Wen‐Yih Chen
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View article: The optimal ionic concentration of sensing buffer in the detection of RNA by using the DNA probe with the silicon nanowire field-effect transistor (SiNW-FET)
The optimal ionic concentration of sensing buffer in the detection of RNA by using the DNA probe with the silicon nanowire field-effect transistor (SiNW-FET) Open
This study investigates optimal conditions for surface modification and ionic concentration of sensing buffers for miRNA-21 detection using DNA probes on silicon nanowire field-effect transistor (SiNW-FET) biosensors. Ionic strength is a k…
View article: Integrating Sulfobetaine Methacrylate Hydrogel-Modified Cellulose Triacetate Filter into a Zwitterionized Tandem Membrane System for Exosome Isolation
Integrating Sulfobetaine Methacrylate Hydrogel-Modified Cellulose Triacetate Filter into a Zwitterionized Tandem Membrane System for Exosome Isolation Open
Purifying exosomes from various biotissue samples is critical for downstream analysis for diagnosis and prognosis because these extracellular vesicles not only carry enriched genetic materials but also play multiple roles in several diseas…
View article: Comparing Top-Gate and Bottom-Gate Nanowire Field-Effect Transistors on Ph Sensing with Different Salt Concentrations and Surface Modifications
Comparing Top-Gate and Bottom-Gate Nanowire Field-Effect Transistors on Ph Sensing with Different Salt Concentrations and Surface Modifications Open
View article: The Optimal Ionic Concentration of Sensing Buffer in the Detection of Rna by Using the DNA Probe with the Silicon Nanowire Field-Effect Transistor (Sinw-Fet)
The Optimal Ionic Concentration of Sensing Buffer in the Detection of Rna by Using the DNA Probe with the Silicon Nanowire Field-Effect Transistor (Sinw-Fet) Open
View article: Detecting glucose in a cell culture medium by surface-enhanced Raman scattering on InGaN quantum wells
Detecting glucose in a cell culture medium by surface-enhanced Raman scattering on InGaN quantum wells Open
Cell cultivation is a multi-billion dollar industry. The industry is currently in great need of a glucose-monitoring tool to maximize the yield of biological products. However, detecting glucose in a cell culture medium is no easy task. Th…
View article: Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study
Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study Open
Emerging evidence has shown that microRNAs play pivotal roles in wound healing. MicroRNA-21 (miR-21) was previously found to upregulate in order to fulfill an anti-inflammation role for wounds. Exosomal miRNAs have been identified and expl…
View article: Paper-based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study
Paper-based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study Open
Emerging evidence has shown that microRNAs play pivotal roles in wound healing. MicroRNA-21 (miR-21) was previously found to upregulate in order to fulfill an anti-inflammation role for wounds. Exosomal miRNAs have been identified and expl…
View article: Ultrasensitive Detection of Interleukin 6 by Using Silicon Nanowire Field-Effect Transistors
Ultrasensitive Detection of Interleukin 6 by Using Silicon Nanowire Field-Effect Transistors Open
Interleukin 6 (IL-6) has been regarded as a biomarker that can be applied as a predictor for the severity of COVID-19-infected patients. The IL-6 level also correlates well with respiratory dysfunction and mortality risk. In this work, thr…
View article: Molecular effects of site-specific phosphate-methylated primer on the structure and motions of Taq DNA polymerase
Molecular effects of site-specific phosphate-methylated primer on the structure and motions of Taq DNA polymerase Open
Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in specific gene detection. Recently, oligonucleotides contain…
View article: PLBD: protein–ligand binding database of thermodynamic and kinetic intrinsic parameters
PLBD: protein–ligand binding database of thermodynamic and kinetic intrinsic parameters Open
We introduce a protein–ligand binding database (PLBD) that presents thermodynamic and kinetic data of reversible protein interactions with small molecule compounds. The manually curated binding data are linked to protein–ligand crystal str…
View article: Sensitive and specific miRNA <i>in situ</i> hybridization using partially methylated phosphotriester antisense DNA probes
