William D. Bedzyk
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View article: Impact of Antibody Specificity and Calibration Material on the Measure of Agreement between Methods for Cardiac Troponin I
Impact of Antibody Specificity and Calibration Material on the Measure of Agreement between Methods for Cardiac Troponin I Open
Background: Available assays for cardiac troponin I (cTnI) yield numerically different results. The aim of this study was to compare patient values obtained from four cTnI immunoassays. Methods: We studied the Stratus® II assay, the Opus® …
View article: Development and validation of an automated latex-enhanced immunoassay for prealbumin
Development and validation of an automated latex-enhanced immunoassay for prealbumin Open
The measurement of circulating prealbumin has been shown to be clinically useful in the assessment of nutritional status, both as an initial screen and in the monitoring of nutritional recovery. We describe a fully automated, noncompetitiv…
View article: Molecular mass determination for prostate‐specific antigen and α<sub>1</sub>‐antichymotrypsin complexed in vitro
Molecular mass determination for prostate‐specific antigen and α<sub>1</sub>‐antichymotrypsin complexed in vitro Open
The results from molecular mass determinations and amino acid analyses for prostate‐specific antigen (PSA), α 1 ‐antichymotrypsin (ACT) and PSA‐ACT complexed in vitro are reported. Molecular masses for two separate PSA‐ACT lots were determ…
View article: Conversion of an anti-single-stranded DNA active site to an anti-fluorescein active site through heavy chain complementarity determining region transplantation.
Conversion of an anti-single-stranded DNA active site to an anti-fluorescein active site through heavy chain complementarity determining region transplantation. Open
Complementarity determining region (CDR) transplant studies were conducted between two monoclonal antibodies of distinctly different specificities (anti-fluorescein monoclonal antibody (mAb) 4-4-20 and anti-single-stranded DNA (ssDNA) mAb …
View article: Author Index
Author Index Open
Anti-metatype antibodies stabilize the fluorescein sinby hydrostatic pressure
View article: Immunological and structural characterization of a high affinity anti-fluorescein single-chain antibody.
Immunological and structural characterization of a high affinity anti-fluorescein single-chain antibody. Open
Single-chain antibody of the (NH2) VL-linker-VH (COOH) design, was constructed based on prototype high affinity anti-fluorescein monoclonal antibody (mAb) 4-4-20. Purified single-chain antibody (SCA) 4-4-20/212 was studied relative to Ig m…
View article: Active site structure and antigen binding properties of idiotypically cross-reactive anti-fluorescein monoclonal antibodies.
Active site structure and antigen binding properties of idiotypically cross-reactive anti-fluorescein monoclonal antibodies. Open
This report includes complete VH and V kappa nucleotide and deduced amino acid sequences of idiotypically cross-reactive monoclonal anti-fluorescein antibodies that differed greater than 10(5)-fold in affinity. High affinity monoclonal ant…
View article: Variable region primary structures of a high affinity anti-fluorescein immunoglobulin M cryoglobulin exhibiting oxazolone cross-reactivity
Variable region primary structures of a high affinity anti-fluorescein immunoglobulin M cryoglobulin exhibiting oxazolone cross-reactivity Open
Previous studies of murine IgM hybridoma protein 18-2-3, derived from an (NZB/NZW)F1 mouse following hyperimmunization with fluorescein (Fl)-conjugated keyhole limpet hemocyanin, demonstrated a high affinity for Fl (Ka = 2.9 x 10(10) M-1) …
View article: Comparison of Variable Region Primary Structures within an Anti-fluorescein Idiotype Family
Comparison of Variable Region Primary Structures within an Anti-fluorescein Idiotype Family Open
Previous reports described the properties of a high affinity (Ka = 1.7 X 10(10) M-1) prototype anti-fluorescein monoclonal antibody 4-4-20, an intermediate affinity (Ka = 3.7 X 10(7) M-1) prototype 9-40, and Ig members of the 9-40 idiotype…