Viktoriia E. Baksheeva
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View article: NanoDSF Screening for Anti-tubulin Agents Uncovers New Structure–Activity Insights
NanoDSF Screening for Anti-tubulin Agents Uncovers New Structure–Activity Insights Open
Microtubule targeting agents (MTAs) constitute a vital category of tubulin-binding compounds deployed across anticancer therapies. Despite the array of MTA drugs developed by pharmaceutical entities, the quest for novel efficacious molecul…
View article: A role of pigment epithelium-derived factor in zinc-mediated mechanism of neurodegeneration in glaucoma
A role of pigment epithelium-derived factor in zinc-mediated mechanism of neurodegeneration in glaucoma Open
Glaucoma is a neurodegenerative condition involving optic nerve damage and retinal ganglion cells death. Animal studies suggested that the pathway linking these events can be mediated by mobile zinc secreted into the intraretinal space and…
View article: Every Piece of the Puzzle Matters: A Novel Zinc-Binding Site in the Luminal Domain of STIM1 Drives Clustering
Every Piece of the Puzzle Matters: A Novel Zinc-Binding Site in the Luminal Domain of STIM1 Drives Clustering Open
STIM1 is pivotal in the tightly regulated mechanism controlling calcium homeostasis in the ER. It is activated by calcium dissociation from its EF-hand domain when ER calcium levels decrease, leading to its interaction with the ORAI channe…
View article: AI-Driven Plasma Denaturation Profiling for Multi-Cancer Detection
AI-Driven Plasma Denaturation Profiling for Multi-Cancer Detection Open
Many cancers cannot be detected early due to lack of effective disease biomarkers, leading to poor prognosis. We applied an existing biophysical technology nanoDSF in a novel way to answer this unmet biomedical need. We developed a breakth…
View article: Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis
Targeting Oxidative Stress and Inflammation in the Eye: Insights from a New Model of Experimental Autoimmune Uveitis Open
Autoimmune uveitis is a relapsing blind-causing ocular condition with complex pathogenesis that is not completely understood. There is a high demand for accurate animal models of experimental autoimmune uveitis (EAU) suitable for elucidati…
View article: Tear nanoDSF Denaturation Profile Is Predictive of Glaucoma
Tear nanoDSF Denaturation Profile Is Predictive of Glaucoma Open
Primary open-angle glaucoma (POAG) is a frequent blindness-causing neurodegenerative disorder characterized by optic nerve and retinal ganglion cell damage most commonly due to a chronic increase in intraocular pressure. The preservation o…
View article: Plasma nanoDSF Denaturation Profile at Baseline Is Predictive of Glioblastoma EGFR Status
Plasma nanoDSF Denaturation Profile at Baseline Is Predictive of Glioblastoma EGFR Status Open
Glioblastoma (GBM) is the most frequent and aggressive primary brain tumor in adults. Recently, we demonstrated that plasma denaturation profiles of glioblastoma patients obtained using Differential Scanning Fluorimetry can be automaticall…
View article: Redox Regulation of Signaling Complex between Caveolin-1 and Neuronal Calcium Sensor Recoverin
Redox Regulation of Signaling Complex between Caveolin-1 and Neuronal Calcium Sensor Recoverin Open
Caveolin-1 is a cholesterol-binding scaffold protein, which is localized in detergent-resistant membrane (DRM) rafts and interacts with components of signal transduction systems, including visual cascade. Among these components are neurona…
View article: Zinc Modulation of Neuronal Calcium Sensor Proteins: Three Modes of Interaction with Different Structural Outcomes
Zinc Modulation of Neuronal Calcium Sensor Proteins: Three Modes of Interaction with Different Structural Outcomes Open
Neuronal calcium sensors (NCSs) are the family of EF-hand proteins mediating Ca2+-dependent signaling pathways in healthy neurons and neurodegenerative diseases. It was hypothesized that the calcium sensor activity of NCSs can be complemen…
View article: Mechanism of Zn2+ and Ca2+ Binding to Human S100A1
Mechanism of Zn2+ and Ca2+ Binding to Human S100A1 Open
S100A1 is a member of the S100 family of small ubiquitous Ca2+-binding proteins, which participates in the regulation of cell differentiation, motility, and survival. It exists as homo- or heterodimers. S100A1 has also been shown to bind Z…
View article: Disulfide Dimerization of Neuronal Calcium Sensor-1: Implications for Zinc and Redox Signaling
Disulfide Dimerization of Neuronal Calcium Sensor-1: Implications for Zinc and Redox Signaling Open
Neuronal calcium sensor-1 (NCS-1) is a four-EF-hand ubiquitous signaling protein modulating neuronal function and survival, which participates in neurodegeneration and carcinogenesis. NCS-1 recognizes specific sites on cellular membranes a…
View article: Inflammation in Dry Eye Syndrome: Identification and Targeting of Oxylipin-Mediated Mechanisms
Inflammation in Dry Eye Syndrome: Identification and Targeting of Oxylipin-Mediated Mechanisms Open
Dry eye syndrome (DES) is characterized by decreased tear production and stability, leading to desiccating stress, inflammation and corneal damage. DES treatment may involve targeting the contributing inflammatory pathways mediated by poly…
View article: A Novel Approach to Bacterial Expression and Purification of Myristoylated Forms of Neuronal Calcium Sensor Proteins
A Novel Approach to Bacterial Expression and Purification of Myristoylated Forms of Neuronal Calcium Sensor Proteins Open
N-terminal myristoylation is a common co-and post-translational modification of numerous eukaryotic and viral proteins, which affects their interaction with lipids and partner proteins, thereby modulating various cellular processes. Among …
View article: Mechanisms and Treatment of Light-Induced Retinal Degeneration-Associated Inflammation: Insights from Biochemical Profiling of the Aqueous Humor
Mechanisms and Treatment of Light-Induced Retinal Degeneration-Associated Inflammation: Insights from Biochemical Profiling of the Aqueous Humor Open
Ocular inflammation contributes to the pathogenesis of blind-causing retinal degenerative diseases, such as age-related macular degeneration (AMD) or photic maculopathy. Here, we report on inflammatory mechanisms that are associated with r…
View article: Membrane Binding of Neuronal Calcium Sensor-1: Highly Specific Interaction with Phosphatidylinositol-3-Phosphate
Membrane Binding of Neuronal Calcium Sensor-1: Highly Specific Interaction with Phosphatidylinositol-3-Phosphate Open
Neuronal calcium sensors are a family of N-terminally myristoylated membrane-binding proteins possessing a different intracellular localization and thereby targeting unique signaling partner(s). Apart from the myristoyl group, the membrane…
View article: Alterations in proteome of human sclera associated with primary open-angle glaucoma involve proteins participating in regulation of the extracellular matrix.
