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View article: The role of FoxA, FiuA, and FpvB in iron acquisition via hydroxamate-type siderophores in Pseudomonas aeruginosa
The role of FoxA, FiuA, and FpvB in iron acquisition via hydroxamate-type siderophores in Pseudomonas aeruginosa Open
Siderophores are specialized molecules produced by bacteria and fungi to scavenge iron, a crucial nutrient for growth and metabolism. Catecholate-type siderophores are mainly produced by bacteria, while hydroxamates are mostly from fungi. …
View article: Trojan Horse Siderophore Conjugates Induce <i>Pseudomonas aeruginosa</i> Suicide and Qualify the TonB Protein as a Novel Antibiotic Target
Trojan Horse Siderophore Conjugates Induce <i>Pseudomonas aeruginosa</i> Suicide and Qualify the TonB Protein as a Novel Antibiotic Target Open
Rising infection rates with multidrug-resistant pathogens calls for antibiotics with novel modes of action. Herein, we identify the inner membrane protein TonB, a motor of active uptake in Gram-negative bacteria, as a novel target in antim…
View article: Trojan Horse siderophore conjugates induce P. aeruginosa suicide and qualify the TonB protein as a novel antibiotic target
Trojan Horse siderophore conjugates induce P. aeruginosa suicide and qualify the TonB protein as a novel antibiotic target Open
Rising infection rates with multidrug-resistant bacterial pathogens such as Pseudomonas combined with a shallow antibiotic pipeline urgently call for antibiotics with novel modes of action. Herein, we identify the inner membrane protein To…
View article: Uptake Mechanisms and Regulatory Responses to MECAM- and DOTAM-Based Artificial Siderophores and Their Antibiotic Conjugates in <i>Pseudomonas aeruginosa</i>
Uptake Mechanisms and Regulatory Responses to MECAM- and DOTAM-Based Artificial Siderophores and Their Antibiotic Conjugates in <i>Pseudomonas aeruginosa</i> Open
The development of new antibiotics against Gram-negative bacteria has to deal with the low permeability of the outer membrane. This obstacle can be overcome by utilizing siderophore-dependent iron uptake pathways as entrance routes for ant…
View article: How the Presence of Hemin Affects the Expression of the Different Iron Uptake Pathways in <i>Pseudomonas aeruginosa</i> Cells
How the Presence of Hemin Affects the Expression of the Different Iron Uptake Pathways in <i>Pseudomonas aeruginosa</i> Cells Open
Iron is an essential nutriment for almost all organisms, but this metal is poorly bioavailable. During infection, bacteria access iron from the host by importing either iron or heme. Pseudomonas aeruginosa, a gram-negative pathogen,…
View article: A role for <scp>PchHI</scp> as the <scp>ABC</scp> transporter in iron acquisition by the siderophore pyochelin in <i>Pseudomonas aeruginosa</i>
A role for <span>PchHI</span> as the <span>ABC</span> transporter in iron acquisition by the siderophore pyochelin in <i>Pseudomonas aeruginosa</i> Open
Summary Iron is an essential nutrient for bacterial growth but poorly bioavailable. Bacteria scavenge ferric iron by synthesizing and secreting siderophores, small compounds with a high affinity for iron. Pyochelin (PCH) is one of the two …
View article: Structural insights into a novel family of integral membrane siderophore reductases
Structural insights into a novel family of integral membrane siderophore reductases Open
Significance Secretion of siderophores allows most microbes to assimilate ferric ions into their biological processes. Siderophores must be taken up into the cells, and chelated iron must be released. Here, we present the structure of an i…
View article: Structural insights into a novel family of integral membrane siderophore reductases
Structural insights into a novel family of integral membrane siderophore reductases Open
Gram-negative bacteria take up the essential ion Fe 3+ as ferric-siderophore complexes through their outer membrane using TonB-dependent transporters. However, the subsequent route through the inner membrane differs across many bacterial s…
View article: Opportunistic use of catecholamine neurotransmitters as siderophores to access iron by <scp> <i>Pseudomonas aeruginosa</i> </scp>
Opportunistic use of catecholamine neurotransmitters as siderophores to access iron by <span> <i>Pseudomonas aeruginosa</i> </span> Open
Summary Iron is an essential nutrient for bacterial growth and the cause of a fierce battle between the pathogen and host during infection. Bacteria have developed several strategies to access iron from the host, the most common being the …
View article: Hemeprotein Tpx1 interacts with cell‐surface heme transporter Str3 in <i>Schizosaccharomyces pombe</i>
