Jianren Ye
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View article: Induced resistance to pine wilt disease in Pinus massoniana by the endophytic engineered bacterium Pseudomonas koreensis BM06-P60
Induced resistance to pine wilt disease in Pinus massoniana by the endophytic engineered bacterium Pseudomonas koreensis BM06-P60 Open
Pine wilt disease (PWD), caused by Bursaphelenchus xylophilus, is a globally devastating forest disease. Endophytic bacteria can improve host resistance to diseases by inducing systemic defense responses. We previously constructed an engin…
View article: Identification and characterization of two novel RNA viruses in the pine wood nematode Bursaphelenchus xylophilus
Identification and characterization of two novel RNA viruses in the pine wood nematode Bursaphelenchus xylophilus Open
The pine wood nematode (PWN) Bursaphelenchus xylophilus is a devastating plant pathogen responsible for pine wilt disease. With the development of high-throughput sequencing technology, the virome of important pathogen organisms have been …
View article: Cryopreservation and Maturation Media Optimization for Enhanced Somatic Embryogenesis in Masson Pine (Pinus massoniana)
Cryopreservation and Maturation Media Optimization for Enhanced Somatic Embryogenesis in Masson Pine (Pinus massoniana) Open
Pinus massoniana Lamb. (masson pine) is a critical species for afforestation in southern China but faces severe threats from pine wilt disease (PWD) caused by Bursaphelenchus xylophilus. To accelerate disease-resistant breeding, this study…
View article: Establishment of embryogenic Pinus thunbergii Parl. suspension cultures: growth parameters, dynamic analysis, and plant regenerative capacities
Establishment of embryogenic Pinus thunbergii Parl. suspension cultures: growth parameters, dynamic analysis, and plant regenerative capacities Open
This study presents an approach for selecting appropriate suspension cultures for efficient somatic maturation of P. thunbergii that can also be applied to other conifers. Furthermore, it is possible to commercialize nematode-resistant P. …
View article: Effective Synthesis of mRNA during In Vitro Transcription with Fewer Impurities Produced
Effective Synthesis of mRNA during In Vitro Transcription with Fewer Impurities Produced Open
The remarkable efficacy of COVID-19 vaccines has established mRNA as a highly promising biomedical technology. However, the adequate application of mRNA therapeutics necessitates additional measures to mitigate the inherent immunogenicity,…
View article: Efficient Biosynthesis of Monacolin J through Enzymatic Hydrolysis Using a Recombinant Lovastatin Hydrolase
Efficient Biosynthesis of Monacolin J through Enzymatic Hydrolysis Using a Recombinant Lovastatin Hydrolase Open
Simvastatin is a widely used statin medication that is commonly prescribed to lower cholesterol levels and reduce the risk of cardiovascular events. It is marketed under the brand name Zocor and is known for its effectiveness in treating h…
View article: Unraveling <i>Pinus massoniana</i>’s Defense Mechanisms Against <i>Bursaphelenchus xylophilus</i> Under Aseptic Conditions: A Transcriptomic Analysis
Unraveling <i>Pinus massoniana</i>’s Defense Mechanisms Against <i>Bursaphelenchus xylophilus</i> Under Aseptic Conditions: A Transcriptomic Analysis Open
Pine wilt disease (PWD) is caused by the pine wood nematode (PWN, Bursaphelenchus xylophilus) and significantly impacts pine forest ecosystems globally. This study focuses on Pinus massoniana, an important timber and oleoresin resource in …
View article: Transcriptomic Profiling Reveals That the Differentially Expressed PtNAC9 Transcription Factor Stimulates the Salicylic Acid Pathway to Enhance the Defense Response against Bursaphelenchus xylophilus in Pines
Transcriptomic Profiling Reveals That the Differentially Expressed PtNAC9 Transcription Factor Stimulates the Salicylic Acid Pathway to Enhance the Defense Response against Bursaphelenchus xylophilus in Pines Open
Pinus, a conifer, dominates the world’s forest ecosystems. But it is seriously infected with pine wood nematode (PWN). Transcription factors (TFs) are key regulators in regulating plant resistance. However, the molecular mechanism of TFs r…
View article: Phospho-code of a conserved transcriptional factor underpins fungal virulence
Phospho-code of a conserved transcriptional factor underpins fungal virulence Open
These findings provide a novel insight into the regulatory mechanism of CgMkk1-CgCrzA-CgChs5/6, which enables response of the cell wall inhibitor CFW and facilitates infectious growth for C. gloeosporioides.