Alterations in proteome of human sclera associated with primary open-angle glaucoma involve proteins participating in regulation of the extracellular matrix. Open
POAG is accompanied by alterations of the scleral proteome, which represent a novel hallmark of the disease and can reflect pathological changes in scleral biochemistry and biomechanics. The potential mechanisms underlying these changes re…
View article: Experimental Insight into the Structural and Functional Roles of the ‘Black’ and ‘Gray’ Clusters in Recoverin, a Calcium Binding Protein with Four EF-Hand Motifs
Experimental Insight into the Structural and Functional Roles of the ‘Black’ and ‘Gray’ Clusters in Recoverin, a Calcium Binding Protein with Four EF-Hand Motifs Open
Recently, we have found that calcium binding proteins of the EF-hand superfamily (i.e., a large family of proteins containing helix-loop-helix calcium binding motif or EF-hand) contain two types of conserved clusters called cluster I (‘bla…
View article: Light-Induced Thiol Oxidation of Recoverin Affects Rhodopsin Desensitization
Light-Induced Thiol Oxidation of Recoverin Affects Rhodopsin Desensitization Open
The excessive light illumination of mammalian retina is known to induce oxidative stress and photoreceptor cell death linked to progression of age-related macular degeneration. The photochemical damage of photoreceptors is suggested to occ…
View article: Inflammatory metabolites of arahidonic acid in tear fluid in UV-induced corneal damage
Inflammatory metabolites of arahidonic acid in tear fluid in UV-induced corneal damage Open
The ultraviolet (UV) B-induced damage of the eye surface of experimental animals (rabbits) includes loss of corneal epithelium, apoptosis of keratocytes and stromal edema. These changes are accompanied by clinically and histologically mani…
View article: Suppression of Light-Induced Oxidative Stress in the Retina by Mitochondria-Targeted Antioxidant
Suppression of Light-Induced Oxidative Stress in the Retina by Mitochondria-Targeted Antioxidant Open
Light-induced oxidation of lipids and proteins provokes retinal injuries and results in progression of degenerative retinal diseases, such as, for instance, iatrogenic photic maculopathies. Having accumulated over years retinal injuries co…
View article: Functional Status of Neuronal Calcium Sensor-1 Is Modulated by Zinc Binding
Functional Status of Neuronal Calcium Sensor-1 Is Modulated by Zinc Binding Open
Neuronal calcium sensor-1 (NCS-1) protein is abundantly expressed in the central nervous system and retinal neurons, where it regulates many vital processes such as synaptic transmission. It coordinates three calcium ions by EF-hands 2-4, …
View article: MITOCHONDRIALLY ADDRESSED ANTI-OXIDANT THERAPY FOR PREVENTION OF PERI-OPERATIVE CORNEAL EROSION
MITOCHONDRIALLY ADDRESSED ANTI-OXIDANT THERAPY FOR PREVENTION OF PERI-OPERATIVE CORNEAL EROSION Open
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View article: Mitochondria‐Targeted Antioxidant SkQ1 Prevents Anesthesia‐Induced Dry Eye Syndrome
Mitochondria‐Targeted Antioxidant SkQ1 Prevents Anesthesia‐Induced Dry Eye Syndrome Open
Dry eye syndrome (DES) is an age‐related condition increasingly detected in younger people of risk groups, including patients who underwent ocular surgery or long‐term general anesthesia. Being a multifactorial disease, it is characterized…
View article: Interleukin-11 binds specific EF-hand proteins via their conserved structural motifs
Interleukin-11 binds specific EF-hand proteins via their conserved structural motifs Open
Interleukin-11 (IL-11) is a hematopoietic cytokine engaged in numerous biological processes and validated as a target for treatment of various cancers. IL-11 contains intrinsically disordered regions that might recognize multiple targets. …
View article: Mechanisms of perioperative corneal abrasions: alterations in tear film proteome
Mechanisms of perioperative corneal abrasions: alterations in tear film proteome Open
Perioperative corneal abrasion is an ophthalmic complication commonly found in patients underwent general anesthesia. In this study, correlations between development of corneal injury and proteomic changes in tear film during general anest…