Vítor C. Sousa
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View article: Ionic liquid-based nanoemulsion as a promising strategy to enhance the bioaccessibility of β-carotene obtained from Dunaliella salina
Ionic liquid-based nanoemulsion as a promising strategy to enhance the bioaccessibility of β-carotene obtained from Dunaliella salina Open
View article: Genetic variance in spider mites reveals trade-offs: lines with higher growth rate suffer stronger competition but exert stronger reproductive interference
Genetic variance in spider mites reveals trade-offs: lines with higher growth rate suffer stronger competition but exert stronger reproductive interference Open
Within-species individual variation has been gaining increased attention in ecological studies of species interactions. Yet, these studies rarely consider genetic variation and potential genetic correlations among traits. Moreover, traits …
View article: Assessment of the Critical Thermal Maximum (CTmax) of Iberian chubs v1
Assessment of the Critical Thermal Maximum (CTmax) of Iberian chubs v1 Open
The upper thermal tolerance of organisms is usually reported as the Critical Thermal Maximum, or CTmax. Here, we describe an adapted protocol to assess the CTmax in the Iberian freshwater fish of the genus Squalius, commonly known as Iberi…
View article: Dating and quantifying past gene flow due to hybridization events in Iberian chubs
Dating and quantifying past gene flow due to hybridization events in Iberian chubs Open
Hybridization - the successful reproductive cross between individuals from different species - is increasingly recognized as a relatively common phenomenon in nature, mostly due to the increasing availability of genomic data for many organ…
View article: Interactions between mechanisms of reproductive isolation
Interactions between mechanisms of reproductive isolation Open
Speciation is responsible for the diversity of species observed today and corresponds to the build-up of reproductive isolation between populations. Reproductive isolation can be generated by different mechanisms that have been extensively…
View article: A new species of the genus Squalius (Leuciscidae, Actinopterygii) from the Sado River basin in Portugal
A new species of the genus Squalius (Leuciscidae, Actinopterygii) from the Sado River basin in Portugal Open
A new species, Squalius caetobrigus sp. nov., is described based on morphological and genetic traits. Squalius caetobrigus sp. nov. can be distinguished from other Squalius species through a combination of morphometric, meristic and geneti…
View article: Building a Portuguese coalition for biodiversity genomics
Building a Portuguese coalition for biodiversity genomics Open
The diverse physiography of the Portuguese land and marine territory, spanning from continental Europe to the Atlantic archipelagos, has made it an important repository of biodiversity throughout the Pleistocene glacial cycles, leading to …
View article: Quick protocol for protein extraction from adherent fish-derived fibroblasts v1
Quick protocol for protein extraction from adherent fish-derived fibroblasts v1 Open
We present a rapid and efficient protocol for extracting total proteins from adherent fish-derived fibroblasts, specifically optimized for applications in Western blotting and mass spectrometry. The method involves directly dissociating ce…
View article: Conservation Challenges Imposed by Evolutionary History and Habitat Suitability Shifts of Endangered Freshwater Mussels under a Global Climate Change Scenario
Conservation Challenges Imposed by Evolutionary History and Habitat Suitability Shifts of Endangered Freshwater Mussels under a Global Climate Change Scenario Open
Climate change and associated shifts in temperature and precipitation patterns have become an increasing concern as drivers of ongoing biodiversity loss. The Mediterranean region is particularly vulnerable, being both a biodiversity hotspo…
View article: Towards a genome-scale metabolic model of Dunaliella salina
Towards a genome-scale metabolic model of Dunaliella salina Open
View article: Recovery and Encapsulation of Dunaliella Salina B-Carotene Through a Novel Sustainable Approach: Sequential Application of an Ionic Liquid as Naturally-Derived Solvent and Emulsifier
Recovery and Encapsulation of Dunaliella Salina B-Carotene Through a Novel Sustainable Approach: Sequential Application of an Ionic Liquid as Naturally-Derived Solvent and Emulsifier Open
View article: Competition for food affects the strength of reproductive interference and its consequences for species coexistence.
Competition for food affects the strength of reproductive interference and its consequences for species coexistence. Open
Peer reviewed
View article: Competition for food affects the strength of reproductive interference and its consequences for species coexistence.
