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View article: Possible Rethink of the Bacterial Peptidoglycan Layer as a Non-Porous Layer Rather Than a Perforated Mesh
Possible Rethink of the Bacterial Peptidoglycan Layer as a Non-Porous Layer Rather Than a Perforated Mesh Open
In Gram-negative bacteria, the peptidoglycan layer is often thought to be a thin layer (even monolayer) that is in-between the two phospholipid bilayer of the bacterial double membrane cell envelope. However, preliminary evidence from scan…
View article: Exploring Consumer Preferences: Organic vs. Non-Organic Body Wash Brands in Malaysia
Exploring Consumer Preferences: Organic vs. Non-Organic Body Wash Brands in Malaysia Open
The COVID-19 pandemic has heightened consumer awareness of personal hygiene, leading to a surge in demand for bath and shower products in Malaysia. Despite this growth, the organic body wash segment has faced challenges, including consumer…
View article: Statistical and Correlative Analysis of Ribosomal Proteins of <em>Escherichia coli</em>
Statistical and Correlative Analysis of Ribosomal Proteins of <em>Escherichia coli</em> Open
Ribosome is the macromolecular machine that regenerates cells, and support life through continuous protein synthesis. It is an adaptable machine able to synthesize proteins at different speed and production volume depending on the strength…
View article: A Content Analysis of Digital Marketing Strategies of Formula Companies and Influencers to Promote Commercial Milk Formula in Hong Kong
A Content Analysis of Digital Marketing Strategies of Formula Companies and Influencers to Promote Commercial Milk Formula in Hong Kong Open
This study examines the compliance of the digital marketing strategies used by formula companies and social media influencers in promoting commercial milk formula (CMF) for infants and young children with the International Code of Marketin…
View article: Twenty Amino Acids Random Peptide Yields BLASTp Hits with Good Percent Identity
Twenty Amino Acids Random Peptide Yields BLASTp Hits with Good Percent Identity Open
View article: Theoretical 2D protein gel of Helicobacter pylori
Theoretical 2D protein gel of Helicobacter pylori Open
View article: Estimation of infected area and its severity in chest X-ray images of tuberculosis patients
Estimation of infected area and its severity in chest X-ray images of tuberculosis patients Open
Despite much research, tuberculosis (TB) remains a significant health burden in both developing and advanced countries. Diagnosis of tuberculosis still rely on chest X-ray images which are interpreted qualitatively by radiologist. Hence, m…
View article: Detection of Two Phylogenetic Clusters in Helicobacter pylori 23S rRNA Phylogeny Suggests Emergence of Two Distinct Phylotype of the Species
Detection of Two Phylogenetic Clusters in Helicobacter pylori 23S rRNA Phylogeny Suggests Emergence of Two Distinct Phylotype of the Species Open
Phylogenetic tree analysis is commonly used to understand the evolutionary distance between different species or strains of the same species. This work originally aimed to gain an understanding and visual depiction of the evolutionary dist…
View article: Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry
Human KRAS Gene May Be Hard to Detect in Quantitative PCR via Probe Detection Chemistry Open
Quantitative polymerase chain reaction (qPCR) is the gold standard approach for detecting a variety of pathogens either via probe-based or SYBR green detection chemistry. Generally, probe-based detection is more specific than SYBR green ch…
View article: Effect of template and primer-probe concentration level on shape of qPCR amplification curve
Effect of template and primer-probe concentration level on shape of qPCR amplification curve Open
Quantitative polymerase chain reaction (qPCR) is the gold standard approach for the sensitive detection of a variety of pathogens in the bacterial genre. This work sought to understand the effect of template concentration and primer and pr…
View article: Emergence of small amount of organic leaching during copper sorption on treated seaweed under ammonium interference
Emergence of small amount of organic leaching during copper sorption on treated seaweed under ammonium interference Open
Biosorbents have been explored as potential adsorbents for wastewater treatment for the past two decades. One of the hindrances hampering their commercial use in wastewater treatment is organic leaching from the biosorbent during sorption …
View article: No tight correlation between transcription and translation for highly expressed genes in Pseudomonas fluorescens
No tight correlation between transcription and translation for highly expressed genes in Pseudomonas fluorescens Open
Transcription is commonly assumed to correlate tightly with translation. Thus, protein level of an enzyme is similar to its mRNA transcript level, which opens up the use of RNA-seq transcriptomics as a proxy for probing gene expression pat…
View article: Relative lack of diversity of microbes recovered on LB Miller agar medium
Relative lack of diversity of microbes recovered on LB Miller agar medium Open
Colour of microbial colonies is the first observational clue to microbial identity and type. Although unable to offer much use in microbial classification, diversity of pigmented coloured colonies or lack of on an agar plate still offers u…
View article: Difficulty in Achieving High Identification Accuracy and High Amplification Efficiency in Bacterial 16s rRNA Amplicon Metagenomics
Difficulty in Achieving High Identification Accuracy and High Amplification Efficiency in Bacterial 16s rRNA Amplicon Metagenomics Open
Bacterial phylogenetics has largely been determined via 16S rRNA gene sequencing and phylogenetic tree reconstruction. Observed utility of this approach has driven the popularity of the 16S rRNA gene amplicon metagenomics method for profil…
View article: Single phase biotransformation of acetophenone to 1-phenylethanol using recombinant Escherichia coli expressing cpsADH alcohol dehydrogenase
Single phase biotransformation of acetophenone to 1-phenylethanol using recombinant Escherichia coli expressing cpsADH alcohol dehydrogenase Open
Biotransformation of organic substrates into organic products typically perform better with a two-phase system where the organic solvent such as n-hexadecane helps to solubilize the substrate and product. This work sought to test out the a…
