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View article: <i>Pseudomonas aeruginosa lasR</i> -deficient mutant contributes to bacterial virulence through enhancing the PhoB-mediated pathway in response to host environment
<i>Pseudomonas aeruginosa lasR</i> -deficient mutant contributes to bacterial virulence through enhancing the PhoB-mediated pathway in response to host environment Open
Pseudomonas aeruginosa is a major opportunistic pathogen that causes lung infections in patients with cystic fibrosis and chronic obstructive pulmonary disease. Loss-of-function mutations in the quorum-sensing regulatory gene lasR commonly…
View article: The fatty acid synthesis gene <i>fabY</i> affects lipopolysaccharide synthesis and colistin susceptibility in <i>Pseudomonas aeruginosa</i>
The fatty acid synthesis gene <i>fabY</i> affects lipopolysaccharide synthesis and colistin susceptibility in <i>Pseudomonas aeruginosa</i> Open
Bacterial metabolism impacts susceptibility to antibiotics. Here, we found that mutation of the Pseudomonas aeruginosa fatty acid response regulator gene pvrA decreases bacterial susceptibility to colistin. The fatty acid synthesis gene fa…
View article: Azithromycin represses evolution of ceftazidime/avibactam resistance by translational repression of <i>rpoS</i> in <i>Pseudomonas aeruginosa</i>
Azithromycin represses evolution of ceftazidime/avibactam resistance by translational repression of <i>rpoS</i> in <i>Pseudomonas aeruginosa</i> Open
Antibiotic combinations can slow down resistance development and/or achieve synergistic therapeutic effects. In this study, we observed that a combined use of ceftazidime-avibactam (CZA) with azithromycin effectively repressed CZA resistan…
View article: Identification of the Pseudomonas aeruginosa AgtR-CspC-RsaL pathway that controls Las quorum sensing in response to metabolic perturbation and Staphylococcus aureus
Identification of the Pseudomonas aeruginosa AgtR-CspC-RsaL pathway that controls Las quorum sensing in response to metabolic perturbation and Staphylococcus aureus Open
Environmental metabolites and metabolic pathways significantly influence bacterial pathogenesis and interspecies competition. We previously discovered that a mutation in the triosephosphate isomerase gene, tpiA , in Pseudomonas aeruginosa …
View article: Roles of rRNA N‐methyladenosine modification in the function of ribosomes
Roles of rRNA N‐methyladenosine modification in the function of ribosomes Open
The N‐methyladenosine (m6A) modification of ribosomal RNA (rRNA) plays critical roles in regulating the function of ribosomes, the essential molecular machines that translate genetic information from mRNA into proteins. Specifically, m6A m…
View article: MvaT binds to the PexsC promoter to repress the type III secretion system in Pseudomonas aeruginosa
MvaT binds to the PexsC promoter to repress the type III secretion system in Pseudomonas aeruginosa Open
Pseudomonas aeruginosa is an opportunistic human pathogen capable of causing a variety of acute and chronic infections. Its type III secretion system (T3SS) plays a critical role in pathogenesis during acute infection. ExsA is a master reg…
View article: Murepavadin induces envelope stress response and enhances the killing efficacies of β-lactam antibiotics by impairing the outer membrane integrity of <i>Pseudomonas aeruginosa</i>
Murepavadin induces envelope stress response and enhances the killing efficacies of β-lactam antibiotics by impairing the outer membrane integrity of <i>Pseudomonas aeruginosa</i> Open
Pseudomonas aeruginosa is a ubiquitous opportunistic pathogen that can cause a variety of acute and chronic infections. The bacterium is highly resistant to numerous antibiotics. Murepavadin is a peptidomimetic antibiotic that blocks the f…
View article: PitA Controls the H2- and H3-T6SSs through PhoB in Pseudomonas aeruginosa
PitA Controls the H2- and H3-T6SSs through PhoB in Pseudomonas aeruginosa Open
Pseudomonas aeruginosa is an opportunistic pathogenic bacterium that causes acute and chronic infections in humans. The type VI secretion systems (T6SSs) have been shown to associate with chronic infections.
View article: MvfR Controls Tolerance to Polymyxin B by Regulating <i>rfaD</i> in Pseudomonas aeruginosa
MvfR Controls Tolerance to Polymyxin B by Regulating <i>rfaD</i> in Pseudomonas aeruginosa Open
Antibiotic resistance imposes a considerable challenge for the treatment of P. aeruginosa infections. Polymyxins are the last-resort antibiotics for the treatment of multidrug-resistant P. aeruginosa infections.
