Yaakov Benenson
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View article: Synthetic gene circuits that selectively target RAS-driven cancers
Synthetic gene circuits that selectively target RAS-driven cancers Open
Therapies targeting mutated RAS, the most frequently mutated oncogene in human cancers, could benefit millions of patients. Recently approved RAS inhibitors represent a breakthrough, but are limited to a specific KRASG12C mutation and pron…
View article: Targeted apoptotic immune modulator for the treatment of metastatic EGFR-positive solid tumors
Targeted apoptotic immune modulator for the treatment of metastatic EGFR-positive solid tumors Open
Aberrant activation and overexpression of the epidermal growth factor receptor (EGFR) occurs in various solid cancers and often correlates with poor outcome. The clinical benefit from EGFR-targeted therapies is usually short-lived, with re…
View article: Peptide Barcodes for miRNA activity assessment in mammalian cells
Peptide Barcodes for miRNA activity assessment in mammalian cells Open
Summary Studies of gene regulation require measurements of mRNA and protein levels of a regulated gene. Recently, parallel reporter assays have been introduced to study the regulation of multiple genes at once. While transcriptional regula…
View article: Synthetic Cytosolic Splicing Enables Programmable mRNA-Encoded Receptors
Synthetic Cytosolic Splicing Enables Programmable mRNA-Encoded Receptors Open
A typical cell therapy product comprises engineered cells that detect disease-related molecular cues in their surrounding or on a surface of a target cell, and activate a response that alleviates disease symptoms or eradicates diseased cel…
View article: Synthetic gene circuits that selectively target RAS-driven cancers
Synthetic gene circuits that selectively target RAS-driven cancers Open
Therapies targeting mutated RAS, the most frequently mutated oncogene in human cancers, could benefit millions of patients. Recently approved RAS inhibitors represent a breakthrough, but are limited to a specific KRASG12C mutation and pron…
View article: Synthetic gene circuits that selectively target RAS-driven cancers
Synthetic gene circuits that selectively target RAS-driven cancers Open
Therapies targeting mutated RAS, the most frequently mutated oncogene in human cancers, could benefit millions of patients. Recently approved RAS inhibitors represent a breakthrough, but are limited to a specific KRASG12C mutation and pron…
View article: Synthetic gene circuits that selectively target RAS-driven cancers
Synthetic gene circuits that selectively target RAS-driven cancers Open
Therapies targeting mutated RAS, the most frequently mutated oncogene in human cancers, could benefit millions of patients. Recently approved RAS inhibitors represent a breakthrough, but are limited to a specific KRAS G12C mutation and pro…
View article: Rational Design and Construction of Multi-Copy Biomanufacturing Islands in Mammalian Cells
Rational Design and Construction of Multi-Copy Biomanufacturing Islands in Mammalian Cells Open
Data underlying the figures in the publication “Rational design and construction of multi-copy biomanufacturing islands in mammalian cells”, published in Nucleic Acids Res., 2022, 50(1), 561-578. DOI:10.1093/nar/gkab1214 Table of contents:…
View article: An AAV Gene Therapy Computes Over Multiple Cellular Inputs to Enable Precise Targeting of Multifocal Hepatocellular Carcinoma in Mice
An AAV Gene Therapy Computes Over Multiple Cellular Inputs to Enable Precise Targeting of Multifocal Hepatocellular Carcinoma in Mice Open
Data underlying the figures in the publication “An AAV gene therapy computes over multiple cellular inputs to enable precise targeting of multifocal hepatocellular carcinoma in mice”, published in Sci. Transl. Med., 2021, 13, eabh4456. DOI…
View article: An AAV Gene Therapy Computes Over Multiple Cellular Inputs to Enable Precise Targeting of Multifocal Hepatocellular Carcinoma in Mice
An AAV Gene Therapy Computes Over Multiple Cellular Inputs to Enable Precise Targeting of Multifocal Hepatocellular Carcinoma in Mice Open
Data underlying the figures in the publication “An AAV gene therapy computes over multiple cellular inputs to enable precise targeting of multifocal hepatocellular carcinoma in mice”, published in Sci. Transl. Med., 2021, 13, eabh4456. DOI…
View article: Rational Design and Construction of Multi-Copy Biomanufacturing Islands in Mammalian Cells
Rational Design and Construction of Multi-Copy Biomanufacturing Islands in Mammalian Cells Open
Data underlying the figures in the publication “Rational design and construction of multi-copy biomanufacturing islands in mammalian cells”, published in Nucleic Acids Res., 2022, 50(1), 561-578. DOI:10.1093/nar/gkab1214 Table of contents:…
View article: A self-assembling peptidic platform to boost the cellular uptake and nuclear delivery of oligonucleotides
A self-assembling peptidic platform to boost the cellular uptake and nuclear delivery of oligonucleotides Open
DNA-loaded peptidic platform entering the cell and delivering its payloads to the nucleus.
