Stefan Barth
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View article: The Therapeutic Potential of Photoimmunotherapy as a Safe, Effective and Non‐Toxic Treatment Option for Superficial Triple Negative Breast Cancer
The Therapeutic Potential of Photoimmunotherapy as a Safe, Effective and Non‐Toxic Treatment Option for Superficial Triple Negative Breast Cancer Open
Triple‐negative breast cancer (TNBC) is the most aggressive breast cancer subtype, lacking estrogen (ER), progesterone (PR), and human epidermal growth factor receptor (HER‐2) expression. It disproportionately affects women of African desc…
View article: SNAP‐Tag–Based Recombinant Photoimmunotherapeutic Agents for the Selective Detection and Killing of Light‐Accessible Melanotransferrin‐Expressing Melanoma and Triple‐Negative Breast Cancer
SNAP‐Tag–Based Recombinant Photoimmunotherapeutic Agents for the Selective Detection and Killing of Light‐Accessible Melanotransferrin‐Expressing Melanoma and Triple‐Negative Breast Cancer Open
Background Melanoma and triple negative breast cancer (TNBC) represent the most aggressive skin and breast cancer subtypes and are associated with poor diagnostic and limited therapeutic options leading to poor prognosis. Melanotransferrin…
View article: Tumor suppressor protein-inspired peptide for siRNA delivery and synergistic cancer therapy
Tumor suppressor protein-inspired peptide for siRNA delivery and synergistic cancer therapy Open
Small interfering RNA (siRNA) has shown promising therapeutic prospects in many major diseases. However, two main reasons limit the application of siRNA: poor endocytosis efficiency and weak endosomal escape ability. Therefore, the develop…
View article: Anti-CD64(scFv)-SNAP-Auristatin F: An in vitro proof of concept study for a recombinant antibody conjugate targeting CD64+ acute monocytic leukemia
Anti-CD64(scFv)-SNAP-Auristatin F: An in vitro proof of concept study for a recombinant antibody conjugate targeting CD64+ acute monocytic leukemia Open
Acute monocytic leukemia (AML-M5) is a rapidly progressing hematological malignancy characterized by limited therapeutic options and poor survival outcomes. Conventional therapies, such as intensive chemotherapy offer limited efficacy due …
View article: Hypercoagulability identified in dogs with chronic enteropathy using a point‐of‐care viscoelastic assay
Hypercoagulability identified in dogs with chronic enteropathy using a point‐of‐care viscoelastic assay Open
Objectives Thromboelastography (TEG) using the TEG 6s, a point‐of‐care viscoelastic assay, was prospectively evaluated in dogs with chronic enteropathy (CE). Additionally, the study determined whether disease activity, assessed using the C…
View article: AAV Capsid Modification and Its Influence on Viral Protein Stoichiometry and Packaging Fitness: Current Understandings and Future Direction
AAV Capsid Modification and Its Influence on Viral Protein Stoichiometry and Packaging Fitness: Current Understandings and Future Direction Open
The field of gene therapy has witnessed significant advancements in the utilization of Adeno-associated virus (AAV) owing to its inherent biological advantages. Targeted AAV vectors are generated through genetic or chemical modification of…
View article: Serpin B9‐Insensitive Granzyme B Mutant Delivered by Engineered Capsid AAV Vectors Demonstrates Selective Killing of EGFR‐Positive Cancer Cells
Serpin B9‐Insensitive Granzyme B Mutant Delivered by Engineered Capsid AAV Vectors Demonstrates Selective Killing of EGFR‐Positive Cancer Cells Open
Cellular immunotherapy strategies that harness the cytotoxic properties of T and NK cells by releasing granzyme B have emerged as a crucial approach in cancer therapy for both hematological and solid tumors. However, the effective release …
View article: Carrier-Free Peptide–Daunorubicin–Small Interfering RNA Nanoassembly for Targeted Therapy of Acute Myeloid Leukemia
Carrier-Free Peptide–Daunorubicin–Small Interfering RNA Nanoassembly for Targeted Therapy of Acute Myeloid Leukemia Open
Acute myeloid leukemia (AML) continues to represent a substantial unmet therapeutic need in clinical practice. In recent years, peptide–drug conjugates and small interfering RNA (siRNA) drugs have gained considerable attention due to their…
View article: EpCAM-targeting cancer immunotherapies: Evidence from clinical studies and the way forward
EpCAM-targeting cancer immunotherapies: Evidence from clinical studies and the way forward Open
Overexpressed cell-surface receptors play a crucial role as biomarkers in immunodiagnostics and immunotherapy. Epithelial cell adhesion molecule (EpCAM) is a 40 kDa cell-membrane glycoprotein associated with cell differentiation, survival,…
View article: Revisiting immunotherapeutic strategies for the management of atopic dermatitis
Revisiting immunotherapeutic strategies for the management of atopic dermatitis Open
Atopic dermatitis (AD) represents the most common inflammatory skin disease with a highly intricated immune fingerprint. Until recently, AD management mostly relied on topical corticosteroids, calcineurin inhibitors, and systemic immunosup…
View article: The Capacity of Drug-Metabolising Enzymes in Modulating the Therapeutic Efficacy of Drugs to Treat Rhabdomyosarcoma
The Capacity of Drug-Metabolising Enzymes in Modulating the Therapeutic Efficacy of Drugs to Treat Rhabdomyosarcoma Open
