Xiaoting Hua
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View article: Single-cell transcriptomic dynamics exposes hidden survival trajectory under antibiotic treatment
Single-cell transcriptomic dynamics exposes hidden survival trajectory under antibiotic treatment Open
Heterogeneity plays a major role in bacterial resistance to antibiotic treatment while the mechanism at single-cell level is largely unknown. Here, we employed a robust integration of bulk and bacterial single-cell RNA-seq (scRNA-seq) to u…
View article: <i>In vivo</i> development of elevated linezolid resistance mediated by a deletion in ribosomal protein L3 in clinical <i>Enterococcus faecium</i>
<i>In vivo</i> development of elevated linezolid resistance mediated by a deletion in ribosomal protein L3 in clinical <i>Enterococcus faecium</i> Open
Gram-positive bacteria can develop resistance to linezolid through mutations in the 50S large-subunit ribosomal proteins, such as L3. However, this mechanism is rarely reported in clinical enterococcal strains. Here, we describe a pair of …
View article: <i>wspA</i> mutation mediated <i>Pseudomonas aeruginosa</i> rugose small colony variant and its hyper-biofilm
<i>wspA</i> mutation mediated <i>Pseudomonas aeruginosa</i> rugose small colony variant and its hyper-biofilm Open
Pseudomonas aeruginosa is a major multidrug-resistant pathogen whose biofilm formation complicates treatment. P. aeruginosa r ugose s mall c olony v ariants (RSCVs), characterized by enhanced biofilm production and resistance, pose signifi…
View article: An activator regulates the DNA damage response and anti-phage defense networks in <i>Moraxellaceae</i>
An activator regulates the DNA damage response and anti-phage defense networks in <i>Moraxellaceae</i> Open
DNA-damage chemicals, including many antibiotics, often induce prophage induction and phage outbreaks within microbial communities, posing a significant threat to bacterial survival. Moraxellaceae strains are clinically relevant due to the…
View article: Evolution of XDR <i>Pseudomonas aeruginosa</i> ST463 strains with two plasmids harboring multiple antimicrobial resistance genes
Evolution of XDR <i>Pseudomonas aeruginosa</i> ST463 strains with two plasmids harboring multiple antimicrobial resistance genes Open
The extensively drug-resistant (XDR) Pseudomonas aeruginosa ST463 strains, which co-harbor plasmid-associated metallo-β-lactamase (MBL) and bla KPC-2 genes, exhibit significant resistance and virulence, posing great clinical treatment chal…
View article: A phage transcription factor displaces the host σ factor and stabilizes its own σ factor
A phage transcription factor displaces the host σ factor and stabilizes its own σ factor Open
Phages are the most abundant self-replicating entities on earth, and understanding their transcriptional regulation can provide insights into bacterial gene expression mechanisms. The bacterial RNA polymerase core enzyme interacts with var…
View article: Machine learning-based prediction of antimicrobial resistance and identification of AMR-related SNPs in Mycobacterium tuberculosis
Machine learning-based prediction of antimicrobial resistance and identification of AMR-related SNPs in Mycobacterium tuberculosis Open
Background Mycobacterium tuberculosis (MTB) is a human-specific pathogen that primarily infects humans, causing tuberculosis (TB). Antimicrobial resistance (AMR) in MTB presents a formidable challenge to global health. The employment of ma…
View article: Diverse modes of ceftazidime/avibactam resistance acquisition in carbapenem-resistant Klebsiella pneumoniae and Pseudomonas aeruginosa from a Chinese intensive care unit
Diverse modes of ceftazidime/avibactam resistance acquisition in carbapenem-resistant Klebsiella pneumoniae and Pseudomonas aeruginosa from a Chinese intensive care unit Open
View article: Corrigendum to “Diverse Acinetobacter species and Plasmid-Driven spread of carbapenem resistance in pharmaceutical settings in China” [Environ. Int. 198 (2025) 109373]
Corrigendum to “Diverse Acinetobacter species and Plasmid-Driven spread of carbapenem resistance in pharmaceutical settings in China” [Environ. Int. 198 (2025) 109373] Open
View article: Cpx‐mediated amino acid sensing diversifies gastrointestinal colonization of <i>Klebsiella pneumoniae</i>
Cpx‐mediated amino acid sensing diversifies gastrointestinal colonization of <i>Klebsiella pneumoniae</i> Open
Klebsiella pneumoniae is a Gram‐negative opportunistic pathogenic bacterium that occasionally inhabits the human gastrointestinal tracts. Gut‐colonized K. pneumoniae may then metastasize to other organs and tissues, thus causing severe inf…
View article: Diverse Acinetobacter species and Plasmid-Driven spread of carbapenem resistance in pharmaceutical settings in China
Diverse Acinetobacter species and Plasmid-Driven spread of carbapenem resistance in pharmaceutical settings in China Open
