William Comstock
YOU?
Author Swipe
View article: Profiling Tel1 signaling reveals a non-canonical motif targeting DNA repair and telomere control machineries
Profiling Tel1 signaling reveals a non-canonical motif targeting DNA repair and telomere control machineries Open
The stability of the genome relies on phosphatidyl inositol 3-kinase-related kinases (PIKKs) that sense DNA damage and trigger elaborate downstream signaling responses. In Saccharomyces cerevisiae, the Tel1 kinase (ortholog of human ATM) i…
View article: Proteomic Sensors for Quantitative, Multiplexed and Spatial Monitoring of Kinase Signaling
Proteomic Sensors for Quantitative, Multiplexed and Spatial Monitoring of Kinase Signaling Open
Understanding kinase action requires precise quantitative measurements of their activity in vivo . In addition, the ability to capture spatial information of kinase activity is crucial to deconvolute complex signaling networks, interrogate…
View article: A TOPBP1 allele causing male infertility uncouples XY silencing dynamics from sex body formation
A TOPBP1 allele causing male infertility uncouples XY silencing dynamics from sex body formation Open
Meiotic sex chromosome inactivation (MSCI) is a critical feature of meiotic prophase I progression in males. While the ATR kinase and its activator TOPBP1 are key drivers of MSCI within the specialized sex body (SB) domain of the nucleus, …
View article: Author Response: A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation
Author Response: A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation Open
Meiotic sex chromosome inactivation (MSCI) is a critical feature of meiotic prophase I progression in males. While the ATR kinase and its activator TOPBP1 are key drivers of MSCI within the specialized sex body (SB) domain of the nucleus, …
View article: A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation
A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation Open
Meiotic sex chromosome inactivation (MSCI) is a critical feature of meiotic prophase I progression in males. While the ATR kinase and its activator TOPBP1 are key drivers of MSCI within the specialized sex body (SB) domain of the nucleus, …
View article: Reviewer #1 (Public Review): A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation
Reviewer #1 (Public Review): A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation Open
Meiotic sex chromosome inactivation (MSCI) is a critical feature of meiotic prophase I progression in males. While the ATR kinase and its activator TOPBP1 are key drivers of MSCI within the specialized sex body (SB) domain of the nucleus, …
View article: A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation
A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation Open
Meiotic sex chromosome inactivation (MSCI) is a critical feature of meiotic prophase I progression in males. While the ATR kinase and its activator TOPBP1 are key drivers of MSCI within the specialized sex body (SB) domain of the nucleus, …
View article: A TOPBP1 allele causing male infertility uncouples XY silencing dynamics from sex body formation
A TOPBP1 allele causing male infertility uncouples XY silencing dynamics from sex body formation Open
Meiotic sex chromosome inactivation (MSCI) is a critical feature of meiotic prophase I progression in males. While the ATR kinase and its activator TOPBP1 are key drivers of MSCI within the specialized sex body (SB) domain of the nucleus, …
View article: A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation
A TOPBP1 Allele Causing Male Infertility Uncouples XY Silencing Dynamics From Sex Body Formation Open
Meiotic sex chromosome inactivation (MSCI) is a critical feature of meiotic prophase I progression in males. While the ATR kinase and its activator TOPBP1 are key drivers of MSCI within the specialized sex body (SB) domain of the nucleus, …
View article: Maximized quantitative phosphoproteomics allows high confidence dissection of the DNA damage signaling network
Maximized quantitative phosphoproteomics allows high confidence dissection of the DNA damage signaling network Open
The maintenance of genomic stability relies on DNA damage sensor kinases that detect DNA lesions and phosphorylate an extensive network of substrates. The Mec1/ATR kinase is one of the primary sensor kinases responsible for orchestrating D…
View article: Phosphoproteomics Reveals a Distinct Mode of Mec1/ATR Signaling in Response to DNA End Hyper-Resection
Phosphoproteomics Reveals a Distinct Mode of Mec1/ATR Signaling in Response to DNA End Hyper-Resection Open
The Mec1/ATR kinase is crucial for genome maintenance in response to a range of genotoxic insults, although how it promotes context-dependent signaling and DNA repair remains elusive. Here we uncovered a specialized mode of Mec1/ATR signal…
View article: Maximizing Quantitative Phosphoproteomics of Kinase Signaling Expands the Mec1 and Tel1 Networks
Maximizing Quantitative Phosphoproteomics of Kinase Signaling Expands the Mec1 and Tel1 Networks Open
Global phosphoproteome analysis is crucial for comprehensive and unbiased investigation of kinase-mediated signaling. However, since each phosphopeptide represents a unique entity for defining identity, site-localization, and quantitative …
View article: Molecular and Microbial Microenvironments in Chronically Diseased Lungs Associated with Cystic Fibrosis
Molecular and Microbial Microenvironments in Chronically Diseased Lungs Associated with Cystic Fibrosis Open
Microbial infections are now recognized to be polymicrobial and personalized in nature. Comprehensive analysis and understanding of the factors underlying the polymicrobial and personalized nature of infections remain limited, especially i…
View article: The Molecular and Microbial Microenvironments in Chronically Diseased Lungs
The Molecular and Microbial Microenvironments in Chronically Diseased Lungs Open
To visualize the personalized distributions of pathogens, chemical environments including microbial metabolites, pharmaceuticals, and their metabolic products within and between human lungs afflicted with cystic fibrosis, we generated 3D m…
View article: Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome
Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome Open
In cases of severe CF lung disease, proteolysis by host enzymes creates an amino acid-rich environment that P. aeruginosa comes to dominate, which may contribute to the pathogen's persistence by providing its preferred carbon source.
View article: Additional file 5: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome
Additional file 5: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome Open
Table S2. Correlations between metabolites and 16S rRNA gene OTUs with Target FEV1%. (XLSX 438 kb)
View article: Additional file 3: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome
Additional file 3: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome Open
Table S6. 16S rRNA gene read abundance for sputum samples and blanks after illumina sequencing. (XLSX 43 kb)
View article: Additional file 8: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome
Additional file 8: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome Open
Table S5. p values from complete dataset and independent dataset (1 sample each patient) for Mann-Whitney U tests of different variables in this study. (XLSX 9 kb)
View article: Additional file 7: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome
Additional file 7: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome Open
Table S4. Human and Pseudomonas aeruginosa peptides from MS-GF+ proteomics search against human and P. aeruginosa SwissProt genomes. (XLSX 103 kb)
View article: Additional file 6: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome
Additional file 6: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome Open
Table S3. Peptidomics data on human proteome as produced using PEAKS software. (XLSX 87 kb)
View article: Additional file 1: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome
Additional file 1: of Neutrophilic proteolysis in the cystic fibrosis lung correlates with a pathogenic microbiome Open
Table S1. Samples and clinical information for patients included in this study. (XLSX 56 kb)
View article: Niche partitioning of a pathogenic microbiome driven by chemical gradients
Niche partitioning of a pathogenic microbiome driven by chemical gradients Open
Chemical gradients shape the cystic fibrosis lung microbiome, with marked effects on the outcome of antimicrobial treatments.