Rhabdomyosarcoma (RMS) is a rare soft tissue sarcoma (STS) that predominantly affects children and teenagers. It is the most common STS in children (40%) and accounts for 5–8% of total childhood malignancies. Apart from surgery and radioth…
View article: Machine learning and bioinformatic analyses link the cell surface receptor transcript levels to the drug response of breast cancer cells and drug off-target effects
Machine learning and bioinformatic analyses link the cell surface receptor transcript levels to the drug response of breast cancer cells and drug off-target effects Open
Breast cancer responds variably to anticancer therapies, often leading to significant off-target effects. This study proposes that the variability in tumour responses and drug-induced adverse events is linked to the transcriptional profile…
View article: Epidemiology of 7375 children and adolescents hospitalized with COVID-19 in Germany, reported via a prospective, nationwide surveillance study in 2020–2022
Epidemiology of 7375 children and adolescents hospitalized with COVID-19 in Germany, reported via a prospective, nationwide surveillance study in 2020–2022 Open
By means of a nationwide, prospective, multicenter, observational cohort registry collecting data on 7375 patients with laboratory-confirmed SARS-CoV-2 admitted to children's hospitals in Germany, March 2020–November 2022, our study assess…
View article: AAV Immunotoxicity: Implications in Anti-HBV Gene Therapy
AAV Immunotoxicity: Implications in Anti-HBV Gene Therapy Open
Hepatitis B virus (HBV) has afflicted humankind for decades and there is still no treatment that can clear the infection. The development of recombinant adeno-associated virus (rAAV)-based gene therapy for HBV infection has become importan…
View article: CSPG4 as a target for the specific killing of triple-negative breast cancer cells by a recombinant SNAP-tag-based antibody-auristatin F drug conjugate
CSPG4 as a target for the specific killing of triple-negative breast cancer cells by a recombinant SNAP-tag-based antibody-auristatin F drug conjugate Open
Purpose Triple-negative breast cancer (TNBC) is phenotypic of breast tumors lacking expression of the estrogen receptor (ER), the progesterone receptor (PgR), and the human epidermal growth factor receptor 2 (HER2). The paucity of well-def…
View article: Technological advances in the use of viral and non-viral vectors for delivering genetic and non-genetic cargos for cancer therapy
Technological advances in the use of viral and non-viral vectors for delivering genetic and non-genetic cargos for cancer therapy Open
The burden of cancer is increasing globally. Several challenges facing its mainstream treatment approaches have formed the basis for the development of targeted delivery systems to carry and distribute anti-cancer payloads to their defined…
View article: Data from EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein
Data from EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein Open
In normal epithelia, the epithelial cell adhesion molecule (EpCAM) expression is relatively low and only present at the basolateral cell surface. In contrast, EpCAM is aberrantly overexpressed in various human carcinomas. Therefore, EpCAM …
View article: Data from A Novel Recombinant Anti-CD22 Immunokinase Delivers Proapoptotic Activity of Death-Associated Protein Kinase (DAPK) and Mediates Cytotoxicity in Neoplastic B Cells
Data from A Novel Recombinant Anti-CD22 Immunokinase Delivers Proapoptotic Activity of Death-Associated Protein Kinase (DAPK) and Mediates Cytotoxicity in Neoplastic B Cells Open
The serine/threonine death-associated protein kinases (DAPK) provide pro-death signals in response to (oncogenic) cellular stresses. Lost DAPK expression due to (epi)genetic silencing is found in a broad spectrum of cancers. Within B-cell …
View article: Lilienthal N et al., Immunokinase-based B-lymphotoxic delivery of activated DAPK1; Supplements from A Novel Recombinant Anti-CD22 Immunokinase Delivers Proapoptotic Activity of Death-Associated Protein Kinase (DAPK) and Mediates Cytotoxicity in Neoplastic B Cells
Lilienthal N et al., Immunokinase-based B-lymphotoxic delivery of activated DAPK1; Supplements from A Novel Recombinant Anti-CD22 Immunokinase Delivers Proapoptotic Activity of Death-Associated Protein Kinase (DAPK) and Mediates Cytotoxicity in Neoplastic B Cells Open
Table S1: Expression of DAPK1 (a) and surface CD22 (b) in various cell lines; Table S2: Characteristics of CLL patients from whom samples were analyzed including for DAPK1/2 expression and responses to DK1KD-SGIII;Figure S1: Analysis of DA…
View article: Data from EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein
Data from EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein Open
In normal epithelia, the epithelial cell adhesion molecule (EpCAM) expression is relatively low and only present at the basolateral cell surface. In contrast, EpCAM is aberrantly overexpressed in various human carcinomas. Therefore, EpCAM …
View article: Data Supplement from EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein
Data Supplement from EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein Open
Supplementary Figure 1. Cell-binding activity of anti-EpCAM(scFv)-MAP to further target cell lines.
