Vladimír Pevala
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View article: A fully automated morphological analysis of yeast mitochondria from wide-field fluorescence images
A fully automated morphological analysis of yeast mitochondria from wide-field fluorescence images Open
View article: Polyphosphate and tyrosine phosphorylation in the N-terminal domain of the human mitochondrial Lon protease disrupts its functions
Polyphosphate and tyrosine phosphorylation in the N-terminal domain of the human mitochondrial Lon protease disrupts its functions Open
View article: Cost-Effective Protein Production in CHO Cells Following Polyethylenimine-Mediated Gene Delivery Showcased by the Production and Crystallization of Antibody Fabs
Cost-Effective Protein Production in CHO Cells Following Polyethylenimine-Mediated Gene Delivery Showcased by the Production and Crystallization of Antibody Fabs Open
Laboratory production of recombinant mammalian proteins, particularly antibodies, requires an expression pipeline assuring sufficient yield and correct folding with appropriate posttranslational modifications. Transient gene expression (TG…
View article: Mitochondrial Processing Peptidases—Structure, Function and the Role in Human Diseases
Mitochondrial Processing Peptidases—Structure, Function and the Role in Human Diseases Open
Mitochondrial proteins are encoded by both nuclear and mitochondrial DNA. While some of the essential subunits of the oxidative phosphorylation (OXPHOS) complexes responsible for cellular ATP production are synthesized directly in the mito…
View article: The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids
The yeast mitochondrial succinylome: Implications for regulation of mitochondrial nucleoids Open
Acylation modifications, such as the succinylation of lysine, are post-translational modifications and a powerful means of regulating protein activity. Some acylations occur nonenzymatically, driven by an increase in the concentration of a…
View article: Mitochondrial HSP70 Chaperone System—The Influence of Post-Translational Modifications and Involvement in Human Diseases
Mitochondrial HSP70 Chaperone System—The Influence of Post-Translational Modifications and Involvement in Human Diseases Open
Since their discovery, heat shock proteins (HSPs) have been identified in all domains of life, which demonstrates their importance and conserved functional role in maintaining protein homeostasis. Mitochondria possess several members of th…
View article: Mitochondrial Kinases and the Role of Mitochondrial Protein Phosphorylation in Health and Disease
Mitochondrial Kinases and the Role of Mitochondrial Protein Phosphorylation in Health and Disease Open
The major role of mitochondria is to provide cells with energy, but no less important are their roles in responding to various stress factors and the metabolic changes and pathological processes that might occur inside and outside the cell…
View article: Mitochondrial HMG-Box Containing Proteins: From Biochemical Properties to the Roles in Human Diseases
Mitochondrial HMG-Box Containing Proteins: From Biochemical Properties to the Roles in Human Diseases Open
Mitochondrial DNA (mtDNA) molecules are packaged into compact nucleo-protein structures called mitochondrial nucleoids (mt-nucleoids). Their compaction is mediated in part by high-mobility group (HMG)-box containing proteins (mtHMG protein…
View article: The role of Lon-mediated proteolysis in the dynamics of mitochondrial nucleic acid-protein complexes
The role of Lon-mediated proteolysis in the dynamics of mitochondrial nucleic acid-protein complexes Open
Mitochondrial nucleoids consist of several different groups of proteins, many of which are involved in essential cellular processes such as the replication, repair and transcription of the mitochondrial genome. The eukaryotic, ATP-dependen…
View article: The N-terminal domain plays a crucial role in the structure of a full-length human mitochondrial Lon protease
The N-terminal domain plays a crucial role in the structure of a full-length human mitochondrial Lon protease Open
Lon is an essential, multitasking AAA + protease regulating many cellular processes in species across all kingdoms of life. Altered expression levels of the human mitochondrial Lon protease ( h Lon) are linked to serious diseases including…
View article: The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome
The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome Open
To study the mechanisms involved in the maintenance of a linear mitochondrial genome we investigated the biochemical properties of the recombination protein Mgm101 from Candida parapsilosis. We show that CpMgm101 complements defects associ…