Vincent Allain
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View article: Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells
Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells Open
Primary human myeloid cells are promising candidates for immunotherapy, yet efficient and scalable technologies for genetic engineering and screening in these cells are limited. Here we present a virus-like particle (VLP)-based toolkit tha…
View article: Harnessing <scp>HLA</scp> Divergence for Improved Donor Selection in Haploidentical Haematopoietic Stem Cell Transplantation
Harnessing <span>HLA</span> Divergence for Improved Donor Selection in Haploidentical Haematopoietic Stem Cell Transplantation Open
Haploidentical haematopoietic stem cell transplantation (haplo‐HSCT) with post‐transplant cyclophosphamide (PTCy)‐based graft‐versus‐host disease (GVHD) prophylaxis is commonly used in patients without a fully matched donor. HLA evolutiona…
View article: Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function
Site-specific genome engineering of primary human natural killer cells for programmable anti-tumor function Open
Natural killer (NK) cells are emerging as a promising platform for engineered adoptive cell therapies. However, gene editing in NK cells remains challenging, and more effective strategies are needed. Here, we established a robust, feeder-f…
View article: Donor HLA-DQ genetic and functional divergence affect the control of BK polyoma virus infection after kidney transplantation
Donor HLA-DQ genetic and functional divergence affect the control of BK polyoma virus infection after kidney transplantation Open
BK polyomavirus (BKPyV) infection remains a major concern after kidney transplantation, increasing the risk of graft loss in the absence of specific antiviral agent now available. Here, we investigated the impact of HLA diversity on the co…
View article: SYK negatively regulates ITAM-mediated human NK cell signaling and CD19-CAR NK cell efficacy
SYK negatively regulates ITAM-mediated human NK cell signaling and CD19-CAR NK cell efficacy Open
Natural killer (NK) cells express activating receptors that signal through ITAM (immunoreceptor tyrosine-based activation motif)-bearing adapter proteins. The phosphorylation of each ITAM creates binding sites for SYK and ZAP70 protein tyr…
View article: Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells
Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells Open
Adoptive chimeric antigen receptor T-cell (CAR-T) therapy is transformative and approved for hematologic malignancies. It is also being developed for the treatment of solid tumors, autoimmune disorders, heart disease, and aging. Despite un…
View article: HLA evolutionary divergence score after donor lymphocyte infusion following allogeneic hematopoietic stem cell transplantation
HLA evolutionary divergence score after donor lymphocyte infusion following allogeneic hematopoietic stem cell transplantation Open
Donor lymphocyte infusion (DLI) prevents acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) relapses following hematopoietic stem cell transplantation. Given the life‐threatening toxicities such as graft versus host disease (GV…
View article: Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells
Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells Open
Adoptive chimeric antigen receptor T-cell (CAR-T) therapy is transformative and approved for hematologic malignancies. It is also being developed for the treatment of solid tumors, autoimmune disorders, heart disease, and aging. Despite un…
View article: Neurofilament light chain levels as an early predictive biomarker of neurotoxicity after CAR T-cell therapy
Neurofilament light chain levels as an early predictive biomarker of neurotoxicity after CAR T-cell therapy Open
Immune effector cell-associated neurotoxicity syndrome (ICANS) remains a significant cause of morbidity associated with CD19-targeted chimeric antigen receptor (CAR) T-cell therapy. Early prediction of patients who will develop ICANS would…
View article: Spleen Tyrosine Kinase (SYK) negatively regulates ITAM-mediated human NK cell signaling and CD19-CAR NK cell efficacy
Spleen Tyrosine Kinase (SYK) negatively regulates ITAM-mediated human NK cell signaling and CD19-CAR NK cell efficacy Open
NK cells express activating receptors that signal through ITAM-bearing adapter proteins. The phosphorylation of each ITAM creates binding sites for SYK and ZAP70 protein tyrosine kinases to propagate downstream signaling including the indu…
