Yasuteru Urano
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View article: Rapid imaging of pulmonary metastasis from colorectal cancer with a red fluorescence probe targeting puromycin-sensitive aminopeptidase and dipeptidyl peptidase IV
Rapid imaging of pulmonary metastasis from colorectal cancer with a red fluorescence probe targeting puromycin-sensitive aminopeptidase and dipeptidyl peptidase IV Open
Patients with pulmonary metastasis from primary colorectal carcinoma often undergo sublobar pulmonary metastasectomy. To prevent local cancer recurrence, it is important to ensure an appropriate resection margin. For this purpose, rapid an…
View article: EXTH-11. Development of Topically Applied Fluorescent Probes for Intraoperative Diagnosis of Primary Central Nervous System Lymphoma
EXTH-11. Development of Topically Applied Fluorescent Probes for Intraoperative Diagnosis of Primary Central Nervous System Lymphoma Open
BACKGROUND Ring-enhancing lesions observed on gadolinium-enhanced MRI often include primary central nervous system lymphoma (PCNSL), metastatic brain tumors, and glioblastoma. Preoperative differentiation of these lesions remains challengi…
View article: Development of Single‐Molecule Enzyme Activity Assay for Serine Hydrolases Using Activity‐Based Protein Labeling Probes
Development of Single‐Molecule Enzyme Activity Assay for Serine Hydrolases Using Activity‐Based Protein Labeling Probes Open
Single‐molecule enzyme activity assays have proven their potential in elucidating aberrant protein function at the proteoform level. However, the limited number of targetable enzymes is the major drawback of such assays. Here, the developm…
View article: Genetic Code Expansion-Driven <i>in Situ</i> Click Labeling Enables Rapid Imaging-Based Selection of Functional Nanobody–Dye Conjugates
Genetic Code Expansion-Driven <i>in Situ</i> Click Labeling Enables Rapid Imaging-Based Selection of Functional Nanobody–Dye Conjugates Open
Antigen-binding proteins, such as nanobodies, modified with functional small molecules hold great potential for applications including imaging probes, drug conjugates, and localized catalysts. However, traditional chemical labeling methods…
View article: Functional Raman Probes for Detecting Enzyme Activities Based on Aggregation Control
Functional Raman Probes for Detecting Enzyme Activities Based on Aggregation Control Open
Visualizing specific enzyme activities in living systems is important in biomedical research, and Raman imaging probes are particularly suitable for simultaneous multiplexed detection of plural enzyme activities due to their narrow signal …
View article: Rapid imaging of pulmonary metastasis from colorectal cancer with a red fluorescence probe targeting puromycin-sensitive aminopeptidase and dipeptidyl peptidase IV
Rapid imaging of pulmonary metastasis from colorectal cancer with a red fluorescence probe targeting puromycin-sensitive aminopeptidase and dipeptidyl peptidase IV Open
Patients with pulmonary metastasis from primary colorectal carcinoma often undergo sublobar pulmonary metastasectomy. To prevent local cancer recurrence, it is important to ensure an appropriate resection margin. For this purpose, rapid an…
View article: Near-Infrared Coumarin-Hemicyanine Hybrid Dyes Bearing an Intramolecular Nucleophile for Activatable Fluorescence and Raman Imaging
Near-Infrared Coumarin-Hemicyanine Hybrid Dyes Bearing an Intramolecular Nucleophile for Activatable Fluorescence and Raman Imaging Open
Coumarin-hemicyanine (CHC) hybrid fluorophores bearing an intramolecular nucleophile have been reported as ratiometric fluorescent probes based on the equilibrium between the π-conjugated open and cyclic closed forms. Here, we report red-s…
View article: Platform of single-molecule enzyme activity-based liquid biopsy for detection of pancreatic adenocarcinoma at early stages
Platform of single-molecule enzyme activity-based liquid biopsy for detection of pancreatic adenocarcinoma at early stages Open
Liquid biopsy analyzes biomolecules in biofluids to provide critical information for the early detection of diseases and the development of more effective treatment strategies. Among these biomolecules, the functional states of proteins of…
View article: Elucidating the Light-Emitting Species Generated from Aminoluciferin in Firefly/Beetle Bioluminescence
Elucidating the Light-Emitting Species Generated from Aminoluciferin in Firefly/Beetle Bioluminescence Open
Oxyluciferin is the key photon emitter in the firefly/beetle luciferase-catalyzed bioluminescence reaction, and elucidating its chemical form within the active site of luciferase is essential for understanding and modulating emission wavel…
