Yasuko Manabe
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View article: Non-muscle myosin IIC predominantly expressed in the slow-twitch skeletal muscles impedes age-related muscle weakness
Non-muscle myosin IIC predominantly expressed in the slow-twitch skeletal muscles impedes age-related muscle weakness Open
Skeletal muscle expresses three types of non-muscle myosin (NM) II in addition to skeletal type myosin. While immature myoblasts have been reported to express NMIIA and NMIIB, playing roles in cell morphology, the specific localization and…
View article: R-spondin 3 is a myokine that mediates type I fiber determination during skeletal muscle regeneration
R-spondin 3 is a myokine that mediates type I fiber determination during skeletal muscle regeneration Open
View article: Combined stimuli of elasticity and microgrooves form aligned myotubes that characterize slow twitch muscles
Combined stimuli of elasticity and microgrooves form aligned myotubes that characterize slow twitch muscles Open
View article: <i>Cephalotaxus harringtonia</i> and Their Constituents Harringtonine Alkaloids Inhibit FoxO1 and 3a Activity and Atrophy-Related Gene Expression in C2C12 Myotubes
<i>Cephalotaxus harringtonia</i> and Their Constituents Harringtonine Alkaloids Inhibit FoxO1 and 3a Activity and Atrophy-Related Gene Expression in C2C12 Myotubes Open
The expression of the forkhead box O (FoxO) transcription factor, FoxO, in the skeletal muscle is involved in muscle atrophy caused by disuse, fasting, diabetes, and cachexia. Since inhibition of FoxO activity has been shown to be effectiv…
View article: Combined stimuli of elasticity and microgrooves form aligned myotubes that characterize slow-twitch muscles
Combined stimuli of elasticity and microgrooves form aligned myotubes that characterize slow-twitch muscles Open
Skeletal muscles are classified into slow-twitch muscles composed primarily of type I and IIa fibers with high oxidative metabolism, and fast-twitch muscles composed of type IIx and IIb fibers with high glycolytic metabolism. Fiber-type sh…
View article: Rebastinib inhibits FoxO1 activity and reduces dexamethasone-induced atrophy and its-related gene expression in cultured myotubes
Rebastinib inhibits FoxO1 activity and reduces dexamethasone-induced atrophy and its-related gene expression in cultured myotubes Open
View article: Achieving myoblast engraftment into intact skeletal muscle via extracellular matrix
Achieving myoblast engraftment into intact skeletal muscle via extracellular matrix Open
Cell therapy of skeletal muscles is a promising approach for the prevention of muscular diseases and age-related muscle atrophy. However, cell transplantation to treat muscle atrophy that does not involve disease, such as sarcopenia, is co…
View article: Musashi-2 regulates skeletal muscle fiber type and energy metabolism
Musashi-2 regulates skeletal muscle fiber type and energy metabolism Open
View article: Myokine BDNF highly expressed in Type I fibers inhibits the differentiation of myotubes into Type II fibers
Myokine BDNF highly expressed in Type I fibers inhibits the differentiation of myotubes into Type II fibers Open
Background Myofibers are broadly classified as slow-twitch (Type I) and fast-twitch (Type II) fibers. These two types of myofibers coexist within the same skeletal muscle tissue, determining the contractile and metabolic properties of skel…
View article: Lack of Musashi‐2 induces type <scp>IIa</scp> fiber‐dominated muscle atrophy
Lack of Musashi‐2 induces type <span>IIa</span> fiber‐dominated muscle atrophy Open
Skeletal muscle is a highly plastic tissue, adapting its structure and metabolism in response to diverse conditions such as contractile activity, nutrients, and diseases. Finding a novel master regulator of muscle mass and quality will pro…
View article: Secretion and regenerative function of skeletal muscle
Secretion and regenerative function of skeletal muscle Open
View article: Stable isotope-labeled carnitine reveals its rapid transport into muscle cells and acetylation during contraction
Stable isotope-labeled carnitine reveals its rapid transport into muscle cells and acetylation during contraction Open
Carnitine plays multiple roles in skeletal muscle metabolism, including fatty acid transport and buffering of excess acetyl-CoA in the mitochondria. The skeletal muscle cannot synthesize carnitine; therefore, carnitine must be taken up fro…
View article: PDGF-B secreted from skeletal muscle enhances myoblast proliferation and myotube maturation via activation of the PDGFR signaling cascade
PDGF-B secreted from skeletal muscle enhances myoblast proliferation and myotube maturation via activation of the PDGFR signaling cascade Open
Myokines, secreted factors from skeletal muscle, act locally on muscle cells or satellite cells, which is important in regulating muscle mass and function. Here, we found platelet-derived growth factor subunit B (PDGF-B) is constitutively …
View article: Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate
Establishment of a system evaluating the contractile force of electrically stimulated myotubes from wrinkles formed on elastic substrate Open
View article: R-spondin3 is a myokine that differentiates myoblasts to type I fibres
R-spondin3 is a myokine that differentiates myoblasts to type I fibres Open
View article: Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes
Effect of chronic muscle contraction on expression of contractile and metabolic proteins in mouse primary cultured myotubes Open
Endurance exercise induces skeletal muscle adaptations such as fiber-type switching, mitochondrial biogenesis, angiogenesis, and the enhancement of glucose disposal, all of which ameliorate metabolic dysfunction. Since many factors such as…
View article: Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions
Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions Open