Acinetobacter species have emerged as a significant public health concern due to their remarkable capacity to acquire antimicrobial resistance. Environmental reservoirs play a crucial role in spreading antimicrobial resistance genes and po…
View article: Phenotypic and genotypic characterization of clinical carbapenem-resistant <i>Acinetobacter</i> species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China
Phenotypic and genotypic characterization of clinical carbapenem-resistant <i>Acinetobacter</i> species harboring the metallo-beta-lactamases IMP-8 or NDM-1 in China Open
Carbapenem-resistant Acinetobacter spp. pose a significant challenge in clinical settings due to limited treatment options for nosocomial infections. Carbapenem-hydrolyzing class D beta-lactamases are the primary cause for carbapenem resis…
View article: Decadal Evolution of KPC-related plasmids in Pseudomonas aeruginosa high-risk clone ST463 in Zhejiang, China
Decadal Evolution of KPC-related plasmids in Pseudomonas aeruginosa high-risk clone ST463 in Zhejiang, China Open
View article: A regulator controls both DNA damage response and anti-phage defense networks in<i>Moraxellaceae</i>
A regulator controls both DNA damage response and anti-phage defense networks in<i>Moraxellaceae</i> Open
DNA damage chemicals, including many antibiotics, often induce prophage induction and phage outbreaks in microbial communities, a major threat to bacterial survival. Moraxellaceae contains clinically highly relevant strains with outstandin…
View article: Longitudinal genomics reveals carbapenem-resistant Acinetobacter baumannii population changes with emergence of highly resistant ST164 clone
Longitudinal genomics reveals carbapenem-resistant Acinetobacter baumannii population changes with emergence of highly resistant ST164 clone Open
View article: Clinical characteristics and prognosis of bloodstream infections with carbapenem-resistant Gram-negative organisms in patients with hematological malignancies: A multicenter case-control study in China
Clinical characteristics and prognosis of bloodstream infections with carbapenem-resistant Gram-negative organisms in patients with hematological malignancies: A multicenter case-control study in China Open
The antimicrobial resistance of CRO isolates for BSI in HM patients is critical. HM patients with CRO BSI should be treated with appropriate definitive antibiotic therapy based on early clarification of pathology and their antimicrobial su…
View article: Prevalence, antimicrobial resistance, and genomic characterization of Salmonella strains isolated in Hangzhou, China: a two-year study
Prevalence, antimicrobial resistance, and genomic characterization of Salmonella strains isolated in Hangzhou, China: a two-year study Open
View article: Discovery of novel BfmR inhibitors restoring carbapenem susceptibility against carbapenem-resistant <i>Acinetobacter baumannii</i> by structure-based virtual screening and biological evaluation
Discovery of novel BfmR inhibitors restoring carbapenem susceptibility against carbapenem-resistant <i>Acinetobacter baumannii</i> by structure-based virtual screening and biological evaluation Open
The emergence and spread of Acinetobacter baumannii pose a severe threat to public health, highlighting the urgent need for the next generation of therapeutics due to its increasing resistance to existing antibiotics. BfmR, a respon…
View article: Complete genome sequence of <i>Streptococcus intermedius</i> strain XH2169
Complete genome sequence of <i>Streptococcus intermedius</i> strain XH2169 Open
Here, we report the complete genome sequence of Streptococcus intermedius strain XH2169 isolated from a blood sample in China. The genome comprises a single circular chromosome with a length of 1,944,282 bp. It harbored 1,891 coding gene s…
View article: A panel of genotypically and phenotypically diverse clinical <i>Acinetobacter baumannii</i> strains for novel antibiotic development
A panel of genotypically and phenotypically diverse clinical <i>Acinetobacter baumannii</i> strains for novel antibiotic development Open
Acinetobacter baumannii is one of the most important pathogens worldwide. The intrinsic and acquired resistance of A. baumannii , coupled with the slow pace of novel antimicrobial drug development, poses an unprecedented and enormous chall…
View article: Emergence of novel hypervirulent Acinetobacter baumannii strain and herpes simplex type 1 virus in a case of community-acquired pneumonia in China
Emergence of novel hypervirulent Acinetobacter baumannii strain and herpes simplex type 1 virus in a case of community-acquired pneumonia in China Open
Early detection of community-acquired hypervirulent Acinetobacter baumannii strains is recommended to prevent their extensive spread in hospitals.