View article: Development of single-molecule enzyme activity assay for serine hydrolases using activity-based protein labeling probes
Development of single-molecule enzyme activity assay for serine hydrolases using activity-based protein labeling probes Open
Single-molecule enzyme activity assays have proven their potential in elucidating aberrant protein function at the proteoform level. However, the limited number of targetable enzymes is the major drawback of such assays. Here, we report th…
View article: Mechanisms of histopathologic vascular damage caused by pancreatic juice leakage: Implications for preventing hemorrhagic complications
Mechanisms of histopathologic vascular damage caused by pancreatic juice leakage: Implications for preventing hemorrhagic complications Open
The activation of pancreatic proteases through mixing with intestinal fluids is a major factor causing histologic damage to the arterial media, leading to aneurysm formation and rupture of the vascular wall. Developing methods for the loca…
View article: Design of Antigen-Targeting Fluorogenic Probes Utilizing Intramolecular Addition Reaction of Protein-Dye Hybrids
Design of Antigen-Targeting Fluorogenic Probes Utilizing Intramolecular Addition Reaction of Protein-Dye Hybrids Open
Fluorogenic probes for antigens are useful for various purposes, including medical diagnostics and imaging, but achieving a rapid, large fluorescence increase is difficult. Here, we report a new class of fluorogenic probes for antigens bas…
View article: Development of an intraoperative margin assessment method for breast cancer using a fluorescence probe targeting α-mannosidase: A multicenter study
Development of an intraoperative margin assessment method for breast cancer using a fluorescence probe targeting α-mannosidase: A multicenter study Open
Background Accurate intraoperative margin assessment is essential to minimize reoperation in breast-conserving surgery (BCS) for breast cancer (BC). This multicenter study aimed to evaluate a novel fluorescent probe targeting α-mannosidase…
View article: Genetic code expansion–driven in situ click labeling enables rapid imaging-based selection of functional nanobody–dye conjugates
Genetic code expansion–driven in situ click labeling enables rapid imaging-based selection of functional nanobody–dye conjugates Open
Antigen-binding proteins, such as nanobodies, modified with functional small molecules hold great potential for applications including imaging probes, drug conjugates and localized catalysts. However, traditional chemical labeling methods …
View article: Low-Background Cancer Imaging With a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety
Low-Background Cancer Imaging With a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety Open
Combinatorial use of an antibody-reporter enzyme conjugate and a fluorescence probe activated by the enzyme is a powerful strategy for fluorescence-guided cancer surgery. However, conventional probes for typical reporter enzymes are insuff…
View article: Development of a near-infrared fluorescent probe for the selective detection of severe hypoxia
Development of a near-infrared fluorescent probe for the selective detection of severe hypoxia Open
We have developed T-azoJSiR640 as a NIR fluorescence probe for severe hypoxia. In contrast to existing NIR probes for hypoxia, the probe was able to selectively visualize severe hypoxia in live cells and in ischemic liver of a mouse model …
View article: New class of fluorogenic probes for antigens based on DARPin-silicon pyronine conjugates
New class of fluorogenic probes for antigens based on DARPin-silicon pyronine conjugates Open
Fluorogenic probes for antigens are useful for various purposes, including medical diagnostics and imaging, but achieving a rapid, large fluorescence increase is difficult. Here, we report a new class of fluorogenic probes for antigens bas…
View article: Non-thermal atmospheric pressure plasma-irradiated cysteine protects cardiac ischemia/reperfusion injury by preserving supersulfides
Non-thermal atmospheric pressure plasma-irradiated cysteine protects cardiac ischemia/reperfusion injury by preserving supersulfides Open
Ischemic heart disease is the main global cause of death in the world. Abnormal sulfide catabolism, especially hydrogen sulfide accumulation, impedes mitochondrial respiration and worsens the prognosis after ischemic insults, but the subst…
View article: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators
Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators Open
Small extracellular vesicles (sEVs) are important intercellular information transmitters in various biological contexts, but their release processes remain poorly understood. Herein, we describe a high-throughput assay platform, C RISPR-as…
