Robert W. Sauerwein
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View article: Multi-omics analysis reveals distinct gene regulatory mechanisms between primary and organoid-derived human hepatocytes
Multi-omics analysis reveals distinct gene regulatory mechanisms between primary and organoid-derived human hepatocytes Open
Hepatic organoid cultures are a powerful model to study liver development and diseases in vitro. However, hepatocyte-like cells differentiated from these organoids remain immature compared to primary human hepatocytes (PHHs), which are the…
View article: Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross-stage recognition of glutamate-rich repeats
Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross-stage recognition of glutamate-rich repeats Open
Circulating sexual stages of Plasmodium falciparum (Pf ) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies can efficiently block parasite transm…
View article: Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats
Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats Open
Circulating sexual stages of Plasmodium falciparum (Pf) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies (Abs) can efficiently block parasite t…
View article: Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross-stage recognition of glutamate-rich repeats
Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross-stage recognition of glutamate-rich repeats Open
Circulating sexual stages of Plasmodium falciparum (Pf ) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies can efficiently block parasite transm…
View article: Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats
Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats Open
Circulating sexual stages of Plasmodium falciparum (Pf) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies (Abs) can efficiently block parasite t…
View article: Reviewer #1 (Public Review): Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats
Reviewer #1 (Public Review): Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats Open
Circulating sexual stages of Plasmodium falciparum (Pf) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies (Abs) can efficiently block parasite t…
View article: Reviewer #3 (Public Review): Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats
Reviewer #3 (Public Review): Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats Open
Circulating sexual stages of Plasmodium falciparum (Pf) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies (Abs) can efficiently block parasite t…
View article: Modulation of host signalling pathways reveal a major role for Wnt signalling in the maturation of <i>Plasmodium falciparum</i> liver schizonts
Modulation of host signalling pathways reveal a major role for Wnt signalling in the maturation of <i>Plasmodium falciparum</i> liver schizonts Open
After infection of the human host, the initial stage of the Plasmodium falciparum (Pf) lifecycle takes place in the liver. However, understanding of the host-parasite interaction has been limited by the rapid loss of functionality in cultu…
View article: Integrative omics analysis reveals gene regulatory mechanisms distinguishing organoid-derived hepatocytes from primary human hepatocytes
Integrative omics analysis reveals gene regulatory mechanisms distinguishing organoid-derived hepatocytes from primary human hepatocytes Open
Background and Aims Hepatic organoid cultures are considered a powerful model system to study liver development and diseases in vitro . However, hepatocyte-like cells differentiated from such organoids remain immature compared to primary h…
View article: Target-agnostic identification of human antibodies to <i>Plasmodium falciparum</i> sexual forms reveals cross stage recognition of glutamate-rich repeats
Target-agnostic identification of human antibodies to <i>Plasmodium falciparum</i> sexual forms reveals cross stage recognition of glutamate-rich repeats Open
Circulating sexual stages of Plasmodium falciparum (Pf) can be transmitted from humans to mosquitoes, thereby furthering the spread of malaria in the population. It is well established that antibodies (Abs) can efficiently block parasite t…
View article: Sporozoite immunization: innovative translational science to support the fight against malaria
Sporozoite immunization: innovative translational science to support the fight against malaria Open
Introduction Malaria, a devastating febrile illness caused by protozoan parasites, sickened 247,000,000 people in 2021 and killed 619,000, mostly children and pregnant women in sub-Saharan Africa. A highly effective vaccine is urgently nee…
View article: Development of Plasmodium falciparum liver-stages in hepatocytes derived from human fetal liver organoid cultures
Development of Plasmodium falciparum liver-stages in hepatocytes derived from human fetal liver organoid cultures Open
Plasmodium falciparum ( Pf ) parasite development in liver represents the initial step of the life-cycle in the human host after a Pf- infected mosquito bite. While an attractive stage for life-cycle interruption, understanding of parasite…
