William B. Lott
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View article: Discrimination of Methionine Sulfoxide and Sulfone by Human Neutrophil Elastase
Discrimination of Methionine Sulfoxide and Sulfone by Human Neutrophil Elastase Open
Human neutrophil elastase (HNE) is a uniquely destructive serine protease with the ability to unleash a wave of proteolytic activity by destroying the inhibitors of other proteases. Although this phenomenon forms an important part of the i…
View article: Transforming growth factor-beta stimulates human bone marrow-derived mesenchymal stem/stromal cell chondrogenesis more so than kartogenin
Transforming growth factor-beta stimulates human bone marrow-derived mesenchymal stem/stromal cell chondrogenesis more so than kartogenin Open
A previous study identified kartogenin (KGN) as a potent modulator of bone marrow mesenchymal stem/stromal cell (BMSC) chondrogenesis. This initial report did not contrast KGN directly against transforming growth factor-beta 1 (TGF-β1), th…
Modelling mesenchymal stromal cell growth in a packed bed bioreactor with a gas permeable wall Open
A mathematical model was developed for mesenchymal stromal cell (MSC) growth in a packed bed bioreactor that improves oxygen availability by allowing oxygen diffusion through a gas-permeable wall. The governing equations for oxygen, glucos…
View article: Spheroid Coculture of Hematopoietic Stem/Progenitor Cells and Monolayer Expanded Mesenchymal Stem/Stromal Cells in Polydimethylsiloxane Microwells Modestly Improves<i>In Vitro</i>Hematopoietic Stem/Progenitor Cell Expansion
Spheroid Coculture of Hematopoietic Stem/Progenitor Cells and Monolayer Expanded Mesenchymal Stem/Stromal Cells in Polydimethylsiloxane Microwells Modestly Improves<i>In Vitro</i>Hematopoietic Stem/Progenitor Cell Expansion Open
While two-dimensional (2D) monolayers of mesenchymal stem/stromal cells (MSCs) have been shown to enhance hematopoietic stem/progenitor cell (HSPC) expansion in vitro, expanded cells do not engraft long term in human recipients. This outco…
View article: Can video improve grant review quality and lead to more reliable ranking?
Can video improve grant review quality and lead to more reliable ranking? Open
Multimedia video is rapidly becoming mainstream, and many studies indicate that it is a more effective communication medium than text. In this project we AIM to test if videos can be used, in place of text-based grant proposals, to improve…
View article: Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue Open
Mesenchymal stem/stromal cells (MSC) are promising candidates for use in cell-based therapies. In most cases, therapeutic response appears to be cell-dose dependent. Human term placenta is rich in MSC and is a physically large tissue that …
View article: Direct bone marrow HSC transplantation enhances local engraftment at the expense of systemic engraftment in NSG mice
Direct bone marrow HSC transplantation enhances local engraftment at the expense of systemic engraftment in NSG mice Open
Direct bone marrow (BM) injection has been proposed as a strategy to bypass homing inefficiencies associated with intravenous (IV) hematopoietic stem cell (HSC) transplantation. Despite physical delivery into the BM cavity, many donor cell…
View article: Polydimethylsiloxane (PDMS) modulates CD38 expression, absorbs retinoic acid and may perturb retinoid signalling
Polydimethylsiloxane (PDMS) modulates CD38 expression, absorbs retinoic acid and may perturb retinoid signalling Open
All-trans retinoic acid (ATRA) is absorbed by PDMS and depleted from culture media, influencing gene expression and phenotype across a range of cell types.
View article: Polydimethylsiloxane (PDMS) modulates CD38 expression, absorbs retinoic acid and may perturb retinoid signalling
Polydimethylsiloxane (PDMS) modulates CD38 expression, absorbs retinoic acid and may perturb retinoid signalling Open
Polydimethylsiloxane (PDMS) is the most commonly used material in the manufacture of customized cell culture devices. While there is concern that uncured PDMS oligomers may leach into culture medium and/or hydrophobic molecules may be abso…