Věra Sovková
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View article: Stimulation of MSCs Osteogenic Differentiation on a Micro/ Nanofibrous Scaffold Using Mechanical Stimulation in the Perfusion Bioreactor
Stimulation of MSCs Osteogenic Differentiation on a Micro/ Nanofibrous Scaffold Using Mechanical Stimulation in the Perfusion Bioreactor Open
In tissue engineering, the 3D constructs seeded with cells tested in vitro in static conditions suffer from poor cellular penetration and non-homogenous matrix deposition, due to the insufficient flow of nutrients, gases and signal molecul…
View article: The Effect of Osteoblast Isolation Methods from Adult Rats on Osteoclastogenesis in Co-Cultures
The Effect of Osteoblast Isolation Methods from Adult Rats on Osteoclastogenesis in Co-Cultures Open
Co-cultures of osteoblasts and osteoclasts are on the rise because they enable a more complex study. Diseases such as osteoporosis are related to a higher age. Thus, cell isolation from adult individuals is necessary. Osteoblasts can be is…
View article: Cellular Response to Individual Components of the Platelet Concentrate
Cellular Response to Individual Components of the Platelet Concentrate Open
Platelet concentrates and especially their further product platelet lysate, are widely used as a replacement for cell culturing. Platelets contain a broad spectrum of growth factors and bioactive molecules that affect cellular fate. Howeve…
View article: FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends
FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends Open
Tissue engineering is a current trend in the regenerative medicine putting pressure on scientists to develop highly functional materials and methods for scaffolds’ preparation. In this paper, the calibrated filaments for Fused Deposition M…
View article: A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment
A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment Open
Vitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. …
View article: FUNCTIONALIZATION OF POLYMERIC NANOFIBERS USING PLATELETS FOR MELANOCYTE CULTURE
FUNCTIONALIZATION OF POLYMERIC NANOFIBERS USING PLATELETS FOR MELANOCYTE CULTURE Open
Tissue engineering is an interdisciplinary field that uses a combination of cells, suitable biomaterials and bioactive molecules to engineer the desired tissue and restore lost function. These principles have quickly begun to spread to the…
View article: Mesenchymal stem cell interaction with Ti<sub>6</sub>Al<sub>4</sub>V alloy pre-exposed to simulated body fluid
Mesenchymal stem cell interaction with Ti<sub>6</sub>Al<sub>4</sub>V alloy pre-exposed to simulated body fluid Open
Titanium and its alloys are widely used for substitution of hard tissues, especially in orthopaedic and dental surgery.
View article: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface
Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface Open
Nanostructured cpTi surfaces affected Saos-2 cell adhesion, proliferation, and osteogenic differentiation as well as the nanomechanical properties of the surface.
View article: PVA and PCL nanofibers are suitable for tissue covering and regeneration
PVA and PCL nanofibers are suitable for tissue covering and regeneration Open
The aim of the study was to evaluate the safety and efficacy of a new therapeutic approach to skin defects resulting from split thickness grafting. Within the study, nanofiber-based dressings fabricated using polyvinyl alcohol (PVA) and po…
View article: A polypropylene mesh modified with poly-&epsilon;-caprolactone nanofibers in hernia repair: large animal experiment
A polypropylene mesh modified with poly-ε-caprolactone nanofibers in hernia repair: large animal experiment Open
We believe that PCL nanofibers on their own can cause enough fibroplasia to be used as a separate material without the polypropylene base, thus avoiding potential adverse effects caused by any added substances.
View article: A polypropylene mesh modified with poly-ε-caprolactone nanofibers in hernia repair: large animal experiment
A polypropylene mesh modified with poly-ε-caprolactone nanofibers in hernia repair: large animal experiment Open
Barbora East,1,2 Martin Plencner,3,4 Martin Kralovic,1,3,5 Michala Rampichova,3 Vera Sovkova,1,3,5 Karolina Vocetkova,1,3,5 Martin Otahal,6,7 Zbynek Tonar,8,9 Yaroslav Kolinko,8,9 Evzen Amler,1,3,5 Jiri Hoch1,10 1Second Medical Faculty, Ch…
View article: Polycaprolactone nanofiber mesh with adhered liposomes as a simple delivery system for bioactive growth factors
Polycaprolactone nanofiber mesh with adhered liposomes as a simple delivery system for bioactive growth factors Open
Polycaprolactone (PCL) nanofibers were prepared using electrospinning and functionalized with liposomes containing growth factors by simple surface adhesion. A tight interaction between the liposomes and PCL nanofibers has been clearly dem…
View article: Osteoinductive 3D scaffolds prepared by blend centrifugal spinning for long-term delivery of osteogenic supplements
Osteoinductive 3D scaffolds prepared by blend centrifugal spinning for long-term delivery of osteogenic supplements Open
Bone regeneration is a long-term process requiring proper scaffolding and drug delivery systems.
View article: Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides
Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides Open
Objectives Bioactive peptides derived from receptor binding motifs of native proteins are a potent source of bioactive molecules that can induce signalling pathways. These peptides could substitute for osteogenesis promoting supplements. T…
View article: FUNCTIONALIZATION OF 3D FIBROUS SCAFFOLDS PREPARED USING CENTRIFUGAL SPINNING WITH LIPOSOMES AS A SIMPLE DRUG DELIVERY SYSTEM
FUNCTIONALIZATION OF 3D FIBROUS SCAFFOLDS PREPARED USING CENTRIFUGAL SPINNING WITH LIPOSOMES AS A SIMPLE DRUG DELIVERY SYSTEM Open
3D materials supporting cell adhesion, infiltration and proliferation are crucial for bone tissue engineering. In the current study we combined PCL fibers prepared using centrifugal spinning with adhered liposomes filled with platelet lysa…
View article: Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers
Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers Open
Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum (FBS) replacement for skin cell culture. The cytokine conten…
View article: Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers
Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers Open
Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum (FBS) replacement for skin cell culture. The cytokine conten…
View article: A comparison of high throughput core–shell 2D electrospinning and 3D centrifugal spinning techniques to produce platelet lyophilisate-loaded fibrous scaffolds and their effects on skin cells
A comparison of high throughput core–shell 2D electrospinning and 3D centrifugal spinning techniques to produce platelet lyophilisate-loaded fibrous scaffolds and their effects on skin cells Open
Nanofibres enriched with bioactive molecules, as actively acting scaffolds, play an important role in tissue engineering.
View article: Platelet-functionalized three-dimensional poly-&epsilon;-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors
Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors Open
Bone and cartilage are tissues of a three-dimensional (3D) nature. Therefore, scaffolds for their regeneration should support cell infiltration and growth in all 3 dimensions. To fulfill such a requirement, the materials should possess lar…
View article: Emulsion centrifugal spinning for production of 3D drug releasing nanofibres with core/shell structure
Emulsion centrifugal spinning for production of 3D drug releasing nanofibres with core/shell structure Open
Herein we describe the core/shell centrifugal spinning process to deliver susceptible bioactive molecules.
View article: Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells
Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells Open
Objectives Faulty wound healing is a global healthcare problem. Chronic wounds are generally characterized by a reduction in availability of growth factors. New strategies are being developed to deliver growth factors more effectively. Met…