Competition for food affects the strength of reproductive interference and its consequences for species coexistence. Open
View article: Competition for food affects the strength of reproductive interference and its consequences for species coexistence
Competition for food affects the strength of reproductive interference and its consequences for species coexistence Open
Competition for food and reproductive interference (negative interspecific sexual interactions) have been identified as major drivers of species exclusion. Still, how these biotic interactions jointly determine competitive dominance remain…
View article: Parallel evolution of local adaptation in<i>Littorina saxatilis</i>inferred with whole-genome pool-seq data
Parallel evolution of local adaptation in<i>Littorina saxatilis</i>inferred with whole-genome pool-seq data Open
Parallel evolution of phenotypic divergence offers compelling evidence supporting the influence of natural selection on the divergence process. However, the existence of ecotypes adapted to different habitats in separate locations is not a…
View article: European Reference Genome Atlas Community - Phase 1 Members - 2020-2023
European Reference Genome Atlas Community - Phase 1 Members - 2020-2023 Open
This dataset presents a comprehensive list of members registered as part of the European Reference Genome Atlas (ERGA, erga-biodiversity.eu) Community during ERGA Phase 1, which spanned from 2020 to 2023. The dataset includes information o…
View article: European Reference Genome Atlas Community - Phase 1 Members - 2020-2023
European Reference Genome Atlas Community - Phase 1 Members - 2020-2023 Open
This dataset presents a comprehensive list of members registered as part of the European Reference Genome Atlas (ERGA, erga-biodiversity.eu) Community during ERGA Phase 1, which spanned from 2020 to 2023. The dataset includes information o…
View article: poolHelper: an R package to help in designing Pool-Seq studies
poolHelper: an R package to help in designing Pool-Seq studies Open
Next-generation sequencing of pooled samples (Pool-seq) is an important tool in population genomics and molecular ecology. In Pool-seq, the relative number of reads with an allele reflects the allele frequencies in the sample. However, une…
View article: Faster‐haplodiploid evolution under divergence‐with‐gene‐flow: Simulations and empirical data from pine‐feeding hymenopterans
Faster‐haplodiploid evolution under divergence‐with‐gene‐flow: Simulations and empirical data from pine‐feeding hymenopterans Open
Although haplodiploidy is widespread in nature, the evolutionary consequences of this mode of reproduction are not well characterized. Here, we examine how genome‐wide hemizygosity and a lack of recombination in haploid males affects genom…
View article: Rapid and repeatable genome evolution across three hybrid ant populations
Rapid and repeatable genome evolution across three hybrid ant populations Open
Hybridization is frequent in the wild but it is unclear whether admixture events lead to predictable outcomes and if so, at what timescale. We show that selection led to correlated sorting of genetic variation in less than 50 generations i…
View article: Genomic data and multi-species demographic modelling uncover past hybridization between currently allopatric freshwater species
Genomic data and multi-species demographic modelling uncover past hybridization between currently allopatric freshwater species Open
View article: The genomic history of the Aegean palatial civilizations
The genomic history of the Aegean palatial civilizations Open
View article: Faster-haplodiploid evolution under divergence-with-gene-flow: simulations and empirical data from pine-feeding hymenopterans
Faster-haplodiploid evolution under divergence-with-gene-flow: simulations and empirical data from pine-feeding hymenopterans Open
Although haplodiploidy is widespread in nature, the evolutionary consequences of this sex determination mechanism are not well characterized. Here, we examine how genome-wide hemizygosity and a lack of recombination in haploid males affect…
View article: Whole-genome analysis of multiple wood ant population pairs supports similar speciation histories, but different degrees of gene flow, across their European range
Whole-genome analysis of multiple wood ant population pairs supports similar speciation histories, but different degrees of gene flow, across their European range Open
The application of demographic history modeling and inference to the study of divergence between species is becoming a cornerstone of speciation genomics. The demographic history is usually reconstructed by analysing a single population fr…
View article: Adaptation and convergence in circadian-related genes in Iberian freshwater fish
Adaptation and convergence in circadian-related genes in Iberian freshwater fish Open
BackgroundThe circadian clock is a biological timing system that improves the ability of organisms to deal with environmental fluctuations. At the molecular level it consists of a network of transcription-translation feedback loops, involv…
View article: Additional file 12 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish
Additional file 12 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish Open
Additional file 12: Table S11. Number of sites in coding proteins for genes of the negative loop (cry and per) and of the positive loop (bmal and clock) of circadian-related genes.
View article: Additional file 13 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish
Additional file 13 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish Open
Additional file 13: Table S12. List of primer pairs and respective sequences used in PCR to (re)sequence circadian-related genes with Sanger method in Squalius species.
View article: Additional file 6 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish
Additional file 6 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish Open
Additional file 6: Table S5. Summary of sites detected to be under positive selection using MEME and FUBAR methods, and corresponding changes in amino acid and predicted impacts.
View article: Additional file 14 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish
Additional file 14 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish Open
Additional file 14: Table S13. PCR conditions for each pair of primers (Additional file 13: Table S12) used in amplification of circadian-related genes.
View article: Additional file 8 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish
Additional file 8 of Adaptation and convergence in circadian‐related genes in Iberian freshwater fish Open
Additional file 8: Table S7. Summary of sites with evidence of increased dN/dS ratios (estimated β) on Squalius torgalensis and S. aradensis clade (foreground) or in S. carolitertii, S. pyrenaicus clade (background), corresponding changes …