Xiaoyu Chu
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View article: Recombinant Bacillus subtilis CM65-Patt′ is a potential feed attractant of Megalobrama amblycephala: evaluation based on appetite regulatory system, growth performance, and intestinal functions
Recombinant Bacillus subtilis CM65-Patt′ is a potential feed attractant of Megalobrama amblycephala: evaluation based on appetite regulatory system, growth performance, and intestinal functions Open
This study was conducted to investigate the feed attractant properties of recombinant Bacillus subtilis CM65-P att ′ (secreting P att , a functional peptide screened after enzymatic hydrolysis of the cotton meal) on the appetite regulatory…
View article: Cellular osmoregulation enhances porcine embryo development by restoring zygotic genome activation via metabolic–epigenetic crosstalk
Cellular osmoregulation enhances porcine embryo development by restoring zygotic genome activation via metabolic–epigenetic crosstalk Open
These findings identify that mitochondria in porcine preimplantation embryos act as osmotic stress sensors. Under conditions of extracellular organic osmolyte deficiency and elevated osmolarity, they drive metabolic reprogramming and nucle…
View article: Transcriptomic Profiling of Peripheral Blood Identifies Candidate Genes for Early Pregnancy Diagnosis in Sika Deer
Transcriptomic Profiling of Peripheral Blood Identifies Candidate Genes for Early Pregnancy Diagnosis in Sika Deer Open
With estrus confined to three winter months, early pregnancy detection is essential for reproductive management in farmed sika deer. However, the development of reliable non-invasive early pregnancy detection techniques has been hindered b…
View article: Development and application of a NP-cELISA for the detection of nucleoprotein antibodies of equine influenza virus
Development and application of a NP-cELISA for the detection of nucleoprotein antibodies of equine influenza virus Open
Equine influenza (EI), caused by the equine influenza virus (EIV), is an acute respiratory disease that has become enzootic worldwide, resulting in frequent outbreaks and substantial economic losses within the equine industry. In this stud…
View article: Development of a Broad-Spectrum Antigen-Capture ELISA Using Combined Anti-p26 Polyclonal and Monoclonal Antibodies for Detection of Equine Infectious Anemia Virus
Development of a Broad-Spectrum Antigen-Capture ELISA Using Combined Anti-p26 Polyclonal and Monoclonal Antibodies for Detection of Equine Infectious Anemia Virus Open
Equine Infectious Anemia Virus (EIAV) poses significant diagnostic challenges due to its genetic variability and the limitations of conventional nucleic acid detection methods. This study developed an antigen-capture, enzyme-linked immunos…
View article: LEUTX regulates porcine embryonic genome activation in somatic cell nuclear transfer embryos
LEUTX regulates porcine embryonic genome activation in somatic cell nuclear transfer embryos Open
Emerging evidence highlights the regulatory role of paired-like (PRD-like) homeobox transcription factors (TFs) in embryonic genome activation (EGA). However, the majority of PRD-like genes are lost in rodents, thus prompting an investigat…
View article: The Effectiveness of Four Nicotinamide Adenine Dinucleotide (NAD+) Precursors in Alleviating the High-Glucose-Induced Damage to Hepatocytes in Megalobrama amblycephala: Evidence in NAD+ Homeostasis, Sirt1/3 Activation, Redox Defense, Inflammatory Response, Apoptosis, and Glucose Metabolism
The Effectiveness of Four Nicotinamide Adenine Dinucleotide (NAD+) Precursors in Alleviating the High-Glucose-Induced Damage to Hepatocytes in Megalobrama amblycephala: Evidence in NAD+ Homeostasis, Sirt1/3 Activation, Redox Defense, Inflammatory Response, Apoptosis, and Glucose Metabolism Open
The administration of NAD+ precursors is a potential approach to protect against liver damage and metabolic dysfunction. However, the effectiveness of different NAD+ precursors in alleviating metabolic disorders is still poorly elucidated.…
View article: Dietary supplementation with fermented antarctic krill shell improved the growth performance, digestive and antioxidant capability of Macrobrachium nipponense
Dietary supplementation with fermented antarctic krill shell improved the growth performance, digestive and antioxidant capability of Macrobrachium nipponense Open
To investigate the effects of different levels of fermented antarctic krill shell (FAKS) on the growth performance, hemolymph biochemistry, hepatopancreatic antioxidant capacity, and digestive enzyme activities of Macrobrachium nipponense,…
View article: Development and Application of an iELISA for the Detection of Antibody against Salmonella Abortusequi
Development and Application of an iELISA for the Detection of Antibody against Salmonella Abortusequi Open
