Walter Keller
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View article: Major Cat Allergen Fel d 4: Structure and Identification of a Cross‐Reactive <scp>IgE</scp> ‐Epitope‐Containing Area
Major Cat Allergen Fel d 4: Structure and Identification of a Cross‐Reactive <span>IgE</span> ‐Epitope‐Containing Area Open
Background Allergic sensitization to cats and other furry animals is a major cause of asthma and allergic rhinitis in more than 200 million people worldwide. According to the frequency of IgE recognition, allergen‐specific IgE levels, and …
View article: Der p 23, A Frequent IgE Sensitizer in Humans, Induces Airway Inflammation in Mice
Der p 23, A Frequent IgE Sensitizer in Humans, Induces Airway Inflammation in Mice Open
Der p 23 induces a high-frequency sensitization in allergic individuals. However, its allergenic activity and clinical impact are scarce. We aimed to evaluate the ability of rDer p 23 to induce allergic inflammation in a mouse model and to…
View article: Immunoinformatics Design and Identification of B-Cell Epitopes from Vespa affinis PLA1 Allergen
Immunoinformatics Design and Identification of B-Cell Epitopes from Vespa affinis PLA1 Allergen Open
Phospholipase A1 (Ves a 1), a major toxin from Vespa affinis venom, poses significant risks to allergic individuals. Nevertheless, the epitope determinants of Ves a 1 have not been characterized. Thus, identifying its linear B-cell epitope…
View article: Recombinant Hypoallergenic Cat Allergy Vaccines
Recombinant Hypoallergenic Cat Allergy Vaccines Open
Background Molecular forms of allergen‐specific immunotherapy (AIT) for cat allergy are needed. Fel d 1, Fel d 4, and Fel d 7 are the most important cat allergens. Methods IgE epitopes of Fel d 4 and Fel d 7 were mapped by blocking allergi…
View article: The cytoskeletal protein profilin is an important allergen in saltwort (Salsola kali)
The cytoskeletal protein profilin is an important allergen in saltwort (Salsola kali) Open
Pollen from Salsola kali , i.e., saltwort, Russian thistle, is a major allergen source in the coastal regions of southern Europe, in Turkey, Central Asia, and Iran. S. kali- allergic patients mainly suffer from hay-fever (i.e., rhinitis an…
View article: The molecular architecture of <i>Lactobacillus</i> S-layer: Assembly and attachment to teichoic acids
The molecular architecture of <i>Lactobacillus</i> S-layer: Assembly and attachment to teichoic acids Open
S-layers are crystalline arrays found on bacterial and archaeal cells. Lactobacillus is a diverse family of bacteria known especially for potential gut health benefits. This study focuses on the S-layer proteins from Lactobacillus acidophi…
View article: Vaccine Based on Recombinant Fusion Protein Combining Hepatitis B Virus PreS with SARS-CoV-2 Wild-Type- and Omicron-Derived Receptor Binding Domain Strongly Induces Omicron-Neutralizing Antibodies in a Murine Model
Vaccine Based on Recombinant Fusion Protein Combining Hepatitis B Virus PreS with SARS-CoV-2 Wild-Type- and Omicron-Derived Receptor Binding Domain Strongly Induces Omicron-Neutralizing Antibodies in a Murine Model Open
Background: COVID-19, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a recurrent endemic disease affecting the whole world. Since November 2021, Omicron and its subvariants have dominated in the spre…
View article: Effect of TraN key residues involved in DNA binding on pIP501 transfer rates in Enterococcus faecalis
Effect of TraN key residues involved in DNA binding on pIP501 transfer rates in Enterococcus faecalis Open
Conjugation is a major mechanism that facilitates the exchange of antibiotic resistance genes among bacteria. The broad-host-range Inc18 plasmid pIP501 harbors 15 genes that encode for a type IV secretion system (T4SS). It is a membrane-sp…
View article: Vaccine Based on Recombinant Fusion Protein Combining HBV PreS with SARS-CoV-2 Wild-Type- and Omicron-Derived RBD Strongly Induces Omicron-Neutralizing Antibodies
