V. Šrajer
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View article: Time-Resolved Pump-Probe X-Ray Solution Scattering Capabilities at BioCARS 14 ID Beamline, Advanced Photon Source.
Time-Resolved Pump-Probe X-Ray Solution Scattering Capabilities at BioCARS 14 ID Beamline, Advanced Photon Source. Open
A fundamental problem in biological sciences is to connect biological function of a molecule with its structure. This is typically achieved by initiating a reaction in a biomolecule, and then following structural changes. Here we present t…
View article: Time-Resolved Macromolecular Crystallography Frontiers at BioCARS: Serial Laue Micro-Crystallography and Electric Field Jump
Time-Resolved Macromolecular Crystallography Frontiers at BioCARS: Serial Laue Micro-Crystallography and Electric Field Jump Open
BioCARS is a University of Chicago National User Facility with more than 25 years of scientific focus on synchrotron-based, dynamic studies in structural biology, initially using time-resolved protein crystallography and, more recently, ti…
View article: Laue-DIALS: open-source software for polychromatic X-ray diffraction data
Laue-DIALS: open-source software for polychromatic X-ray diffraction data Open
Most X-ray sources are inherently polychromatic. Polychromatic (“pink”) X-rays provide an efficient way to conduct diffraction experiments as many more photons can be used and large regions of reciprocal space can be probed without sample …
View article: Time-Resolved Macromolecular Crystallography Frontiers at BioCARS: Serial Laue Micro-Crystallography and Electric Field Jump
Time-Resolved Macromolecular Crystallography Frontiers at BioCARS: Serial Laue Micro-Crystallography and Electric Field Jump Open
BioCARS is a University of Chicago National User Facility with more than 20 years of scientific focus on synchrotron-based, dynamic studies in structural biology, initially using time-resolved protein crystallography and, more recently, ti…
View article: Time-Resolved Pump-Probe X-Ray Solution Scattering Capabilities at BioCARS 14 ID Beamline, Advanced Photon Source.
Time-Resolved Pump-Probe X-Ray Solution Scattering Capabilities at BioCARS 14 ID Beamline, Advanced Photon Source. Open
An essential problem in biological sciences is to connect biological function of a molecule with its structure. This is typically achieved by initiating a reaction in a biomolecule, and then following structural changes by methods such as …
View article: Determining the Mechanism of DJ-1 Using Mix-and-Inject Synchrotron Serial Crystallography
Determining the Mechanism of DJ-1 Using Mix-and-Inject Synchrotron Serial Crystallography Open
Determining the molecular basis of enzyme catalytic activity is a central challenge in biochemistry. Time-resolved serial X-ray crystallography using X-ray free electron (XFELs) and synchrotron sources allows enzyme reactions to be observe…
View article: Determining the Enzymatic Mechanism of DJ-1 Using a New Approach to Mix-and-Inject Synchrotron Serial Crystallography
Determining the Enzymatic Mechanism of DJ-1 Using a New Approach to Mix-and-Inject Synchrotron Serial Crystallography Open
Mix-and-inject serial crystallography (MISC) is a well established technique at X-ray Free Electron Lasers (XFELs) to capture snapshots of the progress of a reaction, typically between a protein and a ligand. Key to this method are special…
View article: Scaling and merging time-resolved pink-beam diffraction with variational inference
Scaling and merging time-resolved pink-beam diffraction with variational inference Open
Time-resolved x-ray crystallography (TR-X) at synchrotrons and free electron lasers is a promising technique for recording dynamics of molecules at atomic resolution. While experimental methods for TR-X have proliferated and matured, data …
View article: Laue-DIALS: Open-source software for polychromatic x-ray diffraction data
Laue-DIALS: Open-source software for polychromatic x-ray diffraction data Open
Most x-ray sources are inherently polychromatic. Polychromatic (“pink”) x-rays provide an efficient way to conduct diffraction experiments as many more photons can be used and large regions of reciprocal space can be probed without sample …
View article: Scaling and Merging Time-Resolved Laue Data with Variational Inference
Scaling and Merging Time-Resolved Laue Data with Variational Inference Open
Time-resolved X-ray crystallography (TR-X) at synchrotrons and free electron lasers is a promising technique for recording dynamics of molecules at atomic resolution. While experimental methods for TR-X have proliferated and matured, data …
