William M. Schneider
YOU?
Author Swipe
View article: An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaks
An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaks Open
Retrons are a retroelement class found in diverse prokaryotes that can be adapted to augment CRISPR–Cas9 genome engineering technology to efficiently rewrite short stretches of genetic information in bacteria and yeast. However, efficiency…
View article: A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist
A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist Open
Interferons (IFNs) play a crucial role in the regulation and evolution of host–virus interactions. Here, we conducted a genome-wide arrayed CRISPR knockout screen in the presence and absence of IFN to identify human genes that influence Se…
View article: An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaks
An enhanced Eco1 retron editor enables precision genome engineering in human cells without double-strand breaks Open
Retrons are a retroelement class found in diverse prokaryotes that can be adapted to augment CRISPR-Cas9 genome engineering technology to efficiently rewrite short stretches of genetic information in bacteria and yeast. However, efficiency…
View article: Deep mutational scanning of hepatitis B virus reveals a mechanism for cis-preferential reverse transcription
Deep mutational scanning of hepatitis B virus reveals a mechanism for cis-preferential reverse transcription Open
View article: A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist
A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist Open
Interferons (IFNs) play a crucial role in the regulation and evolution of host-virus interactions. Here, we conducted a genome-wide arrayed CRISPR knockout screen in the presence and absence of IFN to identify human genes that influence SA…
View article: An RNA-based system to study hepatitis B virus replication and evaluate antivirals
An RNA-based system to study hepatitis B virus replication and evaluate antivirals Open
Hepatitis B virus (HBV) chronically infects an estimated 300 million people, and standard treatments are rarely curative. Infection increases the risk of liver cirrhosis and hepatocellular carcinoma, and consequently, nearly 1 million peop…
View article: <scp>DDX60</scp> selectively reduces translation off viral type <scp>II</scp> internal ribosome entry sites
<span>DDX60</span> selectively reduces translation off viral type <span>II</span> internal ribosome entry sites Open
View article: Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice
Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice Open
View article: Antiviral DExD/H-box helicase 60 selectively inhibits translation from type II internal ribosome entry sites
Antiviral DExD/H-box helicase 60 selectively inhibits translation from type II internal ribosome entry sites Open
During viral infection, competition ensues between viruses and their host cells to control the protein synthesis machinery. In response, certain host defense proteins globally limit mRNA translation. However, this is also detrimental for h…
View article: Flavivirus–host interactions: an expanding network of proviral and antiviral factors
Flavivirus–host interactions: an expanding network of proviral and antiviral factors Open
View article: A CRISPR Activation Screen Identifies an Atypical Rho GTPase That Enhances Zika Viral Entry
A CRISPR Activation Screen Identifies an Atypical Rho GTPase That Enhances Zika Viral Entry Open
Zika virus (ZIKV) is a re-emerging flavivirus that has caused large-scale epidemics. Infection during pregnancy can lead to neurologic developmental abnormalities in children. There is no approved vaccine or therapy for ZIKV. To uncover ce…
View article: Replication and single-cycle delivery of SARS-CoV-2 replicons
Replication and single-cycle delivery of SARS-CoV-2 replicons Open
A tool to study SARS-CoV-2 Work with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires high-level biocontainment facilities, making it important to develop safer molecular tools that can potentially be used u…
View article: Decoupling expression and editing preferences of ADAR1 p150 and p110 isoforms
Decoupling expression and editing preferences of ADAR1 p150 and p110 isoforms Open
Significance ADAR1, an A-to-I RNA editing enzyme, is an essential gene and also an attractive target for cancer therapy. Mutations in ADAR1 cause devastating autoinflammatory diseases, and inhibition of ADAR1 remarkably reduces tumor growt…
View article: Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors
Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors Open
View article: Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks
Genome-Scale Identification of SARS-CoV-2 and Pan-coronavirus Host Factor Networks Open
View article: TMEM41B Is a Pan-flavivirus Host Factor
TMEM41B Is a Pan-flavivirus Host Factor Open
View article: Human hepatocyte PNPLA3 148M exacerbates rapid non-alcoholic steatohepatitis development in chimeric mice
Human hepatocyte PNPLA3 148M exacerbates rapid non-alcoholic steatohepatitis development in chimeric mice Open