Vaccine Based on Recombinant Fusion Protein Combining HBV PreS with SARS-CoV-2 Wild-Type- and Omicron-Derived RBD Strongly Induces Omicron-Neutralizing Antibodies Open
Background: COVID-19 caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a recurrent endemic disease affecting the whole world. Since November 2021, Omicron and its subvariants are dominating. In order to …
View article: The molecular architecture of Lactobacillus S-Layer: Assembly and attachment to teichoic acids
The molecular architecture of Lactobacillus S-Layer: Assembly and attachment to teichoic acids Open
S-layers are crystalline arrays found on bacterial and archaeal cells. Lactobacillus is a diverse family of bacteria known especially for potential gut health benefits. This study focuses on the S-layer proteins from Lactobacillus acidophi…
View article: Allergenic Activity of Individual Cat Allergen Molecules
Allergenic Activity of Individual Cat Allergen Molecules Open
More than 10% of the world’s population suffers from an immunoglobulin E (IgE)-mediated allergy to cats which is accompanied mainly by respiratory symptoms such as rhinitis and asthma. Several cat allergen molecules have been identified, b…
View article: Recombinant PreS‐fusion protein vaccine for birch pollen and apple allergy
Recombinant PreS‐fusion protein vaccine for birch pollen and apple allergy Open
Background IgE cross‐sensitization to major birch pollen allergen Bet v 1 and pathogenesis‐related (PR10) plant food allergens is responsible for the pollen‐food allergy syndrome. Methods We designed a recombinant protein, AB‐PreS, consist…
View article: Recombinant PreS-fusion protein vaccine for birch pollen and apple allergy
Recombinant PreS-fusion protein vaccine for birch pollen and apple allergy Open
Background: Respiratory birch pollen allergy and associated oral allergy syndrome affect more than 150 million people. IgE cross-sensitization to major birch pollen allergen Bet v 1 and pathogenesis-related (PR10) plant food allergens is r…
View article: Art v 1 IgE epitopes of patients and humanized mice are conformational
Art v 1 IgE epitopes of patients and humanized mice are conformational Open
Identification of the new major conformational IgE binding site on Art v 1, which can be blocked with IgG raised against non-IgE reactive Art v 1 peptides, is an important basis for the development of a hypoallergenic peptide vaccine for m…
View article: Identification of Epitopes on Rhinovirus 89 Capsid Proteins Capable of Inducing Neutralizing Antibodies
Identification of Epitopes on Rhinovirus 89 Capsid Proteins Capable of Inducing Neutralizing Antibodies Open
Rhinoviruses (RVs) are major causes of the common cold, but they can also trigger exacerbations of asthma. More than 160 different RV strains exist and can be classified into three genetic species (RV-A, RV-B and RV-C) which bind to differ…
View article: Small Things Matter: The 11.6-kDa TraB Protein is Crucial for Antibiotic Resistance Transfer Among Enterococci
Small Things Matter: The 11.6-kDa TraB Protein is Crucial for Antibiotic Resistance Transfer Among Enterococci Open
Conjugative transfer is the most important means for spreading antibiotic resistance genes. It is used by Gram-positive and Gram-negative bacteria, and archaea as well. Conjugative transfer is mediated by molecular membrane-spanning nanoma…
View article: Vaccine based on folded receptor binding domain‐PreS fusion protein with potential to induce sterilizing immunity to SARS‐CoV‐2 variants
Vaccine based on folded receptor binding domain‐PreS fusion protein with potential to induce sterilizing immunity to SARS‐CoV‐2 variants Open
Background Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is responsible for the ongoing global COVID‐19 pandemic. One possibility to control the pandemic is to induce sterilizing immunity through the induction and maintenanc…
View article: Neutralization of SARS‐CoV‐2 requires antibodies against conformational receptor‐binding domain epitopes
Neutralization of SARS‐CoV‐2 requires antibodies against conformational receptor‐binding domain epitopes Open