View article: Data from A Novel Recombinant Anti-CD22 Immunokinase Delivers Proapoptotic Activity of Death-Associated Protein Kinase (DAPK) and Mediates Cytotoxicity in Neoplastic B Cells
Data from A Novel Recombinant Anti-CD22 Immunokinase Delivers Proapoptotic Activity of Death-Associated Protein Kinase (DAPK) and Mediates Cytotoxicity in Neoplastic B Cells Open
The serine/threonine death-associated protein kinases (DAPK) provide pro-death signals in response to (oncogenic) cellular stresses. Lost DAPK expression due to (epi)genetic silencing is found in a broad spectrum of cancers. Within B-cell …
View article: Lilienthal N et al., Immunokinase-based B-lymphotoxic delivery of activated DAPK1; Supplements from A Novel Recombinant Anti-CD22 Immunokinase Delivers Proapoptotic Activity of Death-Associated Protein Kinase (DAPK) and Mediates Cytotoxicity in Neoplastic B Cells
Lilienthal N et al., Immunokinase-based B-lymphotoxic delivery of activated DAPK1; Supplements from A Novel Recombinant Anti-CD22 Immunokinase Delivers Proapoptotic Activity of Death-Associated Protein Kinase (DAPK) and Mediates Cytotoxicity in Neoplastic B Cells Open
Table S1: Expression of DAPK1 (a) and surface CD22 (b) in various cell lines; Table S2: Characteristics of CLL patients from whom samples were analyzed including for DAPK1/2 expression and responses to DK1KD-SGIII;Figure S1: Analysis of DA…
View article: Data Supplement from EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein
Data Supplement from EpCAM-Selective Elimination of Carcinoma Cells by a Novel MAP-Based Cytolytic Fusion Protein Open
Supplementary Figure 1. Cell-binding activity of anti-EpCAM(scFv)-MAP to further target cell lines.
View article: Click Chemistry-Generated Auristatin F–Linker–Benzylguanine for a SNAP-Tag-Based Recombinant Antibody–Drug Conjugate Demonstrating Selective Cytotoxicity toward EGFR-Overexpressing Tumor Cells
Click Chemistry-Generated Auristatin F–Linker–Benzylguanine for a SNAP-Tag-Based Recombinant Antibody–Drug Conjugate Demonstrating Selective Cytotoxicity toward EGFR-Overexpressing Tumor Cells Open
Antibody-drug conjugates (ADCs) are bifunctional molecules combining the targeting potential of monoclonal antibodies with the cancer-killing ability of cytotoxic drugs. This simple yet intelligently designed system directly addresses the …
View article: Combination of probiotics, plant extracts and micronutrients shows positive effects on the integrity of the intestinal barrier
Combination of probiotics, plant extracts and micronutrients shows positive effects on the integrity of the intestinal barrier Open
Functional disorders of the gastrointestinal tract cause symptoms that can have a significant impact on the quality of life. Maintaining an intact intestinal barrier is essential for preventing and treating these conditions. Initial studie…
View article: A recombinant Der p 1-specific allergen-toxin demonstrates superior killing of allergen-reactive IgG+ hybridomas in comparison to its recombinant allergen-drug conjugate
A recombinant Der p 1-specific allergen-toxin demonstrates superior killing of allergen-reactive IgG+ hybridomas in comparison to its recombinant allergen-drug conjugate Open
Summary Introduction Current treatments for asthma help to alleviate clinical symptoms but do not cure the disease. In this study, we explored a novel therapeutic approach for the treatment of house dust mite allergen Der p 1induced asthma…
View article: 1369 CD64-directed scFv fusion protein exhibits cytotoxicity and is a tool for site-specific diagnosis of acute myeloid leukemia
1369 CD64-directed scFv fusion protein exhibits cytotoxicity and is a tool for site-specific diagnosis of acute myeloid leukemia Open
Background Cancer immunotherapy is a promising innovative and effective treatment for many forms of cancer. Among haematological malignancies, acute myeloid leukemia (AML) remains an unmet medical need as it is mainly treated with chemothe…
View article: A Machine Learning and Bioinformatic Analysis Reveals an Associated between Cell Surface Receptor Transcript Levels with Drug Response of Breast Cancer Cells and the Drug Off-Target Effects
A Machine Learning and Bioinformatic Analysis Reveals an Associated between Cell Surface Receptor Transcript Levels with Drug Response of Breast Cancer Cells and the Drug Off-Target Effects Open
Breast cancer is characterised by varied responses to different anticancer therapies, which may provoke several different off-target effects. We hypothesise that for drugs that target cell surface receptors (CSRs), the different responses …