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View article: Characterization of a β-Galactosidase from Kosakonia oryzendophytica and Its Heterologous Expression in Bacillus subtilis for Galactooligosaccharides Production
Characterization of a β-Galactosidase from Kosakonia oryzendophytica and Its Heterologous Expression in Bacillus subtilis for Galactooligosaccharides Production Open
Galactooligosaccharides (GOS) typically consist of 2-8 D-galactose units linked together, terminating in a D-glucose unit. GOS are commonly used in dairy products, infant formulas, and functional foods. GOS offer beneficial properties for …
View article: Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose Open
d-Mannose is an attractive functional sugar that exhibits many physiological benefits on human health. The demand for low-calorie sugars and sweeteners in foods are increasingly available on the market. Some sugar isomerases, such as d-lyx…
View article: Identification of a Thermostable Levansucrase from Pseudomonas orientalis That Allows Unique Product Specificity at Different Temperatures
Identification of a Thermostable Levansucrase from Pseudomonas orientalis That Allows Unique Product Specificity at Different Temperatures Open
The biological production of levan by levansucrase (LS, EC 2.4.1.10) has aroused great interest in the past few years. Previously, we identified a thermostable levansucrase from Celerinatantimonas diazotrophica (Cedi-LS). A novel thermosta…
View article: Characterization of<scp>l</scp>-fucose isomerase from<i>Paenibacillus rhizosphaerae</i>to produce<scp>l</scp>-fuculose from hydrolyzed fucoidan and commercial fucose
Characterization of<span>l</span>-fucose isomerase from<i>Paenibacillus rhizosphaerae</i>to produce<span>l</span>-fuculose from hydrolyzed fucoidan and commercial fucose Open
Aims l-Fuculose is a valuable rare sugar that is used to treat a variety of ailments, including HIV, cancer, Hepatitis B, human lysosomal disease (fucosidosis), and cardio-protective medications. The enzymatic approach for the production o…
View article: Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol Open
Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot s…
View article: Crystal Structure of Levansucrase from the Gram-Negative Bacterium <i>Brenneria</i> Provides Insights into Its Product Size Specificity
Crystal Structure of Levansucrase from the Gram-Negative Bacterium <i>Brenneria</i> Provides Insights into Its Product Size Specificity Open
Microbial levansucrases (LSs, EC 2.4.1.10) have been widely studied for the synthesis of β-(2,6)-fructans (levan) from sucrose. LSs synthesize levan-type fructo-oligosaccharides, high-molecular-mass levan polymer or combinations of both. H…
View article: Enzymatic Preparation of Gentiooligosaccharides by a Thermophilic and Thermostable β-Glucosidase at a High Substrate Concentration
Enzymatic Preparation of Gentiooligosaccharides by a Thermophilic and Thermostable β-Glucosidase at a High Substrate Concentration Open
Gentiooligosaccharides (GnOS) are a kind of oligosaccharide formed by glucose with β-1-6 glycosidic bonds, which has become a new type of functional oligosaccharide for its unique refreshing bitter taste and valuable probiotic effects. How…
View article: Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes
Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes Open
d-allulose has a significant application value as a sugar substitute, not only as a food ingredient and dietary supplement, but also with various physiological functions, such as improving insulin resistance, anti-obesity, and regulating g…
View article: Computer-aided search for a cold-active cellobiose 2-epimerase
Computer-aided search for a cold-active cellobiose 2-epimerase Open
Cellobiose 2-epimerase (CE) is a promising industrial enzyme that can catalyze bioconversion of lactose to its high-value derivatives, namely epilactose and lactulose. A need exists in the dairy industry to catalyze lactose bioconversions …
View article: CLONING, PURIFICATION AND CHARACTERIZATION OF A NOVEL RECOMBINANT TREHALOSE SYNTHASE (TreS) FROM Acidiplasma sp. MBA-1
CLONING, PURIFICATION AND CHARACTERIZATION OF A NOVEL RECOMBINANT TREHALOSE SYNTHASE (TreS) FROM Acidiplasma sp. MBA-1 Open
Trehalose, a nonreducing disaccharide, can be commercially produced using maltose from microorganisms. An intramolecular transglycosylase enzyme called trehalose synthase (TreS) can catalyze the conversion of maltose to trehalose in a sing…
View article: Construction of an enzymatic route using a food-grade recombinant Bacillus subtilis for the production and purification of epilactose from lactose
Construction of an enzymatic route using a food-grade recombinant Bacillus subtilis for the production and purification of epilactose from lactose Open
Lactose is a main by-product in the cheese industry. Many attempts have been made to convert the lactose to high value-added products, including epilactose. Epilactose is a valuable prebiotic and can be epimerized from lactose with cellobi…
View article: Synthesis of allitol from D-psicose using ribitol dehydrogenase and formate dehydrogenase
Synthesis of allitol from D-psicose using ribitol dehydrogenase and formate dehydrogenase Open
Purpose: To synthesize allitol from D-psicose by a combination of novel ribitol dehydrogenase (RDH) and formate dehydrogenase (FDH) under optimised production conditions.Methods: RDH and FDH genes were cloned and introduced into pET-22b(+)…