Equine abortus salmonellosis is a bacterial disease that causes high abortion rates in susceptible equids and therefore significant economic losses. Although the tube agglutination test (TAT) is a commonly used serological test for S. Abor…
View article: Development and Application of Real-Time PCR Assay for Detection of Salmonella Abortusequi
Development and Application of Real-Time PCR Assay for Detection of Salmonella Abortusequi Open
Salmonella enterica subsp. enterica serovar Abortusequi is a major pathogen in horse and donkey herds, causing abortion in pregnant equids and resulting in enormous economic losses. A rapid and reliable method is urgently needed to detect …
View article: A Discussion on the Practical Teaching Reform of Intelligent Mobile Terminal Technology Course
A Discussion on the Practical Teaching Reform of Intelligent Mobile Terminal Technology Course Open
In this intelligent era, there is a high market demand for professional engineering talents in the computer industry. Based on engineering education accreditation, this paper briefly discusses the practical teaching reform of the intellige…
View article: A Roadmap for Big Model
A Roadmap for Big Model Open
With the rapid development of deep learning, training Big Models (BMs) for multiple downstream tasks becomes a popular paradigm. Researchers have achieved various outcomes in the construction of BMs and the BM application in many fields. A…
View article: Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein
Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein Open
Interferon-mediated host factors myxovirus (Mx) proteins are key features in regulating influenza A virus (IAV) infections. Viral polymerases are essential for viral replication. The Mx1 protein has been known to interact with viral nucleo…
View article: Improving Cadmium Resistance in Escherichia coli Through Continuous Genome Evolution
Improving Cadmium Resistance in Escherichia coli Through Continuous Genome Evolution Open
Cadmium (Cd) is a heavy metal that is extremely toxic to many organisms; however, microbes are highly adaptable to extreme conditions, including heavy metal contamination. Bacteria can evolve in the natural environment, generating resistan…
View article: Presyncodon, a Web Server for Gene Design with the Evolutionary Information of the Expression Hosts
Presyncodon, a Web Server for Gene Design with the Evolutionary Information of the Expression Hosts Open
In the natural host, most of the synonymous codons of a gene have been evolutionarily selected and related to protein expression and function. However, for the design of a new gene, most of the existing codon optimization tools select the …
View article: Identification of bacterial laccase cueO mutation from the metagenome of chemical plant sludge
Identification of bacterial laccase cueO mutation from the metagenome of chemical plant sludge Open
Background The metagenome contains plenty of genetic resources and can be used to search for the novel gene or mutant. Results In this study, the bacterial laccase gene (cueO) with single or multiple mutations was directly cloned based on …
View article: Predicting synonymous codon usage and optimizing the heterologous gene for expression in E. coli
Predicting synonymous codon usage and optimizing the heterologous gene for expression in E. coli Open
Of the 20 common amino acids, 18 are encoded by multiple synonymous codons. These synonymous codons are not redundant; in fact, all of codons contribute substantially to protein expression, structure and function. In this study, the codon …
View article: Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3)
Identification of cadmium resistance and adsorption gene from Escherichia coli BL21 (DE3) Open
Cadmium resistance and adsorption genecapBfromEscherichia coliBL21 (DE3).
View article: Improving the Thermostability of Acidic Pullulanase from Bacillus naganoensis by Rational Design
Improving the Thermostability of Acidic Pullulanase from Bacillus naganoensis by Rational Design Open
Pullulanase (EC 3.2.1.41) plays an important role in the specific hydrolysis of branch points in amylopectin. Enhancing its thermostability is required for its industrial application. In this study, rational protein design was used to impr…
View article: Chaperone gp96 mediates ER-α36 cell membrane expression
Chaperone gp96 mediates ER-α36 cell membrane expression Open
ER (estrogen receptor)-α36, a variant of human ERα, activates non-genomic cell signaling pathways. ER-α36 on the cell membrane plays a role in breast cancer growth and development, and contributes to tamoxifen resistance. However, it is no…
View article: Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy
Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy Open
These results demonstrate that the secretion of heterologous proteins from P. pastoris could be improved by combining changes in multiple protein sequence features.