View article: Distinct biogeographic patterns for bacteria and fungi in association with <i>Bursaphelenchus xylophilus</i> nematodes and infested pinewood
Distinct biogeographic patterns for bacteria and fungi in association with <i>Bursaphelenchus xylophilus</i> nematodes and infested pinewood Open
Pinewood nematodes (PWN, Bursaphelenchus xylophilus ) are destructive plant parasitic nematodes that cause pine wilt disease (PWD) by attacking the vascular systems of pine trees, resulting in widespread tree mortality. Research has shown …
View article: The Bursaphelenchus xylophilus Effector BxNMP1 Targets PtTLP-L2 to Mediate PtGLU Promoting Parasitism and Virulence in Pinus thunbergii
The Bursaphelenchus xylophilus Effector BxNMP1 Targets PtTLP-L2 to Mediate PtGLU Promoting Parasitism and Virulence in Pinus thunbergii Open
Pinus is an important economic tree species, but pine wilt disease (PWD) seriously threatens the survival of pine trees. PWD caused by Bursaphelenchus xylophilus is a major quarantine disease worldwide that causes significant economic loss…
View article: Genetic Diversity and Population Structure of Bursaphelenchus xylophilus in Guangdong, Guangxi, and Jiangsu Provinces in China
Genetic Diversity and Population Structure of Bursaphelenchus xylophilus in Guangdong, Guangxi, and Jiangsu Provinces in China Open
This study aimed to investigate the genetic structures of pine wood nematodes (PWNs, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle)), in Guangdong (GD), Guangxi (GX), and Jiangsu (JS) Provinces (the major PWN dispersal centers). Fur…
View article: The Growth-Promoting and Colonization of the Pine Endophytic Pseudomonas abietaniphila for Pine Wilt Disease Control
The Growth-Promoting and Colonization of the Pine Endophytic Pseudomonas abietaniphila for Pine Wilt Disease Control Open
In this study, we focused on evaluating the impact of Pseudomonas abietaniphila BHJ04 on the growth of Pinus massoniana seedlings and its biocontrol efficacy against pine wilt disease (PWD). Additionally, the colonization dynamics of P. ab…
View article: Effective Synthesis of High-Integrity mRNA Using In Vitro Transcription
Effective Synthesis of High-Integrity mRNA Using In Vitro Transcription Open
mRNA vaccines are entering a period of rapid development. However, their synthesis is still plagued by challenges related to mRNA impurities and fragments (incomplete mRNA). Most impurities of mRNA products transcribed in vitro are mRNA fr…
View article: Heat stress‐induced oviposition behavioral change correlates with sperm damage in the pine sawyer beetle, <i>Monochamus alternatus</i>
Heat stress‐induced oviposition behavioral change correlates with sperm damage in the pine sawyer beetle, <i>Monochamus alternatus</i> Open
Background Global climate change is causing an increase in extreme high temperatures (EHTs), which subject insects to unprecedented stress. Behavior plasticity in response to EHTs, particularly oviposition behavior, is important for the pe…
View article: Pinus thunbergii Parl. Somatic Plants’ Resistance to Bursaphelenchus xylophilus Depends on Pathogen-Induced Differential Transcriptomic Responses
Pinus thunbergii Parl. Somatic Plants’ Resistance to Bursaphelenchus xylophilus Depends on Pathogen-Induced Differential Transcriptomic Responses Open
Pinus thunbergii Parl. is an economically and medicinally important plant, as well as a world-renowned horticultural species of the Pinus genus. Pine wilt disease is a dangerous condition that affects P. thunbergii. However, understanding …
View article: Optimal culture of Bacillus altitudinis X-10 strain secreting enzyme cocktails for enhancing biomass saccharification of wheat straw
