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View article: Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells
Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells Open
Primary human myeloid cells are promising candidates for immunotherapy, yet efficient and scalable technologies for genetic engineering and screening in these cells are limited. Here we present a virus-like particle (VLP)-based toolkit tha…
View article: Temporal photoproximity labeling of ligand-activated EGFR neighborhoods using MultiMap
Temporal photoproximity labeling of ligand-activated EGFR neighborhoods using MultiMap Open
Photoproximity labeling proteomics (PLP) methods have recently shown that cell surface receptors can form lateral interactome networks. Here, we present a paired set of PLP workflows that dynamically track neighborhood changes for oncogeni…
View article: Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity
Phenotypic screens for SIRPA expression reveal RAB21 as a general regulator of macrophage surface identity Open
Factors influencing the macrophage surfaceome define macrophage identity and behavior. Here, we use genome-wide phenotypic screens to identify genes affecting the accessibility and surface expression of macrophage signal regulatory protein…
View article: Cellular Glycocalyx Affects Nanoparticle Access to Cell Membranes and Uptake
Cellular Glycocalyx Affects Nanoparticle Access to Cell Membranes and Uptake Open
Understanding nanoparticle interactions with cells is fundamental to designing them for medical applications. Nanoparticles must interface with the cell surface to be bound and taken up. The glycocalyx is a carbohydrate layer coating the c…
View article: Packaged delivery of CRISPR–Cas9 ribonucleoproteins accelerates genome editing
Packaged delivery of CRISPR–Cas9 ribonucleoproteins accelerates genome editing Open
Effective genome editing requires a sufficient dose of CRISPR–Cas9 ribonucleoproteins (RNPs) to enter the target cell while minimizing immune responses, off-target editing, and cytotoxicity. Clinical use of Cas9 RNPs currently entails elec…
View article: Mechanism-guided engineering of a minimal biological particle for genome editing
Mechanism-guided engineering of a minimal biological particle for genome editing Open
The widespread application of genome editing to treat and cure disease requires the delivery of genome editors into the nucleus of target cells. Enveloped delivery vehicles (EDVs) are engineered virally derived particles capable of packagi…
View article: Spatiotemporal photocatalytic proximity labeling proteomics reveals ligand-activated extracellular and intracellular EGFR neighborhoods
Spatiotemporal photocatalytic proximity labeling proteomics reveals ligand-activated extracellular and intracellular EGFR neighborhoods Open
Photo-proximity labeling proteomics (PLP) methods have recently shown that the cell surface receptors can dynamically form lateral interactome networks. Here, we present a paired set of PLP workflows that simultaneously track neighborhood …
View article: Packaged delivery of CRISPR-Cas9 ribonucleoproteins accelerates genome editing
Packaged delivery of CRISPR-Cas9 ribonucleoproteins accelerates genome editing Open
Effective genome editing requires a sufficient dose of CRISPR-Cas9 ribonucleoproteins (RNPs) to enter the target cell while minimizing immune responses, off-target editing and cytotoxicity. Clinical use of Cas9 RNPs currently entails elect…
View article: Mechanism-guided engineering of a minimal biological particle for genome editing
Mechanism-guided engineering of a minimal biological particle for genome editing Open
The widespread application of genome editing to treat or even cure disease requires the delivery of genome editors into the nucleus of target cells. Enveloped Delivery Vehicles (EDVs) are engineered virally-derived particles capable of pac…
View article: In vivo human T cell engineering with enveloped delivery vehicles
In vivo human T cell engineering with enveloped delivery vehicles Open
Viruses and virally derived particles have the intrinsic capacity to deliver molecules to cells, but the difficulty of readily altering cell-type selectivity has hindered their use for therapeutic delivery. Here, we show that cell surface …
View article: Identifying cell receptors for the nanoparticle protein corona using genome screens
Identifying cell receptors for the nanoparticle protein corona using genome screens Open
This dataset is composed of the sequencing data used in the Nature Chemical Biology manuscript: https://www.nature.com/articles/s41589-022-01093-5 DOI: 10.1038/s41589-022-01093-5
View article: Surface-grafted polyethylene glycol conformation impacts the transport of PEG-functionalized liposomes through a tumour extracellular matrix model
Surface-grafted polyethylene glycol conformation impacts the transport of PEG-functionalized liposomes through a tumour extracellular matrix model Open
The effect of surface PEGylation on nanoparticle transport through an extracellular matrix (ECM) is an important determinant for tumor targeting success.