Sensitive and specific miRNA <i>in situ</i> hybridization using partially methylated phosphotriester antisense DNA probes Open
Neutralized DNA (nDNA) is an emerging class of DNA oligonucleotides chemically synthesized with site-specific internucleoside methyl phosphotriester linkages, changing the negatively charged DNA phosphodiester backbone to a neutral methyl …
View article: Real-time changes in the AC magnetic susceptibility of reagents during immunomagnetic reduction assays
Real-time changes in the AC magnetic susceptibility of reagents during immunomagnetic reduction assays Open
Antibody-functionalized magnetic nanoparticles dispersed in phosphate-buffered saline solution were used as reagents in immunomagnetic reduction assays. Biomolecules are detected in bioliquid samples when they associate with magnetic nanop…
View article: In-Silico Selection of Aptamer Targeting SARS-CoV-2 Spike Protein
In-Silico Selection of Aptamer Targeting SARS-CoV-2 Spike Protein Open
Aptamers are single-stranded, short DNA or RNA oligonucleotides that can specifically bind to various target molecules. To diagnose the infected cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in time, numerous conven…
View article: Paper-Based Devices for Capturing Exosomes and Exosomal Nucleic Acids From Biological Samples
Paper-Based Devices for Capturing Exosomes and Exosomal Nucleic Acids From Biological Samples Open
Exosomes, nanovesicles derived from cells, contain a variety of biomolecules that can be considered biomarkers for disease diagnosis, including microRNAs (miRNAs). Given knowledge and demand, inexpensive, robust, and easy-to-use tools that…
View article: Combination of Aptamer Amplifier and Antigen-Binding Fragment Probe as a Novel Strategy to Improve Detection Limit of Silicon Nanowire Field-Effect Transistor Immunosensors
Combination of Aptamer Amplifier and Antigen-Binding Fragment Probe as a Novel Strategy to Improve Detection Limit of Silicon Nanowire Field-Effect Transistor Immunosensors Open
Detecting proteins at low concentrations in high-ionic-strength conditions by silicon nanowire field-effect transistors (SiNWFETs) is severely hindered due to the weakened signal, primarily caused by screening effects. In this study, aptam…
View article: Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane
Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane Open
In clinical applications of miRNAs, the purity and quality of the testing samples are very critical, especially the obtained tissue sample volume is limited. If the extracted miRNAs are contaminated or different in quality before analysis,…
View article: Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors
Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors Open
Aptamers, in sensing technology, are famous for their role as receptors in versatile applications due to their high specificity and selectivity to a wide range of targets including proteins, small molecules, oligonucleotides, metal ions, v…
View article: Signal Enhancement of Silicon Nanowire Field-Effect Transistor Immunosensors by RNA Aptamer
Signal Enhancement of Silicon Nanowire Field-Effect Transistor Immunosensors by RNA Aptamer Open
Silicon nanowire field-effect transistors (SiNW-FETs) have been demonstrated as a highly sensitive platform for label-free detection of a variety of biological and chemical entities. However, detecting signal from immunoassays by nano-FETs…
View article: The Combination of Computational and Biosensing Technologies for Selecting Aptamer against Prostate Specific Antigen
The Combination of Computational and Biosensing Technologies for Selecting Aptamer against Prostate Specific Antigen Open
Herein, we report a method of combining bioinformatics and biosensing technologies to select aptamers against prostate specific antigen (PSA). The main objective of this study is to select DNA aptamers with higher binding affinity for PSA …
View article: A replaceable liposomal aptamer for the ultrasensitive and rapid detection of biotin
A replaceable liposomal aptamer for the ultrasensitive and rapid detection of biotin Open
View article: Computational Selection of RNA Aptamer against Angiopoietin-2 and Experimental Evaluation
Computational Selection of RNA Aptamer against Angiopoietin-2 and Experimental Evaluation Open
Angiogenesis plays a decisive role in the growth and spread of cancer and angiopoietin-2 (Ang2) is in the spotlight of studies for its unique role in modulating angiogenesis. The aim of this study was to introduce a computational simulatio…