Volker Westphal
YOU?
Author Swipe
View article: MINFLUX fluorescence nanoscopy in biological tissue
MINFLUX fluorescence nanoscopy in biological tissue Open
Optical imaging access to nanometer-level protein distributions in intact tissue is a highly sought-after goal, as it would provide visualization in physiologically relevant contexts. Under the unfavorable signal-to-background conditions o…
View article: MINFLUX fluorescence nanoscopy in biological tissue
MINFLUX fluorescence nanoscopy in biological tissue Open
Optical imaging access to nanometer-level protein distributions in intact tissue is a highly sought-after goal, as it would provide visualization in physiologically relevant contexts. Under the unfavorable signal-to-background conditions o…
View article: Multicolour nanoscopy of fixed and living cells with a single STED beam and hyperspectral detection
Multicolour nanoscopy of fixed and living cells with a single STED beam and hyperspectral detection Open
The extension of fluorescence nanoscopy to larger numbers of molecular species concurrently visualized by distinct markers is of great importance for advanced biological applications. To date, up to four markers had been distinguished in S…
View article: A protocol for registration and correction of multicolour STED superresolution images
A protocol for registration and correction of multicolour STED superresolution images Open
Summary Multicolour fluorescence imaging by STimulated Emission Depletion (STED) superresolution microscopy with doughnut‐shaped STED laser beams based on different wavelengths for each colour channel requires precise image registration. T…
View article: Ground State Depletion Nanoscopy Resolves Semiconductor Nanowire Barcode Segments at Room Temperature
Ground State Depletion Nanoscopy Resolves Semiconductor Nanowire Barcode Segments at Room Temperature Open
Nanowires hold great promise as tools for probing and interacting with various molecular and biological systems. Their unique geometrical properties (typically <100 nm in diameter and a few micrometers in length) enable minimally invasive …
View article: Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent
Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent Open
Significance In stimulated emission depletion (STED) fluorescence microscopy, the diffraction resolution barrier is overcome by applying a doughnut-shaped light beam that transiently switches fluorescence off, confining molecular emission …
View article: Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes
Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes Open
Superresolution imaging in sharper focus An optical microscope cannot distinguish objects separated by less than half the wavelength of light. Superresolution techniques have broken this “diffraction limit” and provided exciting new insigh…