View article: Rational design and construction of multi-copy biomanufacturing islands in mammalian cells
Rational design and construction of multi-copy biomanufacturing islands in mammalian cells Open
Cell line development is a critical step in the establishment of a biopharmaceutical manufacturing process. Current protocols rely on random transgene integration and amplification. Due to considerable variability in transgene integration …
View article: Discrete-to-analog signal conversion in human pluripotent stem cells
Discrete-to-analog signal conversion in human pluripotent stem cells Open
During development, state transitions are coordinated through changes in the identity of molecular regulators in a cell state- and dose specific manner. The ability to rationally engineer such functions in human pluripotent stem cells (hPS…
View article: Multiple Alternative Promoters and Alternative Splicing Enable Universal Transcription-Based Logic Computation in Mammalian Cells
Multiple Alternative Promoters and Alternative Splicing Enable Universal Transcription-Based Logic Computation in Mammalian Cells Open
Data underlying the figures in the publication “Multiple Alternative Promoters and Alternative Splicing Enable Universal Transcription-Based Logic Computation in Mammalian Cells”, published in Cell Reports, 2020, 33, 108437. https://doi.or…
View article: Multiple Alternative Promoters and Alternative Splicing Enable Universal Transcription-Based Logic Computation in Mammalian Cells
Multiple Alternative Promoters and Alternative Splicing Enable Universal Transcription-Based Logic Computation in Mammalian Cells Open
Data underlying the figures in the publication “Multiple Alternative Promoters and Alternative Splicing Enable Universal Transcription-Based Logic Computation in Mammalian Cells”, published in Cell Reports, 2020, 33, 108437. https://doi.or…
View article: Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping
Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping Open
Data underlying the figures in the publication “Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping”, published in Nat Commun, 2020, 11, 3551. https://doi.org/10.1038/s41467-020-172…
View article: Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping
Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping Open
Data underlying the figures in the publication “Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping”, published in Nat Commun, 2020, 11, 3551. https://doi.org/10.1038/s41467-020-172…
View article: Precise determination of input-output mapping for multimodal gene circuits using data from transient transfection
Precise determination of input-output mapping for multimodal gene circuits using data from transient transfection Open
The mapping of molecular inputs to their molecular outputs (input/output, I/O mapping) is an important characteristic of gene circuits, both natural and synthetic. Experimental determination of such mappings for synthetic circuits is best …
View article: Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping
Large-scale DNA-based phenotypic recording and deep learning enable highly accurate sequence-function mapping Open
Predicting quantitative effects of gene regulatory elements (GREs) on gene expression is a longstanding challenge in biology. Machine learning models for gene expression prediction may be able to address this challenge, but they require ex…
View article: A self-assembling amphiphilic peptide nanoparticle for the efficient entrapment of DNA cargoes up to 100 nucleotides in length
A self-assembling amphiphilic peptide nanoparticle for the efficient entrapment of DNA cargoes up to 100 nucleotides in length Open
To overcome the low efficiency and cytotoxicity associated with most non-viral DNA delivery systems we developed a purely peptidic self-assembling system that is able to entrap single- and double-stranded DNA of up to 100 nucleotides in le…
View article: Synthetic control systems for high performance gene expression in mammalian cells
Synthetic control systems for high performance gene expression in mammalian cells Open
Tunable induction of gene expression is an essential tool in biology and biotechnology. In spite of that, current induction systems often exhibit unpredictable behavior and performance shortcomings, including high sensitivity to transactiv…
View article: Formal Methods for the Synthesis of Biomolecular Circuits (Dagstuhl Seminar 18082)
Formal Methods for the Synthesis of Biomolecular Circuits (Dagstuhl Seminar 18082) Open
This report documents the program and the outcomes of Dagstuhl Seminar 18082 "Formal Methods for the Synthesis of Biomolecular Circuits". Synthetic biology aims for the rational bottom-up engineering of new biological functionalities. Rece…
View article: Synthetic gene circuits and cellular decision-making in human pluripotent stem cells
Synthetic gene circuits and cellular decision-making in human pluripotent stem cells Open
Precise manipulation of human cell fate is a long-standing goal in biological engineering; it underpins efforts to advance new cellular therapeutics, and to develop disease models for fundamental research. Human pluripotent stem cells (hPS…