Xiukun Wan
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View article: Rational Engineering of Patchoulene Synthase from Pogostemon cablin for Enhanced Patchoulene Production
Rational Engineering of Patchoulene Synthase from Pogostemon cablin for Enhanced Patchoulene Production Open
Patchoulene, the characteristic sesquiterpene of patchouli essential oil, is highly valued in the perfume industry for its distinctive woody note and fixative properties. Beyond its olfactory applications, patchoulene has demonstrated prom…
View article: Metabolic Engineering for the Biosynthesis of Pentalenene in the Rapidly Growing Bacterium Vibrio natriegens
Metabolic Engineering for the Biosynthesis of Pentalenene in the Rapidly Growing Bacterium Vibrio natriegens Open
Vibrio natriegens (V. natriegens) is an emerging synthetic biology chassis characterized by rapid growth, and its potential for the synthesis of sesquiterpenes (such as pentalenene) has not been developed. In this study, heterologous penta…
View article: IECata: interpretable bilinear attention network and evidential deep learning improve the catalytic efficiency prediction of enzymes
IECata: interpretable bilinear attention network and evidential deep learning improve the catalytic efficiency prediction of enzymes Open
Enzyme catalytic efficiency (kcat/Km) is a key parameter for identifying high-activity enzymes. Recently, deep learning techniques have demonstrated the potential for fast and accurate kcat/Km prediction. However, three challenges remain: …
View article: IECata: Interpretable bilinear attention network and evidential deep learning improve the catalytic efficiency prediction of enzymes
IECata: Interpretable bilinear attention network and evidential deep learning improve the catalytic efficiency prediction of enzymes Open
Enzyme catalytic efficiency (kcat / Km) is a key parameter for identifying high-activity enzymes. Recently deep learning techniques have demonstrated the potential for fast and accurate kcat / Km…
View article: Genome-wide Identification and Characterization of the Terpenoid Synthase Gene Family in <em>Physcomitrella patens</em>
Genome-wide Identification and Characterization of the Terpenoid Synthase Gene Family in <em>Physcomitrella patens</em> Open
Terpenoids are crucial secondary metabolites found in plants, essential for numerous physiological functions such as defense strategies, signal communication, and adaptation to environmental stresses. The enzymes referred to as Terpene Syn…
View article: MPEK: a multitask deep learning framework based on pretrained language models for enzymatic reaction kinetic parameters prediction
MPEK: a multitask deep learning framework based on pretrained language models for enzymatic reaction kinetic parameters prediction Open
Enzymatic reaction kinetics are central in analyzing enzymatic reaction mechanisms and target-enzyme optimization, and thus in biomanufacturing and other industries. The enzyme turnover number (kcat) and Michaelis constant (Km), key kineti…
View article: The Biosynthesis of the Monoterpene Tricyclene in E. coli through the Appropriate Truncation of Plant Transit Peptides
The Biosynthesis of the Monoterpene Tricyclene in E. coli through the Appropriate Truncation of Plant Transit Peptides Open
Tricyclene, a tricyclic monoterpene naturally occurring in plant essential oils, holds potential for the development of medicinal and fuel applications. In this study, we successfully synthesized tricyclene in E. coli by introducing the he…
View article: MPEK: a multi-task learning based on pre-trained language model for predicting enzymatic reaction kinetic parameters
MPEK: a multi-task learning based on pre-trained language model for predicting enzymatic reaction kinetic parameters Open
Enzymatic reaction kinetics are central in analyzing enzymatic reaction mechanisms and target-enzyme optimization, and thus in biomanufacturing and other industries. The enzyme turnover number ( k cat ) and Michaelis constant ( K m ), key …
View article: Transcriptomic Analysis of the Response of the Toxic Dinoflagellate Prorocentrum lima to Phosphorous Limitation
Transcriptomic Analysis of the Response of the Toxic Dinoflagellate Prorocentrum lima to Phosphorous Limitation Open