View article: Nanoscale dynamics and localization of single endogenous mRNAs in stress granules
Nanoscale dynamics and localization of single endogenous mRNAs in stress granules Open
Stress granules (SGs) are cytoplasmic messenger ribonucleoprotein granules transiently formed in stressed mammalian cells. Although SG components have been well characterized, detailed insights into the molecular behavior inside SGs remain…
View article: Inorganic sulfides prevent osimertinib-induced mitochondrial dysfunction in human iPS cell-derived cardiomyocytes
Inorganic sulfides prevent osimertinib-induced mitochondrial dysfunction in human iPS cell-derived cardiomyocytes Open
Despite the widespread recognition of the global concern regarding the onset of cardiovascular diseases in a significant number of patients following cancer treatment, definitive strategies for prevention and treatment remain elusive. In t…
View article: <scp>D</scp>ipeptidylpeptidase‐4‐targeted activatable fluorescent probes visualize senescent cells
<span>D</span>ipeptidylpeptidase‐4‐targeted activatable fluorescent probes visualize senescent cells Open
Senescent cells promote cancer development and progression through chronic inflammation caused by a senescence‐associated secretory phenotype (SASP). Although various senotherapeutic strategies targeting senescent cells have been developed…
View article: Supersulfide catabolism participates in maladaptive remodeling of cardiac cells
Supersulfide catabolism participates in maladaptive remodeling of cardiac cells Open
The atrophic myocardium resulting from mechanical unloading and nutritional deprivation is considered crucial as maladaptive remodeling directly associated with heart failure, as well as interstitial fibrosis. Conversely, myocardial hypert…
View article: Cyano-Hydrol green derivatives: Expanding the 9-cyanopyronin-based resonance Raman vibrational palette
Cyano-Hydrol green derivatives: Expanding the 9-cyanopyronin-based resonance Raman vibrational palette Open
9-cyanopyronin is a promising scaffold that exploits resonance Raman enhancement to enable sensitive, highly multiplexed biological imaging. Here, we developed cyano-Hydrol Green (CN-HG) derivatives as resonance Raman scaffolds to expand t…
View article: Advancement of fluorescent aminopeptidase probes for rapid cancer detection–current uses and neurosurgical applications
Advancement of fluorescent aminopeptidase probes for rapid cancer detection–current uses and neurosurgical applications Open
Surgical resection is considered for most brain tumors to obtain tissue diagnosis and to eradicate or debulk the tumor. Glioma, the most common primary malignant brain tumor, generally has a poor prognosis despite the multidisciplinary tre…
View article: A general fluorescence off/on strategy for fluorogenic probes: Steric repulsion-induced twisted intramolecular charge transfer (sr-TICT)
A general fluorescence off/on strategy for fluorogenic probes: Steric repulsion-induced twisted intramolecular charge transfer (sr-TICT) Open
Various control strategies are available for building fluorogenic probes to visualize biological events in terms of a fluorescence change. Here, we performed the time-dependent density functional theory (TD-DFT) computational analysis of t…
View article: Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening
Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening Open
Single-molecule enzyme activity-based enzyme profiling (SEAP) is a methodology to globally analyze protein functions in living samples at the single-molecule level. It has been previously applied to detect functional alterations in phospha…
View article: Thioester‐Based Coupled Fluorogenic Assays in Microdevice for the Detection of Single‐Molecule Enzyme Activities of Esterases with Specified Substrate Recognition
Thioester‐Based Coupled Fluorogenic Assays in Microdevice for the Detection of Single‐Molecule Enzyme Activities of Esterases with Specified Substrate Recognition Open
Single‐molecule enzyme activity assay is a platform that enables the analysis of enzyme activities at single proteoform level. The limitation of the targetable enzymes is the major drawback of the assay, but the general assay platform is r…
View article: Characterization of a fluorescence imaging probe that exploits metabolic dependency of ovarian clear cell carcinoma
Characterization of a fluorescence imaging probe that exploits metabolic dependency of ovarian clear cell carcinoma Open
The purpose of this study is to clarify the metabolic dependence of ovarian clear cell carcinoma (CCC) by comparing normal tissues and to examine the applicability of fluorescence imaging probe to exploit these metabolic differences. Enhan…