View article: Baseline TGF-β correlates with protection after immunization with Plasmodium falciparum sporozoites in the controlled human malaria infection model
Baseline TGF-β correlates with protection after immunization with Plasmodium falciparum sporozoites in the controlled human malaria infection model Open
Baseline TGF-β concentrations predict the efficiency of acquisition of sterile immunity following sporozoite immunization and may represent a steady-state regulatory mechanism to keep in check immune systems with a low threshold for activa…
View article: Whole sporozoite immunization with Plasmodium falciparum strain NF135 in a randomized trial
Whole sporozoite immunization with Plasmodium falciparum strain NF135 in a randomized trial Open
Background Whole sporozoite immunization under chemoprophylaxis (CPS regime) induces long-lasting sterile homologous protection in the controlled human malaria infection model using Plasmodium falciparum strain NF54. The relative proficien…
View article: Plasmodium falciparum malaria exposure and carriage associate with reduced γδ T-cells and NK cell responses to infected red blood cells in vitro
Plasmodium falciparum malaria exposure and carriage associate with reduced γδ T-cells and NK cell responses to infected red blood cells in vitro Open
Background Innate immune cells including γδ T-cells and NK cells are directly activated by Plasmodium falciparum parasites and contribute to the control of parasitaemia. The aim of this study was to determine whether a history of parasite …
View article: Assessment of the drugability of initial malaria infection through miniaturized sporozoite assays and high-throughput screening
Assessment of the drugability of initial malaria infection through miniaturized sporozoite assays and high-throughput screening Open
The sporozoite stages of malaria parasites are the primary cause of infection of the vertebrate host and are targeted by (experimental) vaccines. Yet, little is known about their susceptibility to chemical intervention. Phenotypic high-thr…
View article: Highly potent, naturally acquired human monoclonal antibodies against Pfs48/45 block Plasmodium falciparum transmission to mosquitoes
Highly potent, naturally acquired human monoclonal antibodies against Pfs48/45 block Plasmodium falciparum transmission to mosquitoes Open
Malaria transmission-blocking vaccines (TBVs) aim to induce antibodies that interrupt malaria parasite development in the mosquito, thereby blocking onward transmission, and provide a much-needed tool for malaria control and elimination. T…
View article: Plasmodium falciparum Malaria Carriage Associates with Reduced γδ T-Cell and NK Cell Responses to Infected Red Blood Cells In Vitro
Plasmodium falciparum Malaria Carriage Associates with Reduced γδ T-Cell and NK Cell Responses to Infected Red Blood Cells In Vitro Open
Background: Innate immune cells including γδ T-cells and NK cells are directly activated by Plasmodium falciparum parasites and contribute to the control of parasitaemia. The aim of this study was to determine whether parasite carriage aff…
View article: Hijacking the human complement inhibitor C4b-binding protein by the sporozoite stage of the Plasmodium falciparum parasite
Hijacking the human complement inhibitor C4b-binding protein by the sporozoite stage of the Plasmodium falciparum parasite Open
The complement system is considered the first line of defense against pathogens. Hijacking complement regulators from blood is a common evasion tactic of pathogens to inhibit complement activation on their surfaces. Here, we report hijacki…
View article: Functional changes in hemostasis during asexual and sexual parasitemia in a controlled human malaria infection
Functional changes in hemostasis during asexual and sexual parasitemia in a controlled human malaria infection Open
Decreased platelet count is an early phenomenon in asexual Plasmodium falciparum parasitemia, but its association with acute or long-term functional changes in platelets and coagulation is unknown. Moreover, the impact of gametocytemia on …
View article: 40 Years of Pfs48/45 Research as a Transmission-Blocking Vaccine Target of Plasmodium falciparum Malaria
40 Years of Pfs48/45 Research as a Transmission-Blocking Vaccine Target of Plasmodium falciparum Malaria Open
In the early 1980s, Richard Carter was among the first researchers to identify the sexual stage-specific Pfs48/45 protein, leading to the identification of target epitopes. Carter predicted its tertiary conformation while involved in a num…
View article: Preclinical characterization and target validation of the antimalarial pantothenamide MMV693183
Preclinical characterization and target validation of the antimalarial pantothenamide MMV693183 Open
Drug resistance and a dire lack of transmission-blocking antimalarials hamper malaria elimination. Here, we present the pantothenamide MMV693183 as a first-in-class acetyl-CoA synthetase (AcAS) inhibitor to enter preclinical development. O…