Hemeprotein Tpx1 interacts with cell‐surface heme transporter Str3 in <i>Schizosaccharomyces pombe</i> Open
Str3 is a transmembrane protein that mediates low‐affinity heme uptake in Schizosaccharomyces pombe . Under iron‐limiting conditions, Str3 remains at the cell surface in the presence of increasing hemin concentrations. Using a proximity‐de…
View article: Nocardamine-Dependent Iron Uptake in <i>Pseudomonas aeruginosa</i>: Exclusive Involvement of the FoxA Outer Membrane Transporter
Nocardamine-Dependent Iron Uptake in <i>Pseudomonas aeruginosa</i>: Exclusive Involvement of the FoxA Outer Membrane Transporter Open
Iron is a key nutrient for almost all living organisms. Paradoxically, it is poorly soluble and consequently poorly bioavailable. Bacteria have thus developed multiple strategies to access this metal. One of the most common consists of the…
View article: FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria. Open
Protein-protein interactions (PPIs) control various key processes in cells. Fluorescence lifetime imaging microscopy (FLIM) combined with Förster resonance energy transfer (FRET) provide accurate information about PPIs in live cells. FLIM-…
View article: Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms
Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms Open
View article: Opportunistic Use by <i>Pseudomonas</i> Aeruginosa of Catecholamine Neurotransmitters as Siderophore to Access Iron
Opportunistic Use by <i>Pseudomonas</i> Aeruginosa of Catecholamine Neurotransmitters as Siderophore to Access Iron Open
View article: A role for the transcription factor Mca1 in activating the meiosis-specific copper transporter Mfc1
A role for the transcription factor Mca1 in activating the meiosis-specific copper transporter Mfc1 Open
When reproduction in fungi takes place by sexual means, meiosis enables the formation of haploid spores from diploid precursor cells. Copper is required for completion of meiosis in Schizosaccharomyces pombe. During the meiotic program, ge…
View article: The major facilitator transporter Str3 is required for low-affinity heme acquisition in Schizosaccharomyces pombe
The major facilitator transporter Str3 is required for low-affinity heme acquisition in Schizosaccharomyces pombe Open
In the fission yeast Schizosaccharomyces pombe, acquisition of exogenous heme is largely mediated by the cell membrane-associated Shu1. Here, we report that Str3, a member of the major facilitator superfamily of transporters, promot…
View article: Caractérisation des protéines Str3 et Tpx1 dans l’homéostasie de l’hème chez la levure Schizosaccharomyces pombe.
Caractérisation des protéines Str3 et Tpx1 dans l’homéostasie de l’hème chez la levure Schizosaccharomyces pombe. Open
La molécule d’hème est un groupement prosthétique qui est requis par de nombreuses
\nprotéines impliquées dans des processus cellulaires vitaux. En excès, la molécule d’hème
\nlibre est toxique car elle peut facilement causer des dommages …
View article: Cell-surface copper transporters and superoxide dismutase 1 are essential for outgrowth during fungal spore germination
Cell-surface copper transporters and superoxide dismutase 1 are essential for outgrowth during fungal spore germination Open
During fungal spore germination, a resting spore returns to a conventional mode of cell division and resumes vegetative growth, but the requirements for spore germination are incompletely understood. Here, we show that copper is essential …
View article: Heme Assimilation in Schizosaccharomyces pombe Requires Cell-surface-anchored Protein Shu1 and Vacuolar Transporter Abc3
Heme Assimilation in Schizosaccharomyces pombe Requires Cell-surface-anchored Protein Shu1 and Vacuolar Transporter Abc3 Open
View article: Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells
Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells Open
Meiosis is essential for sexually reproducing organisms, including the fission yeast Schizosaccharomyces pombe. In meiosis, chromosomes replicate once in a diploid precursor cell (zygote), and then segregate twice to generate four haploid …
View article: An Antisense RNA-mediated Mechanism Eliminates a Meiosis-specific Copper-regulated Transcript in Mitotic Cells
An Antisense RNA-mediated Mechanism Eliminates a Meiosis-specific Copper-regulated Transcript in Mitotic Cells Open
Sense and antisense transcripts produced from convergent gene pairs could interfere with the expression of either partner gene. In Schizosaccharomyces pombe, we found that the iss1(+) gene produces two transcript isoforms, including a long…