Glucose is a major energy source consumed by proliferating mammalian cells. Therefore, in general, proliferating cells have the preference of high glucose contents in extracellular environment. Here, we showed that high glucose concentrati…
View article: Metabolism
Metabolism Open
Aims] Therapeutic effect of exosomes from skeletal muscles are getting to be focused for the inflammation regulation.The action of acoustic cavitation is reported to facilitate the exosome release from cells.The aim of this study was to in…
View article: Trans-omic Analysis Reveals ROS-Dependent Pentose Phosphate Pathway Activation after High-Frequency Electrical Stimulation in C2C12 Myotubes
Trans-omic Analysis Reveals ROS-Dependent Pentose Phosphate Pathway Activation after High-Frequency Electrical Stimulation in C2C12 Myotubes Open
Skeletal muscle adaptation is mediated by cooperative regulation of metabolism, signal transduction, and gene expression. However, the global regulatory mechanism remains unclear. To address this issue, we performed electrical pulse stimul…
View article: Single-Cell Information Analysis Reveals That Skeletal Muscles Incorporate Cell-to-Cell Variability as Information Not Noise
Single-Cell Information Analysis Reveals That Skeletal Muscles Incorporate Cell-to-Cell Variability as Information Not Noise Open
Cell-to-cell variability in signal transduction in biological systems is often considered noise. However, intercellular variation (i.e., cell-to-cell variability) has the potential to enable individual cells to encode different information…
View article: Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes
Effect of treatment with conditioned media derived from C2C12 myotube on adipogenesis and lipolysis in 3T3-L1 adipocytes Open
Regular exercise is an effective strategy that is used to prevent and treat obesity as well as type 2 diabetes. Exercise-induced myokine secretion is considered a mechanism that coordinates communication between muscles and other organs. I…
View article: Monitoring and mathematical modeling of mitochondrial ATP in myotubes at single‐cell level reveals two distinct population with different kinetics
Monitoring and mathematical modeling of mitochondrial ATP in myotubes at single‐cell level reveals two distinct population with different kinetics Open
Background ATP is the major energy source for myotube contraction, and is quickly produced to compensate ATP consumption and to maintain sufficient ATP level. ATP is consumed mainly in cytoplasm and produced in mitochondria during myotube …
View article: A system for evaluating the contractile force of myotubes, and drug discovery applications
A system for evaluating the contractile force of myotubes, and drug discovery applications Open
Aging and/or disuse of muscles result in losses of muscle mass and function. Muscle atrophy affects not only an individual's quality of life but also healthy life expectancy with increased risks of various diseases. Recent epidemiological …
View article: R3hdml regulates satellite cell proliferation and differentiation
R3hdml regulates satellite cell proliferation and differentiation Open
View article: A new <i>in vitro</i> muscle contraction model and its application for analysis of mTORC1 signaling in combination with contraction and beta-hydroxy-beta-methylbutyrate administration
A new <i>in vitro</i> muscle contraction model and its application for analysis of mTORC1 signaling in combination with contraction and beta-hydroxy-beta-methylbutyrate administration Open
Several food constituents augment exercise-induced muscle strength improvement; however, the detailed mechanism underlying these combined effects is unknown because of the lack of a cultured cell model for evaluating the contraction-induce…
View article: A new <i>in vitro</i> muscle contraction model and its application for analysis of mTORC1 signaling in combination with contraction and beta-hydroxy-beta-methylbutyrate administration
A new <i>in vitro</i> muscle contraction model and its application for analysis of mTORC1 signaling in combination with contraction and beta-hydroxy-beta-methylbutyrate administration Open
Several food constituents augment exercise-induced muscle strength improvement; however, the detailed mechanism underlying these combined effects is unknown because of the lack of a cultured cell model for evaluating the contraction-induce…
View article: Evidence for acute contraction-induced myokine secretion by C2C12 myotubes
Evidence for acute contraction-induced myokine secretion by C2C12 myotubes Open
Skeletal muscle is considered a secretory organ that produces bioactive proteins known as myokines, which are released in response to various stimuli. However, no experimental evidence exists regarding the mechanism by which acute muscle c…
View article: Do Myokines Have Potential as Exercise Mimetics?
Do Myokines Have Potential as Exercise Mimetics? Open
Exercise is generally considered to have health benefits for the body, although its beneficial mechanisms have not been fully elucidated. Recent progressive research suggests that myokines, bioactive substances secreted from skeletal muscl…
View article: Effect of Chronic Muscle Contraction on Endurance Training Associated Protein Expression in Mouse Primary Cultured Myotubes
Effect of Chronic Muscle Contraction on Endurance Training Associated Protein Expression in Mouse Primary Cultured Myotubes Open
Introduction: Endurance training induces a fiber type shift from fast to slow, mitochondria biogenesis, and increased oxidative capacity, which together are known as the skeletal muscle adaptation.To understand how training induces the ske…
View article: Role of satellite cells in skeletal muscle plasticity: Beyond muscle regeneration
Role of satellite cells in skeletal muscle plasticity: Beyond muscle regeneration Open
Satellite cells are resident muscle stem cells located between the basal lamina and the plasma membrane of myofibers. They play crucial roles in muscle growth during the postnatal stage and muscle regeneration following postnatal developme…