View article: Delivery of spike-RBD by bacterial type three secretion system for SARS-CoV-2 vaccine development
Delivery of spike-RBD by bacterial type three secretion system for SARS-CoV-2 vaccine development Open
COVID-19 pandemic continues to spread throughout the world with an urgent demand for a safe and protective vaccine to effectuate herd protection and control the spread of SARS-CoV-2. Here, we report the development of a bacterial vector CO…
View article: Regulatory and structural mechanisms of PvrA-mediated regulation of the PQS quorum-sensing system and PHA biosynthesis in<i>Pseudomonas aeruginosa</i>
Regulatory and structural mechanisms of PvrA-mediated regulation of the PQS quorum-sensing system and PHA biosynthesis in<i>Pseudomonas aeruginosa</i> Open
Pseudomonas aeruginosa is capable of causing acute and chronic infections in various host tissues, which depends on its abilities to effectively utilize host-derived nutrients and produce protein virulence factors and toxic compounds. Howe…
View article: Pseudomonas aeruginosa Citrate Synthase GltA Influences Antibiotic Tolerance and the Type III Secretion System through the Stringent Response
Pseudomonas aeruginosa Citrate Synthase GltA Influences Antibiotic Tolerance and the Type III Secretion System through the Stringent Response Open
Rising antimicrobial resistance imposes a severe threat to human health. It is urgent to develop novel antimicrobial strategies by understanding bacterial regulation of virulence and antimicrobial resistance determinants.
View article: Pseudomonas aeruginosa Phosphate Transporter PitA (PA4292) Controls Susceptibility to Aminoglycoside Antibiotics by Regulating the Proton Motive Force
Pseudomonas aeruginosa Phosphate Transporter PitA (PA4292) Controls Susceptibility to Aminoglycoside Antibiotics by Regulating the Proton Motive Force Open
Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that causes nosocomial infections in immunocompromised patients. β-lactam and aminoglycoside antibiotics are commonly used in the treatment of P. aeruginosa infections. Pre…
View article: Development of Resistance to Eravacycline by Klebsiella pneumoniae and Collateral Sensitivity-Guided Design of Combination Therapies
Development of Resistance to Eravacycline by Klebsiella pneumoniae and Collateral Sensitivity-Guided Design of Combination Therapies Open
The increasing bacterial antibiotic resistance is a serious threat to global public health, which demands novel antimicrobial medicines and treatment strategies. Eravacycline is a newly approved antibiotic that belongs to the tetracycline …
View article: Design, Synthesis, and Antitumor Evaluation of Novel Mono-Carbonyl Curcumin Analogs in Hepatocellular Carcinoma Cell
Design, Synthesis, and Antitumor Evaluation of Novel Mono-Carbonyl Curcumin Analogs in Hepatocellular Carcinoma Cell Open
Curcumin is a polyphenolic natural product that has promising anticancer properties. However, its clinical utility is limited by its chemical instability and poor metabolic properties. In this paper, a series of new curcumin analogs were s…
View article: Mutation of <i>PA4292</i> in Pseudomonas aeruginosa Increases β-Lactam Resistance through Upregulating Pyocyanin Production
Mutation of <i>PA4292</i> in Pseudomonas aeruginosa Increases β-Lactam Resistance through Upregulating Pyocyanin Production Open
Metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa is increasingly reported worldwide and usually causes infections with high mortality rates. Aztreonam/avibactam is a β-lactam/β-lactamase inhibitor (BLBLI) combination that is unde…
View article: Acetylation of CspC Controls the Las Quorum-Sensing System through Translational Regulation of <i>rsaL</i> in Pseudomonas aeruginosa
Acetylation of CspC Controls the Las Quorum-Sensing System through Translational Regulation of <i>rsaL</i> in Pseudomonas aeruginosa Open
Bacterial infectious diseases impose a severe threat to human health. The ability to orchestrate virulence determinant in response to the host environment is critical for the pathogenesis of bacterial pathogens. Pseudomonas aeruginosa is a…
View article: Dihydrolipoamide Acetyltransferase AceF Influences the Type III Secretion System and Resistance to Oxidative Stresses through RsmY/Z in Pseudomonas aeruginosa
Dihydrolipoamide Acetyltransferase AceF Influences the Type III Secretion System and Resistance to Oxidative Stresses through RsmY/Z in Pseudomonas aeruginosa Open
Carbon metabolism plays an important role in bacterial physiology and pathogenesis. The type III secretion system (T3SS) of Pseudomonas aeruginosa is a virulence factor that contributes to acute infections. It has been demonstrated that ba…
View article: NrtR Mediated Regulation of H1-T6SS in Pseudomonas aeruginosa
NrtR Mediated Regulation of H1-T6SS in Pseudomonas aeruginosa Open
NrtR is a Nudix-related transcriptional regulator and controls the NAD cofactor biosynthesis in bacteria. P. aeruginosa NrtR binds to the intergenic region between nadD2 and pncA to repress the expression of the two operons, therefore cont…
View article: Molecular Characterization of WCK 5222 (Cefepime/Zidebactam)-Resistant Mutants Developed from a Carbapenem-Resistant Pseudomonas aeruginosa Clinical Isolate
Molecular Characterization of WCK 5222 (Cefepime/Zidebactam)-Resistant Mutants Developed from a Carbapenem-Resistant Pseudomonas aeruginosa Clinical Isolate Open
Antibiotic resistance imposes a severe threat on human health. WCK 5222 is a β-lactam/β-lactamase inhibitor combination that is composed of cefepime and zidebactam.
