Dae Won Moon
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View article: Imaging lipid rafts reveals the principle of ApoE4-induced Aβ upregulation in human neurons
Imaging lipid rafts reveals the principle of ApoE4-induced Aβ upregulation in human neurons Open
Lipid rafts in plasma membranes are thought to provide a platform for regulating signaling pathways by increasing the expression or proximity of proteins in the same pathway. Despite this understanding, the absence of direct, simultaneous …
View article: B-050 A Versatile Immunoassay Platform for Fast Multiplex Detection of Protein Biomarkers Based on A Novel Magnetic Particle System
B-050 A Versatile Immunoassay Platform for Fast Multiplex Detection of Protein Biomarkers Based on A Novel Magnetic Particle System Open
Background A major challenge of clinical diagnostic applications in the field of emergency department is the not only fast but also accurate measurement of multiple protein biomarkers with broad dynamic range spectrum. Here, we describe th…
View article: APOE4-carrying human astrocytes oversupply cholesterol to promote neuronal lipid raft expansion and Aβ generation
APOE4-carrying human astrocytes oversupply cholesterol to promote neuronal lipid raft expansion and Aβ generation Open
The ε4 allele of APOE-encoding apolipoprotein (ApoE) is one of the strongest genetic risk factors for Alzheimer's disease (AD). One of the overarching questions is whether and how this astrocyte-enriched risk factor initiates AD-associated…
View article: Screening of important metabolites and <scp>KRAS</scp> genotypes in colon cancer using secondary ion mass spectrometry
Screening of important metabolites and <span>KRAS</span> genotypes in colon cancer using secondary ion mass spectrometry Open
Time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) is an imaging‐based analytical technique that can characterize the surfaces of biomaterials. We used TOF‐SIMS to identify important metabolites and oncogenic KRAS mutation expressed…
View article: Distinct roles of stereociliary links in the nonlinear sound processing and noise resistance of cochlear outer hair cells
Distinct roles of stereociliary links in the nonlinear sound processing and noise resistance of cochlear outer hair cells Open
Significance Our hearing organ, the cochlea, acts as an active sound amplifier rather than a simple detector due to the action of outer hair cells (OHCs). This active sound processing by OHCs requires specific hair bundle architecture in w…
View article: Interhemispheric asymmetry of c‐Fos expression in glomeruli and the olfactory tubercle following repeated odor stimulation
Interhemispheric asymmetry of c‐Fos expression in glomeruli and the olfactory tubercle following repeated odor stimulation Open
Odor adaptation allows the olfactory system to regulate sensitivity to different stimulus intensities, which is essential for preventing saturation of the cell‐transducing machinery and maintaining high sensitivity to persistent and repeti…
View article: Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies
Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies Open
In spite of recent developments in mass spectrometry imaging techniques, high-resolution multiplex protein bioimaging techniques are required to unveil the complex inter- and intracellular biomolecular interactions for accurate understandi…
View article: Mass Spectrometry Imaging of Untreated Wet Cell Membranes in Solution Using Single-Layer Graphene
Mass Spectrometry Imaging of Untreated Wet Cell Membranes in Solution Using Single-Layer Graphene Open
We reported on how atomic and molecular secondary ions can be sputtered through single-layer graphene so that untreated wet cell membranes in a solution can be imaged by secondary ion mass spectrometry (SIMS) at subcellular spatial resolut…
View article: Preparation of cellular samples using graphene cover and air-plasma treatment for time-of-flight secondary ion mass spectrometry imaging
Preparation of cellular samples using graphene cover and air-plasma treatment for time-of-flight secondary ion mass spectrometry imaging Open
Cell preparation methods that combine a single-layer graphene cover with air-plasma treatment for improvement of ToF-SIMS imaging.
View article: Single Layer Graphene Assisted Transmission Mode Continuous Wave Laser Desorption for Micrometer Spatial Resolution Atmospheric Pressure Mass Spectrometry Imaging
Single Layer Graphene Assisted Transmission Mode Continuous Wave Laser Desorption for Micrometer Spatial Resolution Atmospheric Pressure Mass Spectrometry Imaging Open
When a fresh tissue slice is placed on a graphene layer substrate and irradiated with a 532 nm continuous wave laser, the transmission mode desorption of biomolecules is found to be greatly enhanced enough to facilitate the mass spectromet…
View article: Nanomaterials and continuous wave laser-based efficient desorption for atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices
Nanomaterials and continuous wave laser-based efficient desorption for atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices Open
Micrometer-resolution mass spectrometric imaging of live hippocampal tissue is achieved with a highly efficient desorption of biomolecules using a 532 nm continuous wave laser and gold nanoparticles or graphene oxide as an energy transport…
View article: Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution
Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution Open
We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is ba…
View article: Future Prospective of Bioimaging with Ion Beams
Future Prospective of Bioimaging with Ion Beams Open
Briefly summarized are our present activities on how to utilize ion beams for bio imaging. We have been trying to extend the analysis scope of bio-SIMS from lipids and metabolites to proteins to make SIMS an omni-molecular imaging tool. To…
View article: SREBP and MDT-15 protect<i>C. elegans</i>from glucose-induced accelerated aging by preventing accumulation of saturated fat
SREBP and MDT-15 protect<i>C. elegans</i>from glucose-induced accelerated aging by preventing accumulation of saturated fat Open
Glucose-rich diets shorten the life spans of various organisms. However, the metabolic processes involved in this phenomenon remain unknown. Here, we show that sterol regulatory element-binding protein (SREBP) and mediator-15 (MDT-15) prev…