense mechanism used to eliminate antigens derived from dysregulated or damaged cells, and microbial pathogens. Phagocytosis is therefore a pillar of innate immunity, whereby foreign particles are engulfed an…
View article: Molecular-scale spatio-chemical control of the activating-inhibitory signal integration in NK cells
Molecular-scale spatio-chemical control of the activating-inhibitory signal integration in NK cells Open
Molecular devices for the spatial control of activating and inhibitory receptors help in their signal integration.
Distinct features of the Leishmania cap-binding protein LeishIF4E2 revealed by CRISPR-Cas9 mediated hemizygous deletion Open
Leishmania parasites cycle between sand-fly vectors and mammalian hosts adapting to alternating environments by stage-differentiation accompanied by changes in the proteome profiles. Translation regulation plays a central role in driving t…
Capillary bacterial migration on non-nutritive solid surfaces Open
Here we describe an additional type of bacterial migration in which bacterial cells migrate vertically across a non-nutritive solid surface carried by capillary forces. Unlike standard motility experiments, these were run on a glass slide …
Molecular and cellular correlates in Kv channel clustering: entropy-based regulation of cluster ion channel density Open
Scaffold protein-mediated ion channel clustering at unique membrane sites is important for electrical signaling. Yet, the mechanism(s) by which scaffold protein-ion channel interactions lead to channel clustering or how cluster ion channel…
View article: Molecular Scale Spatio-Chemical Control of the Activating-Inhibitory Signal Integration in NK Cells
Molecular Scale Spatio-Chemical Control of the Activating-Inhibitory Signal Integration in NK Cells Open
The role of the spatial juxtaposition between activating and inhibitory receptors in cytotoxic lymphocytes has been strongly debated in the context of the inhibition of immune signaling. The challenge in addressing this problem was so far …
View article: Mechanical Regulation of the Cytotoxic Activity of Natural Killer Cells
Mechanical Regulation of the Cytotoxic Activity of Natural Killer Cells Open
Mechanosensing has been recently explored for T cells and B cells and is believed to be part of their activation mechanism. Here, we explore the mechanosensing of the third type of lymphocytes – Natural Killer (NK) cells, by showing that t…
The Ebola-Glycoprotein Modulates the Function of Natural Killer Cells Open
The Ebola virus (EBOV) uses evasion mechanisms that directly interfere with host T-cell antiviral responses. By steric shielding of human leukocyte antigen class-1, the Ebola glycoprotein (GP) blocks interaction with T-cell receptors (TCRs…
Survival in acute myeloid leukemia is associated with NKp44 splice variants Open
NKp44 is a receptor encoded by the NCR2 gene, which is expressed by cytokine-activated natural killer (NK) cells that are involved in anti-AML immunity. NKp44 has three splice variants corresponding to NKp44ITIM+ (NKp44-1) and NKp44ITIM- (…