Vage Markosyan
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View article: Biosafety Evaluation of a Chimeric Adenoviral Vector in Mini-Pigs: Insights into Immune Tolerance and Gene Therapy Potential
Biosafety Evaluation of a Chimeric Adenoviral Vector in Mini-Pigs: Insights into Immune Tolerance and Gene Therapy Potential Open
Background: The biosafety of gene therapy products remains a major challenge to their introduction into the clinic. In particular, the problem of immunogenicity of viral vectors is the focus of attention. Large animals such as pigs, whose …
View article: Morphological characteristics of the cerebral cortex of a mini-pig under conditions of gene therapy after experimental stroke
Morphological characteristics of the cerebral cortex of a mini-pig under conditions of gene therapy after experimental stroke Open
Aim. To study the effectiveness of preventive gene therapy (within 2 days) and gene therapy in the acute phase (after 4 hours) of ischemic stroke in mini-pigs using an autologous leucoconcentrate (AutoLeuc) enriched with recombinant genes …
Features of neuroglia at the epicenter of spinal cord contusion injury and at distant areas in mini-pigs Open
Aim . To determine the delayed (after 2 months) effect of spinal cord injury (SCI) in the lower thoracic region in the mini-pigs on the morphologic state of macro- and microglia in nearby and remote caudal areas. Materials and methods . Se…
View article: Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury Open
Background: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord rep…
View article: Gene technologies in ischemic stroke preclinical studies
Gene technologies in ischemic stroke preclinical studies Open
Ischemic stroke is one of the leading death causes and disability worldwide. The review is devoted to analysis of gene technologies application achievements in ischemic stroke experimental study. In experimental brain ischemia, genetic con…
View article: Model of small-focal ischemic cerebral infarction as a basis for the development of new methods of stroke therapy
Model of small-focal ischemic cerebral infarction as a basis for the development of new methods of stroke therapy Open
Background. Among the existing experimental models of cerebral stroke in large animals, there is no possibility to create a focal brain infarction of limited volume, which would provide an opportunity to evaluate both the endogenous potent…
View article: Autologous Genetically Enriched Leucoconcentrate in the Preventive and Acute Phases of Stroke Treatment in a Mini-Pig Model
Autologous Genetically Enriched Leucoconcentrate in the Preventive and Acute Phases of Stroke Treatment in a Mini-Pig Model Open
The natural limitations of regeneration in the CNS are major problems for the treatment of neurological disorders, including ischaemic brain strokes. Among the approaches being actively developed to inhibit post-ischaemic negative conseque…
New Therapy for Spinal Cord Injury: Autologous Genetically-Enriched Leucoconcentrate Integrated with Epidural Electrical Stimulation Open
The contemporary strategy for spinal cord injury (SCI) therapy aims to combine multiple approaches to control pathogenic mechanisms of neurodegeneration and stimulate neuroregeneration. In this study, a novel regenerative approach using an…
Evaluation of Direct and Cell-Mediated Lactoferrin Gene Therapy for the Maxillofacial Area Abscesses in Rats Open
Resistance to antibacterial therapy requires the discovery of new methods for the treatment of infectious diseases. Lactoferrin (LTF) is a well-known naïve first-line defense protein. In the present study, we suggested the use of an adenov…
Epidural Stimulation Combined with Triple Gene Therapy for Spinal Cord Injury Treatment Open
The translation of new therapies for spinal cord injury to clinical trials can be facilitated with large animal models close in morpho-physiological scale to humans. Here, we report functional restoration and morphological reorganization a…
Combined Supra- and Sub-Lesional Epidural Electrical Stimulation for Restoration of the Motor Functions after Spinal Cord Injury in Mini Pigs Open
This study evaluates the effect of combined epidural electrical stimulation (EES) applied above (C5) and below (L2) the spinal cord injury (SCI) at T8–9 combined with motor training on the restoration of sensorimotor function in mini pigs.…
View article: Preventive Triple Gene Therapy Reduces the Negative Consequences of Ischemia-Induced Brain Injury after Modelling Stroke in a Rat
Preventive Triple Gene Therapy Reduces the Negative Consequences of Ischemia-Induced Brain Injury after Modelling Stroke in a Rat Open
Currently, the main fundamental and clinical interest for stroke therapy is focused on developing a neuroprotective treatment of a penumbra region within the therapeutic window. The development of treatments for ischemic stroke in at-risk …
Gene-modified leucoconcentrate for personalized ex vivo gene therapy in a mini pig model of moderate spinal cord injury Open
We previously demonstrated that gene-modified umbilical cord blood mononuclear cells overexpressing a combination of recombinant neurotrophic factors are a promising therapeutic approach for cell-mediated gene therapy for neurodegenerative…
Neuroglia in rat spinal cord contusion injury with cell-mediated delivery of a combination of VEGF165, GDNF, and NCAM1 genes in combination with epidural electrical stimulation Open
Neural networks disturbed due to spinal cord injury are capable to restore that is largely determined by post-traumatic remodeling. It is known that information exchange between neurons is carried out by electrical impulse, which ensures t…
View article: Triple-Gene Therapy for Stroke: A Proof-of-Concept in Vivo Study in Rats
Triple-Gene Therapy for Stroke: A Proof-of-Concept in Vivo Study in Rats Open
Natural brain repair after stroke is extremely limited, and current therapeutic options are even more scarce with no clinical break-through in sight. Despite restricted regeneration in the central nervous system, we have previously proved …