View article: Recent Developments in High Spatio-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications
Recent Developments in High Spatio-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications Open
Dawn of the omics revolution in biological sciences meant that we seek to understand more and in greater detail the molecular constituents of cells and biological systems. While we have gained significant insights from conventional omics t…
View article: Understanding the Different Parameters That Affects Bacterial Transformation Efficiency
Understanding the Different Parameters That Affects Bacterial Transformation Efficiency Open
Bacterial transformation is the essential tool for introducing foreign DNA into bacterial cells in a host of recombinant technology applications useful for biomanufacturing and drug production. Thus far, chemically competent cells remain a…
View article: Metrics for Understanding the Degraded Metagenome of Stomach Cancer Patient
Metrics for Understanding the Degraded Metagenome of Stomach Cancer Patient Open
Metagenomics have opened our eyes to the otherwise enigmatic microbial consortia that relies on different human body niches that impact on disease pathogenesis. This work originally aims to re-analyse a public shotgun metagenomics dataset …
View article: Technological Innovations in COVID-19 Diagnostics in Singapore
Technological Innovations in COVID-19 Diagnostics in Singapore Open
Due to species barriers and poor adaptability to new host environments, few pathogens cause global pandemics. But, SARS-CoV-2 is one exception with its high transmissivity and delayed onset of symptoms. Fortunately, the world was able to t…
View article: Distribution of number of genes in different bacteriophages
Distribution of number of genes in different bacteriophages Open
Advent of low-cost, relatively fast DNA sequencing has opened up applications in genome sequencing. Besides benefiting sequencing of whole genomes of bacteria, archaea and fungi, it has also lend its weight to improve our understanding of …
View article: Theoretical analysis suggests that variants of 16S rRNA gene of Helicobacter pylori could not be differentiated by PCR melt curve analysis of amplicons
Theoretical analysis suggests that variants of 16S rRNA gene of Helicobacter pylori could not be differentiated by PCR melt curve analysis of amplicons Open
Possibility exists that qPCR primers could bind to different variants of template in the sample, thereby, leading to the amplifications of different variants and generations of amplicons slightly differentiated in sequence. Melt curve anal…
View article: Shear stress likely reduced viability of Escherichia coli cells as they pass through a narrow constriction in PMMA microfluidic chip
Shear stress likely reduced viability of Escherichia coli cells as they pass through a narrow constriction in PMMA microfluidic chip Open
Common to many microfluidic chips is narrow constriction as inlet and outlets which need to be accessed by needles. Part of the reason for the use of these narrow constriction is to reduce sample evaporation. However, would narrow constric…
View article: <strong>Dataset of DNA microarray probe for every gene in the genome of </strong><em><strong>Escherichia coli</strong></em><strong> K-12</strong>
<strong>Dataset of DNA microarray probe for every gene in the genome of </strong><em><strong>Escherichia coli</strong></em><strong> K-12</strong> Open
Prior to the maturation of RNA-seq technology, DNA microarray is the go-to tool for understanding the genome-level gene expression pattern of particular organism. However, the probes used to fish out the mRNA are often designed by commerci…
View article: Recent Developments in High Spatial-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications
Recent Developments in High Spatial-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications Open
Dawn of the omics revolution in biological sciences meant that we seek to understand more and in greater detail the molecular constituents of cells and biological systems. While we have gained significant insights from conventional omics t…
View article: Candidate microRNA therapeutics for genes in the SARS-CoV-2 genome
Candidate microRNA therapeutics for genes in the SARS-CoV-2 genome Open
Through supressing protein translation, microRNA offers a route for serving as therapeutics for a variety of bacterial, viral, and fungal pathogens. Specifically, microRNA could bind to the mRNA transcripts of genes of these pathogens to s…
View article: Phylogeny of Bacillus Subtilis Strains Cannot Be Resolved by 16S rRNA Due to Very Close Evolutionary Distance in the Gene
Phylogeny of Bacillus Subtilis Strains Cannot Be Resolved by 16S rRNA Due to Very Close Evolutionary Distance in the Gene Open
View article: Partial re-analysis of upper respiratory tract microbiome of COVID-19 positive patient reveals diverse microbial species
Partial re-analysis of upper respiratory tract microbiome of COVID-19 positive patient reveals diverse microbial species Open
Understanding the microbiome of the respiratory tract of COVID-19 positive patient is important for identifying any possible microbial co-infectant of the disease that can result in severe disease. With this aim in mind, this study underto…
View article: Synthetic positive control for human KRAS gene
Synthetic positive control for human KRAS gene Open
Synthetic positive control has emerged as the essential tool for modern qPCR testing kit as a means for the clinicians to verify that the test performs correctly. With declining cost of gene synthesis, more clinical diagnostic companies ar…
View article: Examining the Kinetics of E. Coli Whole Cell Biotransformation of Acetophenone to 1-Phenylethanol
Examining the Kinetics of E. Coli Whole Cell Biotransformation of Acetophenone to 1-Phenylethanol Open
View article: Small compilation of articles on cofactor engineering approaches in biocatalysis
Small compilation of articles on cofactor engineering approaches in biocatalysis Open
Given that many enzymes in biocatalysis require redox equivalents such as NADH or FADH2, it is critical to engineer pathways or methods for regenerating these redox equivalents as their stoichiometric provision in biocatalytic reactions is…