Background The determinants of successful humoral immune response to the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) are of critical importance for the design of effective vaccines and the evaluation of the degree of prote…
View article: IgE Epitopes of the House Dust Mite Allergen Der p 7 Are Mainly Discontinuous and Conformational
IgE Epitopes of the House Dust Mite Allergen Der p 7 Are Mainly Discontinuous and Conformational Open
Background Several studies indicate that Der p 7 is an important and clinically relevant allergen of Dermatophagoides pteronyssinus which should be included in vaccines for treatment of house dust mite (HDM) allergy. Aim of this study was …
View article: Structural and allergenic properties of the Fatty Acid Binding Protein from shrimp Litopenaeus vannamei
Structural and allergenic properties of the Fatty Acid Binding Protein from shrimp Litopenaeus vannamei Open
Background: The shrimp Litopaneus vanammei is an important source of food allergens but its allergenic repertoire is poorly characterized. Cross reactivity between crustacean and mites has been characterized, with tropomyosin, the most rel…
View article: Front Cover: Metal Ion Promiscuity and Structure of 2,3‐Dihydroxybenzoic Acid Decarboxylase of <i>Aspergillus oryzae</i> (ChemBioChem 4/2021)
Front Cover: Metal Ion Promiscuity and Structure of 2,3‐Dihydroxybenzoic Acid Decarboxylase of <i>Aspergillus oryzae</i> (ChemBioChem 4/2021) Open
Benzoic acid decarboxylases have been explored as efficient biocatalysts for the (de)carboxylation of electron-rich (hetero)aromatics. As members of the amidohydrolase superfamily, these enzymes share considerable structural and mechanisti…
View article: Expression in <i>Escherichia coli</i> and Purification of Folded rDer p 20, the Arginine Kinase From <i>Dermatophagoides pteronyssinus</i>: A Possible Biomarker for Allergic Asthma
Expression in <i>Escherichia coli</i> and Purification of Folded rDer p 20, the Arginine Kinase From <i>Dermatophagoides pteronyssinus</i>: A Possible Biomarker for Allergic Asthma Open
Arginine kinase (AK) was first identified as an allergen in the Indian-meal moth and subsequently shown to occur as allergen in various invertebrates and shellfish. The cDNA coding for AK from the house dust mite (HDM) species Dermatophago…
View article: Metal Ion Promiscuity and Structure of 2,3‐Dihydroxybenzoic Acid Decarboxylase of <i>Aspergillus oryzae</i>
Metal Ion Promiscuity and Structure of 2,3‐Dihydroxybenzoic Acid Decarboxylase of <i>Aspergillus oryzae</i> Open
Broad substrate tolerance and excellent regioselectivity, as well as independence from sensitive cofactors have established benzoic acid decarboxylases from microbial sources as efficient biocatalysts. Robustness under process conditions m…
View article: Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1
Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1 Open
Birch pollen allergy is among the most prevalent pollen allergies in Northern and Central Europe. This IgE-mediated disease can be treated with allergen immunotherapy (AIT), which typically gives rise to IgG antibodies inducing tolerance. …
View article: Regioselective carboxylation by prenylated flavin and Mn-dependent decarboxylases
Regioselective carboxylation by prenylated flavin and Mn-dependent decarboxylases Open
Carboxylation reactions received considerable attention in view of the use of CO 2 as abundant C 1 -building block for sustainable chemical production.However, to date only a few examples of CO 2 fixation reactions have been realized on in…
View article: Profilins and the quest for structure-based IgE-epitope mapping
Profilins and the quest for structure-based IgE-epitope mapping Open
Background: Panallergens frequently cause respiratory allergy and oral allergy syndrome.Profilins are important panallergens because of their highly conserved structure and ubiquitous presence in allergy sources.Therefore, determination of…