View article: ECF Sigma Factor HxuI Is Critical for <i>In Vivo</i> Fitness of <i>Pseudomonas aeruginosa</i> during Infection
ECF Sigma Factor HxuI Is Critical for <i>In Vivo</i> Fitness of <i>Pseudomonas aeruginosa</i> during Infection Open
P. aeruginosa has a strong ability to adapt to diverse environments, making it capable of causing recurrent and multisite infections in clinics. Understanding host adaptive mechanisms plays an important guiding role in the development of n…
View article: A MexR Mutation Which Confers Aztreonam Resistance to Pseudomonas aeruginosa
A MexR Mutation Which Confers Aztreonam Resistance to Pseudomonas aeruginosa Open
Therapy for Pseudomonas aeruginosa infections is hard due to its high natural and acquirable antibiotic resistance. After colonization in the hosts, P. aeruginosa commonly accumulates genomic mutations which confer them antibiotic resistan…
View article: Acetylation of the CspA family protein CspC controls the type III secretion system through translational regulation of<i>exsA</i>in<i>Pseudomonas aeruginosa</i>
Acetylation of the CspA family protein CspC controls the type III secretion system through translational regulation of<i>exsA</i>in<i>Pseudomonas aeruginosa</i> Open
The ability to fine tune global gene expression in response to host environment is critical for the virulence of pathogenic bacteria. The host temperature is exploited by the bacteria as a cue for triggering virulence gene expression. Howe…
View article: Identification of Novel phoP-phoQ Regulated Genes that Contribute to Polymyxin B Tolerance in Pseudomonas aeruginosa
Identification of Novel phoP-phoQ Regulated Genes that Contribute to Polymyxin B Tolerance in Pseudomonas aeruginosa Open
Polymyxin B and E (colistin) are the last resorts to treat multidrug-resistant Gram-negative pathogens. Pseudomonas aeruginosa is intrinsically resistant to a variety of antibiotics. The PhoP-PhoQ two-component regulatory system contribute…
View article: Mechanisms for Development of Ciprofloxacin Resistance in a Clinical Isolate of Pseudomonas aeruginosa
Mechanisms for Development of Ciprofloxacin Resistance in a Clinical Isolate of Pseudomonas aeruginosa Open
Treatment of infections by Pseudomonas aeruginosa is difficult due to its high intrinsic and acquired antibiotic resistance. Upon colonization in the human hosts, P. aeruginosa accumulates genetic mutations that confer the bacterium antibi…
View article: Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump
Pseudomonas aeruginosa Polynucleotide Phosphorylase Controls Tolerance to Aminoglycoside Antibiotics by Regulating the MexXY Multidrug Efflux Pump Open
Pseudomonas aeruginosa is an opportunistic pathogen that shows high intrinsic resistance to a variety of antibiotics. The MexX-MexY-OprM efflux pump plays an important role in bacterial resistance to aminoglycoside antibiotics.
View article: Research and application of digital virtual maintenance system for steam turbine
Research and application of digital virtual maintenance system for steam turbine Open
After a 430MW machine unit was started with C repair finished, the shafting vibration of it increase rapidly at the phase of on-load. Using the on-line vibration monitoring and fault diagnosis system (TDM system) matched with the unit to c…
View article: Endoribonuclease YbeY Is Essential for RNA Processing and Virulence in Pseudomonas aeruginosa
Endoribonuclease YbeY Is Essential for RNA Processing and Virulence in Pseudomonas aeruginosa Open
The increasing bacterial antibiotic resistance imposes a severe threat to human health. For the development of effective treatment and prevention strategies, it is critical to understand the mechanisms employed by bacteria to grow in the h…