Wim de Lau
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View article: Controlling Intestinal Organoid Polarity using Synthetic Dynamic Hydrogels Decorated with Laminin‐Derived IKVAV Peptides
Controlling Intestinal Organoid Polarity using Synthetic Dynamic Hydrogels Decorated with Laminin‐Derived IKVAV Peptides Open
Intestinal organoids are three‐dimensional cellular structures that are cultured in laminin‐rich Matrigel, yielding organoids with correct, basal‐out polarity. Removal of Matrigel results in organoids with reversed, apical‐out polarity, de…
View article: Integrin-activating <i>Yersinia</i> protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets
Integrin-activating <i>Yersinia</i> protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets Open
Matrigel ® /BME ® , a basement membrane-like preparation, supports long-term growth of epithelial 3D organoids from adult stem cells [T. Sato et al. , Nature 459 , 262–265 (2009); T. Sato et al. , Gastroenterology 141 , 1762–1772 (2011)]. …
View article: Integrin-activating Yersinia protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets
Integrin-activating Yersinia protein Invasin sustains long-term expansion of primary epithelial cells as 2D organoid sheets Open
Matrigel/BME, a basement membrane-like preparation, supports long-term growth of epithelial 3D organoids from adult stem cells (ASC) 1,2 . Here, we show that interaction between Matrigel’s major component Laminin111 with epithelial α6β1-in…
View article: A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses
A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses Open
Rapid identification of host genes essential for virus replication may expedite the generation of therapeutic interventions. Genetic screens are often performed in transformed cell lines that poorly represent viral target cells in vivo, le…
View article: Syndecan-1 promotes Wnt/β-catenin signaling in multiple myeloma by presenting Wnts and R-spondins
Syndecan-1 promotes Wnt/β-catenin signaling in multiple myeloma by presenting Wnts and R-spondins Open
Key Points HS chains decorating syndecan-1 promote autocrine and paracrine Wnt signaling in MM. Loss of HS inhibits MM cell growth by attenuating Wnt signaling.
View article: Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins
Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins Open
Significance Multiple myeloma (MM) cells are highly dependent on signals provided by the bone marrow (BM) niche for growth and survival. Most MMs display hallmarks of active Wnt signaling, but lack activating Wnt-pathway mutations, suggest…