View article: Genetic characterization of plasmid-borne blaOXA-58 and blaOXA-72 in Acinetobacter pittii in Shaanxi, China
Genetic characterization of plasmid-borne blaOXA-58 and blaOXA-72 in Acinetobacter pittii in Shaanxi, China Open
A. pittii strains with a plasmid carrying blaOXA-58 or blaOXA-72 may serve as an important reservoir of carbapenemase genes. Carbapenemase genes on a single plasmid may facilitate their dissemination and persistence. …
View article: Dissemination of Ceftriaxone-Resistant Salmonella Enteritidis Harboring Plasmids Encoding blaCTX-M-55 or blaCTX-M-14 Gene in China
Dissemination of Ceftriaxone-Resistant Salmonella Enteritidis Harboring Plasmids Encoding blaCTX-M-55 or blaCTX-M-14 Gene in China Open
Salmonella Enteritidis was the primary foodborne pathogen responsible for acute gastroenteritis. The growing ceftriaxone resistance poses a significant threat to public health. Infection with S. Enteritidis has emerged as a major public he…
View article: Precise CRISPR/Cpf1 genome editing system in the Deinococcus radiodurans with superior DNA repair mechanisms
Precise CRISPR/Cpf1 genome editing system in the Deinococcus radiodurans with superior DNA repair mechanisms Open
Deinococcus radiodurans, with its high homologous recombination (HR) efficiency of double-stranded DNA breaks (DSBs), is a model organism for studying genome stability maintenance and an attractive microbe for industrial applications. Here…
View article: Characteristics and phylogenetic distribution of megaplasmids and prediction of a putative chromid in Pseudomonas aeruginosa
Characteristics and phylogenetic distribution of megaplasmids and prediction of a putative chromid in Pseudomonas aeruginosa Open
Research on megaplasmids that contribute to the spread of antimicrobial resistance (AMR) in Pseudomonas aeruginosa strains has grown in recent years due to the now widely used technologies allowing long-read sequencing. Here, we sys…
View article: Changes to an intensive care unit<i>Acinetobacter baumannii</i>population following COVID-19 disruptions and targeted infection prevention interventions
Changes to an intensive care unit<i>Acinetobacter baumannii</i>population following COVID-19 disruptions and targeted infection prevention interventions Open
Carbapenem-resistant Acinetobacter baumannii (CRAB) is a persistent nosocomial pathogen that poses a significant threat to global public health. A three-month cross-sectional observational study was conducted in a 28-bed ICU in Hangzhou, C…
View article: Genome sequencing unveils <i>bla</i> <sub>KPC-2</sub> -harboring plasmids as drivers of enhanced resistance and virulence in nosocomial <i>Klebsiella pneumoniae</i>
Genome sequencing unveils <i>bla</i> <sub>KPC-2</sub> -harboring plasmids as drivers of enhanced resistance and virulence in nosocomial <i>Klebsiella pneumoniae</i> Open
This study is based on a CRKP screening program between patients and ward environments in an intensive care unit, describing the pKPC-2 ( bla KPC-2 -harboring plasmids) population structure and evolutionary characteristics in clinical sett…
View article: <i>bla</i> <sub>KPC-2</sub> overexpression and <i>bla</i> <sub>GES-5</sub> carriage as major imipenem/relebactam resistance mechanisms in <i>Pseudomonas aeruginosa</i> high-risk clones ST463 and ST235, respectively, in China
<i>bla</i> <sub>KPC-2</sub> overexpression and <i>bla</i> <sub>GES-5</sub> carriage as major imipenem/relebactam resistance mechanisms in <i>Pseudomonas aeruginosa</i> high-risk clones ST463 and ST235, respectively, in China Open
Pseudomonas aeruginosa high-risk clones pose severe threats to public health. Here, we characterize the imipenem/relebactam (IR) resistance mechanisms in P. aeruginosa high-risk clones sequence type 235 (ST235) and ST463 in China. Minimum …
View article: IS26 mediated blaCTX-M-65 amplification in Escherichia coli increase the antibiotic resistance to cephalosporin in vivo
IS26 mediated blaCTX-M-65 amplification in Escherichia coli increase the antibiotic resistance to cephalosporin in vivo Open
Our study is an example from the clinic how BLBLIs and β-lactams exposure in vivo possibly promoted the amplification of an IS26-multiple drug resistance (MDR) region. The observation of a copy number variation seen with the blaCTX-M-…
View article: Characterization of two novel VIM-type metallo-β-lactamases, VIM-84 and VIM-85, associated with the spread of IncP-2 megaplasmids in <i>Pseudomonas aeruginosa</i>
Characterization of two novel VIM-type metallo-β-lactamases, VIM-84 and VIM-85, associated with the spread of IncP-2 megaplasmids in <i>Pseudomonas aeruginosa</i> Open
This study aimed to characterize two novel VIM-type metallo-β-lactamases, VIM-84 and VIM-85, and reveal the important role of the IncP-2 type megaplasmids in the spread of antimicrobial resistance (AMR) genes. VIM-84 and VIM-85 were encode…