View article: Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells
Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells Open
Adoptive chimeric antigen receptor T-cell (CAR-T) therapy is transformative and approved for hematologic malignancies. It is also being developed for the treatment of solid tumors, autoimmune disorders, heart disease, and aging. Despite un…
View article: Ultra-high efficiency T cell reprogramming at multiple loci with SEED-Selection
Ultra-high efficiency T cell reprogramming at multiple loci with SEED-Selection Open
Multiplexed reprogramming of T cell specificity and function can generate powerful next-generation cellular therapies. However, current manufacturing methods produce heterogenous mixtures of partially engineered cells. Here, we develop a o…
View article: Germline HLA-DQ genotype influences response to CAR T-cell therapy in patients with large B-cell lymphoma
Germline HLA-DQ genotype influences response to CAR T-cell therapy in patients with large B-cell lymphoma Open
CD19-directed chimeric antigen receptor (CAR) T cells have greatly improved the prognosis of relapsed/refractory large B-cell lymphoma (rrLBCL), yet treatment failure occurs in more than half of patients, usually in the first 3 months afte…
View article: A major role for CD4+ T cells in driving cytokine release syndrome during CAR T cell therapy
A major role for CD4+ T cells in driving cytokine release syndrome during CAR T cell therapy Open
Anti-CD19 chimeric antigen receptor (CAR) T cell therapy represents a breakthrough for the treatment of B cell malignancies. Yet, it can lead to severe adverse events, including cytokine release syndrome (CRS), which may require urgent cli…
View article: HLA-DP diversity is associated with improved response to SARS-Cov-2 vaccine in hematopoietic stem cell transplant recipients
HLA-DP diversity is associated with improved response to SARS-Cov-2 vaccine in hematopoietic stem cell transplant recipients Open
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients show lower humoral vaccine responsiveness than immunocompetent individuals. HLA diversity, measured by the HLA evolutionary divergence (HED) metrics, reflects the di…
View article: IPSS-M in myelodysplastic neoplasms arising from aplastic anemia and paroxysmal nocturnal hemoglobinuria
IPSS-M in myelodysplastic neoplasms arising from aplastic anemia and paroxysmal nocturnal hemoglobinuria Open
International audience
View article: An evolved AAV variant enables efficient genetic engineering of murine T cells
An evolved AAV variant enables efficient genetic engineering of murine T cells Open
Precise targeting of large transgenes to T cells using homology-directed repair has been transformative for adoptive cell therapies and T cell biology. Delivery of DNA templates via adeno-associated virus (AAV) has greatly improved knockin…
View article: Clinical and Molecular Determinants of Clonal Evolution in Aplastic Anemia and Paroxysmal Nocturnal Hemoglobinuria
Clinical and Molecular Determinants of Clonal Evolution in Aplastic Anemia and Paroxysmal Nocturnal Hemoglobinuria Open
PURPOSE Secondary myeloid neoplasms (sMNs) remain the most serious long-term complications in patients with aplastic anemia (AA) and paroxysmal nocturnal hemoglobinuria (PNH). However, sMNs lack specific predictors, dedicated surveillance …
View article: Impact of HLA divergence on humoral response to SARS-CoV-2 and HBV vaccines in the liver transplantation setting
Impact of HLA divergence on humoral response to SARS-CoV-2 and HBV vaccines in the liver transplantation setting Open
Background Organ transplant recipients are at high risk of viral infections but show lower humoral vaccine responsiveness than immunocompetent individuals. HLA evolutionary divergence (HED) quantifies the sequence differences between homol…
View article: Real-world characteristics of T-cell apheresis and clinical response to tisagenlecleucel in B-cell lymphoma
Real-world characteristics of T-cell apheresis and clinical response to tisagenlecleucel in B-cell lymphoma Open
International audience
View article: Donor HLA class 1 evolutionary divergence is a major predictor of liver allograft rejection: a retrospective cohort study
Donor HLA class 1 evolutionary divergence is a major predictor of liver allograft rejection: a retrospective cohort study Open
Background Recognition of donor antigens by the recipient’s immune system leads to allograft rejection. HLA evolutionary divergence (HED) between an individual’s HLA alleles is a continuous metric that quantifies the differences between ea…