Explanipedia

Microphysiological Uremia Model Reveals Biophysical Potentiators of Vascular Dysfunction Open

Mitesh Rathod, Ryan Stack, Sarah Kubik, Stephanie Huang, Chloe P. Whitworth , et al. · 2025

Cardiovascular disease is a leading cause of mortality in individuals with chronic kidney disease (CKD), with hypertension being a major contributor to both kidney damage and increased cardiovascular risk. Despite the established link betw…

Colloidal-fibrillar composite gels demonstrate structural reinforcement, secondary fibrillar alignment, and improved vascular healing outcomes Open

Nina Moiseiwitsch, Sanika Pandit, Nicole Zwennes, Kimberly Nellenbach, Anastasia Sheridan , et al. · 2025

Many biological tissues contain colloids within a fibrillar structure. Here, we develop and characterize colloidal-fibrillar scaffolds through examination of the effects of relative colloid and fiber ratios within a fibrin-based model syst…

Dysfunctional mechanotransduction regulates the progression of PIK3CA-driven vascular malformations Open

Wen Yih Aw, Aanya Sawhney, Mitesh Rathod, Chloe P. Whitworth, Elizabeth L. Doherty , et al. · 2025

Somatic activating mutations in PIK3CA are common drivers of vascular and lymphatic malformations. Despite common biophysical signatures of tissues susceptible to lesion formation, including compliant extracellular matrix and low rates of …

Understanding the Lymphatic System: Tissue-on-Chip Modeling Open

William J. Polacheck, J. Brandon Dixon, Wen Yih Aw · 2025

The lymphatic vasculature plays critical roles in maintaining fluid homeostasis, transporting lipid, and facilitating immune surveillance. A growing body of work has identified lymphatic dysfunction as contributing to the severity of myria…

Microphysiological uremia model reveals biophysical potentiators of vascular dysfunction Open

Mitesh Rathod, Stephanie Huang, Wen Yih Aw, Elizabeth L. Doherty, Shane M. Meehan , et al. · 2024

Cardiovascular disease is a leading cause of mortality in individuals with chronic kidney disease. Hypertension, common among patients with chronic kidney disease, is a major contributor to both kidney damage and the heightened cardiovascu…

Donor‐Derived Engineered Microvessels for Cardiovascular Risk Stratification of Patients with Kidney Failure Open

Mitesh Rathod, Wen Yih Aw, Stephanie Huang, Jingming Lu, Elizabeth L. Doherty , et al. · 2024

Cardiovascular disease is the cause of death in ≈50% of hemodialysis patients. Accumulation of uremic solutes in systemic circulation is thought to be a key driver of the endothelial dysfunction that underlies elevated cardiovascular event…

Dysfunctional mechanotransduction regulates the progression of PIK3CA-driven vascular malformations Open

Wen Yih Aw, Aanya Sawhney, Mitesh Rathod, Chloe P. Whitworth, Elizabeth L. Doherty , et al. · 2024

Somatic activating mutations in PIK3CA are common drivers of vascular and lymphatic malformations. Despite common biophysical signatures of tissues susceptible to lesion formation, including compliant extracellular matrix and low rates of …

Human Cell‐Derived Matrix Composite Hydrogels with Diverse Composition for Use in Vasculature‐on‐chip Models Open

Elizabeth L. Doherty, Grace Krohn, Emily Warren, Alexandra Patton, Chloe P. Whitworth , et al. · 2024

Microphysiological and organ‐on‐chip platforms seek to address critical gaps in human disease models and drug development that underlie poor rates of clinical success for novel interventions. While the fabrication technology and model cell…

Donor‐Derived Engineered Microvessels for Cardiovascular Risk Stratification of Patients with Kidney Failure Open

Mitesh Rathod, Wen Yih Aw, Stephanie Huang, Jingming Lu, Elizabeth L. Doherty , et al. · 2024

Cardiovascular disease is the cause of death in ≈50% of hemodialysis patients. Accumulation of uremic solutes in systemic circulation is thought to be a key driver of the endothelial dysfunction that underlies elevated cardiovascular event…

A facile fluid pressure system reveals differential cellular response to interstitial pressure gradients and flow Open

Hao Wang, Jingming Lu, Mitesh Rathod, Wen Yih Aw, Stephanie Huang , et al. · 2023

Interstitial fluid pressure gradients and interstitial flow have been shown to drive morphogenic processes that shape tissues and influence progression of diseases including cancer. The advent of porous media microfluidic approaches has en…

Patient-derived extracellular matrix demonstrates role of COL3A1 in blood vessel mechanics Open

Elizabeth L. Doherty, Wen Yih Aw, Emily Warren, Max A. Hockenberry, Chloe P. Whitworth , et al. · 2023

Vascular Ehlers-Danlos Syndrome (vEDS) is a rare autosomal dominant disease caused by mutations in the COL3A1 gene, which renders patients susceptible to aneurysm and arterial dissection and rupture. To determine the role of COL3A1 variant…

Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation Open

Wen Yih Aw, Crescentia Cho, Hao Wang, Anne Hope Cooper, Elizabeth L. Doherty , et al. · 2023

Somatic activating mutations of PIK3CA are associated with development of vascular malformations (VMs). Here, we describe a microfluidic model of PIK3CA -driven VMs consisting of human umbilical vein endothelial cells expressing PIK3CA act…

Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization Open

Lisa E. L. Romano, Wen Yih Aw, Kathryn Hixson, T. V. Novoselova, Tammy M. Havener , et al. · 2022

Patient-derived extracellular matrix demonstrates role of COL3A1 in blood vessel mechanics Open

Elizabeth L. Doherty, Wen Yih Aw, Emily Warren, Max A. Hockenberry, Grace Krohn , et al. · 2022

Vascular Ehlers-Danlos Syndrome (vEDS) is a rare autosomal dominant disease caused by mutations in the COL3A1 gene, which renders patients susceptible to aneurysm and arterial dissection and rupture. To determine the role of COL3A1 variant…

Microphysiological vascular malformation model reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation Open

Wen Yih Aw, Crescentia Cho, Hao Wang, Anne Hope Cooper, Elizabeth L. Doherty , et al. · 2022

Somatic activating mutations of PIK3CA are associated with the development of vascular malformations (VMs). Here, we describe a microfluidic model of PIK3CA -driven VMs consisting of human umbilical vein endothelial cells (HUVECs) expressi…

Multilayer microfluidic platform for the study of luminal, transmural, and interstitial flow Open

Gi-hun Lee, Stephanie Huang, Wen Yih Aw, Mitesh Rathod, Crescentia Cho , et al. · 2022

Efficient delivery of oxygen and nutrients to tissues requires an intricate balance of blood, lymphatic, and interstitial fluid pressures (IFPs), and gradients in fluid pressure drive the flow of blood, lymph, and interstitial fluid throug…

The ataxia protein sacsin is required for integrin trafficking and synaptic organization Open

Lisa E. L. Romano, Wen Yih Aw, Kathryn Hixson, T. V. Novoselova, Tammy M. Havener , et al. · 2021

Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is caused by mutations in SACS , which manifest as a childhood-onset cerebellar ataxia. Cellular ARSACS phenotypes include mitochondrial dysfunction, intermediate filament …

Confirmation of COL4A6 variants in X-linked nonsyndromic hearing loss and its clinical implications Open

Alexander O’Brien, Wen Yih Aw, Hui Yi Tee, Kaleb M. Naegeli, Güney Bademci , et al. · 2021

Microfluidic and Organ-on-a-Chip Approaches to Investigate Cellular and Microenvironmental Contributions to Cardiovascular Function and Pathology Open

Elizabeth L. Doherty, Wen Yih Aw, Anthony J. Hickey, William J. Polacheck · 2021

Over the past decade, advances in microfabrication and biomaterials have facilitated the development of microfluidic tissue and organ models to address challenges with conventional animal and cell culture systems. These systems have largel…

An Open Science Rare Diseases Research Initiative: the University of North Carolina Catalyst Open

Kaleb M. Naegeli, Tammy M. Havener, Wen Yih Aw, Edward Anderson, D. F. C. Morris · 2019

Checking the expression and localization of split-SCN1A constructs in HEK293 cells Open

Wen Yih Aw, David Morris · 2018

Examine the functionality (expression, ability to reconstitute, and localization of re-assembled SCN1A) of split-SCN1A-GFP1-10 and split-SCN1A-GFP11 constructs

Checking the expression and localization of split-SCN1A constructs in HEK293 cells Open

Wen Yih Aw, David Morris · 2018

Examine the functionality (expression, ability to reconstitute, and localization of re-assembled SCN1A) of split-SCN1A-GFP1-10 and split-SCN1A-GFP11 constructs

Design And Cloning Of A Pair Of Split-Gfp Constructs To Examine One Of The Split-Sites For Scn1A Open

Wen Yih Aw, David Morris · 2018

Design and cloning of split-GFP constructs to investigate a potential split-sites for SCN1A fragments complementation.

Design And Cloning Of A Pair Of Split-Gfp Constructs To Examine One Of The Split-Sites For Scn1A Open

Wen Yih Aw, David Morris · 2018

Design and cloning of split-GFP constructs to investigate a potential split-sites for SCN1A fragments complementation.

Microbiome assay v1 Open

Wen Yih Aw · 2018

Cytochrome c oxidase assay v1 Open

Wen Yih Aw · 2018

Western Blot v1 Open

Wen Yih Aw · 2018

PacBio long read sequencing v1 Open

Wen Yih Aw · 2018

Fertility v1 Open

Wen Yih Aw · 2018

Oxidative stress v1 Open

Wen Yih Aw · 2018

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