View article: Laue-DIALS: open-source software for polychromatic X-ray diffraction data
Laue-DIALS: open-source software for polychromatic X-ray diffraction data Open
Most X-ray sources are inherently polychromatic. Polychromatic (“pink”) X-rays provide an efficient way to conduct diffraction experiments as many more photons can be used and large regions of reciprocal space can be probed without sample …
View article: Resolving DJ-1 Glyoxalase Catalysis Using Mix-and-Inject Serial Crystallography at a Synchrotron
Resolving DJ-1 Glyoxalase Catalysis Using Mix-and-Inject Serial Crystallography at a Synchrotron Open
DJ-1 (PARK7) is an intensively studied protein whose cytoprotective activities are dysregulated in multiple diseases. DJ-1 has been reported as having two distinct enzymatic activities in defense against reactive carbonyl species that are …
View article: BioCARS: Synchrotron facility for probing structural dynamics of biological macromolecules
BioCARS: Synchrotron facility for probing structural dynamics of biological macromolecules Open
A major goal in biomedical science is to move beyond static images of proteins and other biological macromolecules to the internal dynamics underlying their function. This level of study is necessary to understand how these molecules work …
View article: Materials related to: Direct visualization of electric field-stimulated ion conduction in a potassium channel
Materials related to: Direct visualization of electric field-stimulated ion conduction in a potassium channel Open
This deposition contains: PDB Validation Reports. For versions of record and additional data, including merged intensities or structure factor amplitudes, please consult PDB entries 8CTN, 8CU1, 8CU2, 8CU3, 8CU4 8CTS, 8CTT, 8CTU, 8CTV, 8CTW…
View article: Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering
Sample-minimizing co-flow cell for time-resolved pump–probe X-ray solution scattering Open
A fundamental problem in biological sciences is understanding how macromolecular machines work and how the structural changes of a molecule are connected to its function. Time-resolved techniques are vital in this regard and essential for …
View article: APS-U update and structural biology future directions
APS-U update and structural biology future directions Open
After 25 years in operation, the Advanced Photon Source (APS) is undergoing a massive upgrade.The current electron storage ring will be replaced with state-of-the art technology that will increase the brightness of the X-ray beams up to 50…
View article: Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature
Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature Open
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies. In the case of metalloenzymes…
View article: High-resolution Crystal Structures of Transient Intermediates in the Phytochrome Photocycle
High-resolution Crystal Structures of Transient Intermediates in the Phytochrome Photocycle Open
Phytochromes are red/far-red light photoreceptors in bacteria to plants, which elicit a variety of important physiological responses. They display a reversible photocycle between the resting (dark) Pr state and the light activated Pfr stat…
View article: High-resolution crystal structures of a myxobacterial phytochrome at cryo and room temperatures
High-resolution crystal structures of a myxobacterial phytochrome at cryo and room temperatures Open
Phytochromes (PHYs) are photoreceptor proteins first discovered in plants, where they control a variety of photomorphogenesis events. PHYs as photochromic proteins can reversibly switch between two distinct states: a red light (Pr) and a f…
View article: Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein
Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein Open
Visualizing a response to light Many biological processes depend on detecting and responding to light. The response is often mediated by a structural change in a protein that begins when absorption of a photon causes isomerization of a chr…
View article: Graphene-based microfluidics for serial crystallography
Graphene-based microfluidics for serial crystallography Open
A robust strategy for the incorporation of graphene films as a diffusion barrier in ultra-thin X-ray compatible microfluidic devices.
View article: Towards time-resolved serial crystallography in a microfluidic device
Towards time-resolved serial crystallography in a microfluidic device Open
Serial methods for crystallography have the potential to enable dynamic structural studies of protein targets that have been resistant to single-crystal strategies. The use of serial data-collection strategies can circumvent challenges ass…