Advanced non-alcoholic fatty liver disease (NAFLD) is a rapidly emerging global health problem associated with pre-disposing genetic polymorphisms, most strikingly an isoleucine to methionine substitution in patatin-like phospholipase doma…
View article: TMEM41B is a pan-flavivirus host factor
TMEM41B is a pan-flavivirus host factor Open
SUMMARY Flaviviruses pose a constant threat to human health. These RNA viruses are transmitted by the bite of infected mosquitoes and ticks and regularly cause outbreaks. To identify host factors required for flavivirus infection we perfor…
View article: Genome-scale identification of SARS-CoV-2 and pan-coronavirus host factor networks
Genome-scale identification of SARS-CoV-2 and pan-coronavirus host factor networks Open
SUMMARY The COVID-19 pandemic has claimed the lives of more than one million people worldwide. The causative agent, SARS-CoV-2, is a member of the Coronaviridae family, which are viruses that cause respiratory infections of varying severit…
View article: Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Inborn errors of type I IFN immunity in patients with life-threatening COVID-19 Open
The genetics underlying severe COVID-19 The immune system is complex and involves many genes, including those that encode cytokines known as interferons (IFNs). Individuals that lack specific IFNs can be more susceptible to infectious dise…
View article: Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors
Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors Open
SUMMARY The ongoing SARS-CoV-2 pandemic has devastated the global economy and claimed nearly one million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronavir…
View article: A Combination of Human Broadly Neutralizing Antibodies against Hepatitis B Virus HBsAg with Distinct Epitopes Suppresses Escape Mutations
A Combination of Human Broadly Neutralizing Antibodies against Hepatitis B Virus HBsAg with Distinct Epitopes Suppresses Escape Mutations Open
View article: Stem cell-derived polarized hepatocytes
Stem cell-derived polarized hepatocytes Open
Human stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver biology. Despite their numerous advantages, HLCs lack critical in vivo characteristics, including cell polarity. Here, we report a stem cell d…
View article: Public broadly neutralizing antibodies against hepatitis B virus in individuals with elite serologic activity
Public broadly neutralizing antibodies against hepatitis B virus in individuals with elite serologic activity Open
SUMMARY Although there is no effective cure for chronic hepatitis B virus (HBV) infection, antibodies are protective and constitute clinical correlates of recovery from infection. To examine the human neutralizing antibody response to HBV …
View article: Expansion, in vivo–ex vivo cycling, and genetic manipulation of primary human hepatocytes
Expansion, in vivo–ex vivo cycling, and genetic manipulation of primary human hepatocytes Open
Primary human hepatocytes (PHHs) are an essential tool for modeling drug metabolism and liver disease. However, variable plating efficiencies, short lifespan in culture, and resistance to genetic manipulation have limited their use. Here, …
View article: Expansion, in vivo–ex vivo cycling, and genetic manipulation of primary human hepatocytes
Expansion, in vivo–ex vivo cycling, and genetic manipulation of primary human hepatocytes Open
© 2020 National Academy of Sciences. All rights reserved. Primary human hepatocytes (PHHs) are an essential tool for modeling drug metabolism and liver disease. However, variable plating efficiencies, short lifespan in culture, and resista…
View article: An RNA-based system to study hepatitis B virus replication and select drug-resistance mutations
An RNA-based system to study hepatitis B virus replication and select drug-resistance mutations Open
Hepatitis B virus (HBV) chronically infects over 250 million people worldwide, increasing their risk of liver cirrhosis and hepatocellular carcinoma. There is a vaccine to prevent new infections, but no efficient cure for chronic infection…
View article: Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP)
Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP) Open
ZAP is an IFN-induced host factor that can inhibit a wide range of viruses, and there is great interest in fully characterizing its antiviral mechanism. This is the first study that defines the antiviral capacities of individual ZAP isofor…
View article: Intrinsic Immunity Shapes Viral Resistance of Stem Cells
Intrinsic Immunity Shapes Viral Resistance of Stem Cells Open
View article: A robust cell culture system supporting the complete life cycle of hepatitis B virus
A robust cell culture system supporting the complete life cycle of hepatitis B virus Open
The discovery of sodium taurocholate cotransporting polypeptide (NTCP) as the hepatitis B virus (HBV) receptor enabled researchers to create hepatoma cell lines susceptible to HBV infection. Infection in current systems, however, is ineffi…