Some dinoflagellates cause harmful algal blooms, releasing toxic secondary metabolites, to the detriment of marine ecosystems and human health. Phosphorus (P) is a limiting macronutrient for dinoflagellate growth in the ocean. Previous stu…
View article: Highly Efficient Biosynthesis of γ-Bisabolene with a New Sesquiterpene Synthase AcTPS5 by Dual Cytoplasmic-Peroxisomal Engineering in Saccharomyces cerevisiae
Highly Efficient Biosynthesis of γ-Bisabolene with a New Sesquiterpene Synthase AcTPS5 by Dual Cytoplasmic-Peroxisomal Engineering in Saccharomyces cerevisiae Open
γ-bisabolene is a monocyclic sesquiterpene with various biological activities; it has also been approved as a food additive. Additionally, the hydrogenated form of bisabolene is considered as a potential alternative to D2 diesel. Saccharom…
View article: Systematic Mining and Evaluation of the Sesquiterpene Skeletons as High Energy Aviation Fuel Molecules
Systematic Mining and Evaluation of the Sesquiterpene Skeletons as High Energy Aviation Fuel Molecules Open
Sesquiterpenes have been identified as promising ingredients for aviation fuels due to their high energy density and combustion heat properties. Despite the characterization of numerous sesquiterpene structures, studies testing their perfo…
View article: Design and synthesis of a clickable, photoreactive amino acid <i>p</i>-(4-(but-3-yn-1-yl)benzoyl)-<scp>l</scp>-phenylalanine for peptide photoaffinity labeling
Design and synthesis of a clickable, photoreactive amino acid <i>p</i>-(4-(but-3-yn-1-yl)benzoyl)-<span>l</span>-phenylalanine for peptide photoaffinity labeling Open
The clickable and photoreactive amino acid is prepared in 11 steps from 3-(4-bromophenyl)-1-propanol.
View article: An immobilization-based, loop-mediated isothermal amplification device for nucleic acid detection of SARS-CoV-2 <i>N</i> gene
An immobilization-based, loop-mediated isothermal amplification device for nucleic acid detection of SARS-CoV-2 <i>N</i> gene Open
Confronting the global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), simple, fast and specific non-laboratory SARS-CoV-2 diagnostic tests are urgently required. However, the current nucleic acid assays generally r…
View article: Identification of the sesquiterpene synthase AcTPS1 and high production of (–)-germacrene D in metabolically engineered Saccharomyces cerevisiae
Identification of the sesquiterpene synthase AcTPS1 and high production of (–)-germacrene D in metabolically engineered Saccharomyces cerevisiae Open
View article: A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA
A dynamic and multilocus metabolic regulation strategy using quorum-sensing-controlled bacterial small RNA Open
Metabolic regulation strategies have been developed to redirect metabolic fluxes to production pathways. However, it is difficult to screen out target genes that, when repressed, improve yield without affecting cell growth. Here, we report…
View article: Structural and Functional Characterization of Conotoxins from Conus achatinus Targeting NMDAR
Structural and Functional Characterization of Conotoxins from Conus achatinus Targeting NMDAR Open
Conotoxin-Ac1 and its variant conotoxin-Ac1-O6P, were isolated from the venom duct of Conus achatinus, a fish-hunting cone snail species collected in the Sea of Hainan, China. Conotoxin-Ac1 is linear peptide that contain 15 amino acids. In…
View article: Research Progress in the Biosynthetic Mechanisms of Marine Polyether Toxins
Research Progress in the Biosynthetic Mechanisms of Marine Polyether Toxins Open
Marine polyether toxins, mainly produced by marine dinoflagellates, are novel, complex, and diverse natural products with extensive toxicological and pharmacological effects. Owing to their harmful effects during outbreaks of marine red ti…
View article: <i>Helicobacter pylori</i> inhibits the cleavage of TRAF1 <i>via</i> a CagA-dependent mechanism
<i>Helicobacter pylori</i> inhibits the cleavage of TRAF1 <i>via</i> a CagA-dependent mechanism Open
H. pylori infection significantly inhibits the cleavage of TRAF1 via a CagA-dependent mechanism, which would increase the relative amounts of full-length TRAF1 and exert an antiapoptotic effect on H. pylori-infected ce…