Yen‐Chen Liao
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View article: Spatial top-down proteomics for the functional characterization of human kidney
Spatial top-down proteomics for the functional characterization of human kidney Open
We developed an integrated workflow to directly identify proteoforms and reveal their spatial distributions. Of the 20 differentially abundant proteoforms identified as discriminate between tubules and glomeruli by microPOTS, the vast majo…
View article: Spatial top-down proteomics for the functional characterization of human kidney
Spatial top-down proteomics for the functional characterization of human kidney Open
Background The Human Proteome Project has credibly detected nearly 93% of the roughly 20,000 proteins which are predicted by the human genome. However, the proteome is enigmatic, where alterations in amino acid sequences from polymorphisms…
View article: Spatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform
Spatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform Open
Conventional proteomic approaches measure the averaged signal from mixed cell populations or bulk tissues, leading to the dilution of signals arising from subpopulations of cells that might serve as important biomarkers. Recent development…
View article: Discovery top-down proteomics in symbiotic soybean root nodules
Discovery top-down proteomics in symbiotic soybean root nodules Open
Proteomic methods have been widely used to study proteins in complex biological samples to understand biological molecular mechanisms. Most well-established methods (known as bottom-up proteomics, BUP) employ an enzymatic digestion step to…
View article: Spatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform
Spatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform Open
Conventional proteomic approaches measure the averaged signal from mixed cell populations or bulk tissues, leading to the dilution of signals arising from subpopulations of cells that might serve as important biomarkers. Recent development…
View article: Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis
Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis Open
Although ubiquitously present, information on the function of complex N -glycan posttranslational modification in plants is very limited and is often neglected. In this work, we adopted an enzyme-assisted matrix-assisted laser desorption/i…
View article: Online Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of the SARS-CoV-2 Spike Receptor-Binding Domain
Online Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of the SARS-CoV-2 Spike Receptor-Binding Domain Open
SARS-CoV-2 cellular infection is mediated by the heavily glycosylated spike protein. Recombinant versions of the spike protein and the receptor-binding domain (RBD) are necessary for seropositivity assays and can potentially serve as vacci…