Explanipedia

Agaricus bisporus mannose-binding protein stimulates the innate immune cells Open

Heni Rachmawati, Agung Heru Kaarsono, Olivia Mayasari Tandrasasmita, Raymond R. Tjandrawinata, Wangsa T. Ismaya · 2024

Purpose: A lectin-like protein from the mushroom Agaricus bisporus has been shown to slightly increase the proliferation of RAW 264.7 cells. Following its identification as a mannose-binding lectin, henceforth called A. bisporus mannose-bi…

Direct relationship between dimeric form and activity in the acidic copper–zinc superoxide dismutase from lemon Open

Ratna Annisa Utami, Hiromi Yoshida, Lydia H. Kartadinata, Virgi A. Abdillah, Cut R. Faratilla , et al. · 2023

The copper–zinc superoxide dismutase (CuZnSOD) from lemon (SOD_CL) is active in an acidic environment and resists proteolytic degradation. The enzyme occurs as a dimer, which has an indirect effect on the enzyme activity as the monomer ret…

An elevated OmpA expression during the production of a recombinant protein in Escherichia coli Open

Frans Kurnia, Gestria Novirani, Fatiha Khairunnisa, Vincencius F. Meidianto, Wangsa T. Ismaya , et al. · 2023

Lectins from the Edible Mushroom Agaricus bisporus and Their Therapeutic Potentials Open

Wangsa T. Ismaya, Raymond R. Tjandrawinata, Heni Rachmawati · 2020

The mushroom Agaricus bisporus secretes biologically active compounds and proteins with benefits for human health. Most reported proteins from A. bisporus are tyrosinases and lectins. Lectins are of therapeutic or pharmaceutical interest. …

Preliminary insight into recognizing of mannose toward LSMT Protein: Molecular docking and DFT Study Open

Aswin Abbas, Mohammad Kemal Agusta, Hermawan Kresno Dipojono, Adhitya Gadaryus Saputro, Heni Rachmawati , et al. · 2018

The present study focuses on investigating the interaction between Mannose and LSMT using molecular docking and Density Functional Theory (DFT).A novel protein like-lectin Light Subunit Mushroom Tyrosinase (LSMT) was discovered inadvertent…

Bioconjugation of Captopril–Light Subunit of <i>Agaricus bisporus</i> Mushroom Tyrosinase: Characterization and Potential Use as a Drug Carrier for Oral Delivery Open

Diana Diana, Wangsa T. Ismaya, Vincencius F. Meidianto, Olivia Mayasari Tandrasasmita, Raymond R. Tjandrawinata , et al. · 2018

We show that a lectin like protein from the mushroom Agaricus bisporus (LSMT) is capable to permeate the epithelial monolayer barrier of the intestine ex vivo. The protein is not toxic or immunogenic upon prolonged administration and eleva…

The first crystal structure of manganese superoxide dismutase from the genus<i>Staphylococcus</i> Open

Debbie S. Retnoningrum, Hiromi Yoshida, Sekar Arumsari, S. Kamitori, Wangsa T. Ismaya · 2018

A recombinant Staphylococcus equorum manganese superoxide dismutase (MnSOD) with an Asp13Arg substitution displays activity over a wide range of pH, at high temperature and in the presence of chaotropic agents, and retains 50% of its activ…

Study of response of Swiss Webster mice to light subunit of mushroom tyrosinase Open

Wangsa T. Ismaya, Alida Efthyani, DS Retnoningrum, Xuelei Lai, Bauke W. Dijkstra , et al. · 2017

The light subunit of mushroom, Agaricus bisporus, tyrosinase (LSMT), has been identified as an extrinsic component of the enzyme. Its function is unknown, but it can cross an epithelial cell layer, which suggests that it can be absorbed by…

Food Origin Fibrinolytic Enzyme With Multiple Actions Open

Laurentia Stephani, Raymond R. Tjandrawinata, Diana Nur Afifah, Yanti Lim, Wangsa T. Ismaya , et al. · 2017

Many health related problems such as cardiovascular diseases are associated with the formation of excessive clot in the blood (thrombus). Approaches in cardiovascular disease treatment are preventing the formation or removing the thrombus.…

Obtención de un inhibidor de proteasas de leucocitos secretores humanos recombinante soluble y activo Open

Elly Munadziroh, Shindy Purnamasari, Ni Nyoman Tri Puspaningsih, Soetjipto Soetjipto, Muhammad Rubianto , et al. · 2017

Generation of a soluble and active recombinant human secretory leukocyte protease inhibitor Open

Elly Munadziroh, Shindy Purnamasari, Ni Nyoman Tri Puspaningsih, Soetjipto Soetjipto, Muhammad Rubianto , et al. · 2017

The light subunit of mushroom Agaricus bisporus tyrosinase: Its biological characteristics and implications Open

Wangsa T. Ismaya, Olivia Mayasari Tandrasasmita, Syaripah Sundari, Diana Diana, Xuelei Lai , et al. · 2017

A novel immune-tolerable and permeable lectin-like protein from mushroom Agaricus bisporus Open

Wangsa T. Ismaya, Yunita, Alida Efthyani, Xuelei Lai, Debbie S. Retnoningrum , et al. · 2016

Crystal structure of recombinant tyrosinase-binding protein MtaL at 1.35 Å resolution Open

Xuelei Lai, Montserrat Soler‐López, Wangsa T. Ismaya, Harry J. Wichers, Bauke W. Dijkstra · 2016

Mushroom tyrosinase-associated lectin-like protein (MtaL) binds to mature Agaricus bisporus tyrosinase in vivo , but the exact physiological function of MtaL is unknown. In this study, the crystal structure of recombinant MtaL is reported …

In Silico Study to Develop a Lectin-Like Protein from Mushroom Agaricus bisporus for Pharmaceutical Application Open

Wangsa T. Ismaya · 2016

A lectin-like protein of unknown function designated as LSMT was recently discovered in the edible mushroom Agaricus bisporus. The protein shares high structural similarity to HA-33 from Clostridium botulinum (HA33) and Ricin-B-like lectin…

Heterologous expression of -amylase from Saccharomycopsis fibuligera R64 and its Tyr401Trp mutant in Pichia pastoris Open

Riezki Amalia, Wangsa T. Ismaya, Fernita Puspasari, Khomaini Hasan, Toto Subroto , et al. · 2016

α-Amylase from Saccharomycopsis fibuligera R64 is a non-adsorbing raw-starch degrading enzyme, a unique characteristic. This character is difficult to explain in the absence of its three-dimensional structure. Here we discuss the expressio…

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