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View article: High-throughput spontaneous Raman imaging of biological specimens with water background reduction
High-throughput spontaneous Raman imaging of biological specimens with water background reduction Open
In multiline-illumination Raman microscopy, background signals from the out-of-focus planes limit the throughput of cell imaging. Here, we improved the throughput of cell imaging by reducing background signals originating from the sample b…
View article: Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging
Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging Open
Raman microscopy is an emerging molecular imaging technology, yet its signal-to-noise ratio (SNR) in measurements of biological specimens is severely limited because of the small cross section of Raman scattering. Here, we present Raman im…
View article: The impact of repeated temperature cycling on cryopreserved human iPSC viability stems from cytochrome redox state changes
The impact of repeated temperature cycling on cryopreserved human iPSC viability stems from cytochrome redox state changes Open
Human induced pluripotent stem cells (hiPSCs) are an attractive cell source for regenerative medicine. For its widespread use as a starting material, a robust storage and distribution system in the frozen state is necessary. For this syste…
View article: On-the-fly Raman microscopy guaranteeing the accuracy of discrimination
On-the-fly Raman microscopy guaranteeing the accuracy of discrimination Open
Accelerating the measurement for discrimination of samples, such as classification of cell phenotype, is crucial when faced with significant time and cost constraints. Spontaneous Raman microscopy offers label-free, rich chemical informati…
View article: Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging
Raman microscopy of cryofixed biological specimens for high-resolution and high-sensitivity chemical imaging Open
Raman microscopy is an emerging molecular imaging technology, yet its signal-to-noise-ratio (SNR) in measurements of biological specimens is severely limited due to the small cross-section of Raman scattering. Here, we present Raman imagin…
View article: Correction for Extrinsic Background in Raman Hyperspectral Images
Correction for Extrinsic Background in Raman Hyperspectral Images Open
Raman hyperspectral microscopy is a valuable tool in biological and biomedical imaging. Because Raman scattering is often weak in comparison to other phenomena, prevalent spectral fluctuations and contaminations have brought advancements i…
View article: Time-deterministic cryo-optical microscopy
Time-deterministic cryo-optical microscopy Open
We developed a technique to rapidly freeze biological systems during observation under an optical microscope, which allows for detailed optical observation at a precise time in the biological dynamics at high spatial resolution and quantif…
View article: Raman imaging of rat nonalcoholic fatty liver tissues reveals distinct biomolecular states
Raman imaging of rat nonalcoholic fatty liver tissues reveals distinct biomolecular states Open
An essential challenge in diagnosing states of nonalcoholic fatty liver disease (NAFLD) is the early prediction of progression from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH) before the disease progresses. Histo…
View article: High-throughput line-illumination Raman microscopy with multislit detection
High-throughput line-illumination Raman microscopy with multislit detection Open
Raman microscopy is an emerging tool for molecular imaging and analysis of living samples. Use of Raman microscopy in life sciences is, however, still limited because of its slow measurement speed for spectral imaging and analysis. We deve…
View article: Differentiability of cell types enhanced by detrending a non-homogeneous pattern in a line-illumination Raman microscope
Differentiability of cell types enhanced by detrending a non-homogeneous pattern in a line-illumination Raman microscope Open
A detrending algorithm for high-speed Raman imaging enhances the detection of cancer cells.
View article: Slit-scanning Raman microscopy: Instrumentation and applications for molecular imaging of cell and tissue
Slit-scanning Raman microscopy: Instrumentation and applications for molecular imaging of cell and tissue Open
In recent years, Raman microscopy has emerged as a molecular imaging tool for cell and tissue analysis. A key reason for this is the development of techniques that significantly increase imaging speed. In this Tutorial, we introduce slit-s…
View article: Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts
Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts Open
Spontaneous Raman spectroscopy, which senses changes in cellular contents of reduced cytochrome c, could be a powerful tool for label-free evaluation of ischemic hearts. However, undetermined is whether it is applicable to evaluation of my…
View article: Fluorescence‐based discrimination of breast cancer cells by direct exposure to 5‐aminolevulinic acid
Fluorescence‐based discrimination of breast cancer cells by direct exposure to 5‐aminolevulinic acid Open
Protoporphyrin IX‐fluorescence measurement is a powerful in situ approach for cancer detection after oral/topical administration of 5‐aminolevulinic acid. However, this approach has not been clinically established for breast cancer, probab…
View article: Hish Resolution Hyperspectral Raman images from Nthy3-1, FTC133 and RO82W-1 cell lines
Hish Resolution Hyperspectral Raman images from Nthy3-1, FTC133 and RO82W-1 cell lines Open
Contains 25 Raw Hyperspectral Raman images measured from Nthyori3-1, FTC133 and RO82W-1 cell lines, after wavenumber calibration. One image data consist of a three dimensional matrix, the first two axis corresponding to the pixel position …
View article: Measuremental Informatics on Single cell Hyperspectral Raman Images for the diagnosis of Follicular Thyroid Carcinoma
Measuremental Informatics on Single cell Hyperspectral Raman Images for the diagnosis of Follicular Thyroid Carcinoma Open
Inter-observer variability and cancer over-diagnosis are emerging clinical problems, and there is a strong necessity to support the standards histological and cytological evaluations by biochemical composition information. Over the past de…
View article: Label-free Molecular Imaging and Analysis by Raman Spectroscopy
Label-free Molecular Imaging and Analysis by Raman Spectroscopy Open
Raman scattering of a cell conveys the intrinsic information inherent to chemical structures of biomolecules. The spectroscopy of Raman scattering, or Raman spectroscopy, allows label-free and quantitative molecular sensing of a biological…
View article: Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy
Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy Open
Raman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability for evaluating myocardial ischaemia is undetermined. Focusing…
View article: Deep-UV biological imaging by lanthanide ion molecular protection
Deep-UV biological imaging by lanthanide ion molecular protection Open
Deep-UV (DUV) light is a sensitive probe for biological molecules such as nucleobases and aromatic amino acids due to specific absorption. However, the use of DUV light for imaging is limited because DUV can destroy or denature target mole…