Optimal culture of Bacillus altitudinis X-10 strain secreting enzyme cocktails for enhancing biomass saccharification of wheat straw Open
Developing an efficient and continuous biomass saccharification system provides significant insight into sustainable energy. In the present work, a constant and effective bacterial saccharification system was developed to degrade wheat str…
View article: Effective synthesis of circRNA via a thermostable T7 RNA polymerase variant as the catalyst
Effective synthesis of circRNA via a thermostable T7 RNA polymerase variant as the catalyst Open
Introduction: Circular RNAs (circRNAs) are endogenous noncoding RNAs (ncRNAs) with transcriptional lengths ranging from hundreds to thousands. circRNAs have attracted attention owing to their stable structure and ability to treat complicat…
View article: First evidence that interactions of Heat Shock Protein 20 with reproduction-related proteins aid in fertility recovering from heat stress in Monochamus alternatus
First evidence that interactions of Heat Shock Protein 20 with reproduction-related proteins aid in fertility recovering from heat stress in Monochamus alternatus Open
Insects, as ectotherms, are highly susceptible to temperature increases due to global climate change, with heat tolerance being crucial for their survival. Heat Shock Proteins (HSPs) play a vital role in this tolerance, preventing protein …
View article: Somatic embryogenesis of slash pine (Pinus elliottii Engelm.): initiation, maturation, germination and mycorrhization of regenerated plantlets
Somatic embryogenesis of slash pine (Pinus elliottii Engelm.): initiation, maturation, germination and mycorrhization of regenerated plantlets Open
Slash pine (Pinus elliottii Engelm.), an important economic tree species with a high resin yield, is extensively cultivated in southern China. Somatic embryogenesis (SE) technology could hasten the breeding of P. elliottii through improvin…
View article: Effect of Microbial Inoculants Endowed with Multifarious Plant Growth-Promoting Traits on Grape Growth and Fruit Quality under Organic Fertilization Scenarios
Effect of Microbial Inoculants Endowed with Multifarious Plant Growth-Promoting Traits on Grape Growth and Fruit Quality under Organic Fertilization Scenarios Open
Plant growth-promoting bacteria (PGPB) have growth-promoting and disease-resisting effects and can be used as new types of plant growth promoters. This study was conducted to determine the plant growth-promoting traits of five strains and …
View article: The pine sawyer beetle alters oviposition strategy in response to extreme high temperatures
The pine sawyer beetle alters oviposition strategy in response to extreme high temperatures Open
The frequency and intensity of extreme high temperatures (EHTs) are increasing due to the climate warming. Insects have evolved a range of thermotolerance mechanisms to cope with EHTs, whereas there is limited research available on the alt…
View article: Mechanism of Salt Tolerance and Plant Growth Promotion in Priestia megaterium ZS-3 Revealed by Cellular Metabolism and Whole-Genome Studies
Mechanism of Salt Tolerance and Plant Growth Promotion in Priestia megaterium ZS-3 Revealed by Cellular Metabolism and Whole-Genome Studies Open
Approximately one-third of agricultural land worldwide is affected by salinity, which limits the productivity and sustainability of crop ecosystems. Plant-growth-promoting rhizobacteria (PGPR) are a potential solution to this problem, as P…
View article: Resistant and Susceptible Pinus thunbergii ParL. Show Highly Divergent Patterns of Differentially Expressed Genes during the Process of Infection by Bursaphelenchus xylophilus
Resistant and Susceptible Pinus thunbergii ParL. Show Highly Divergent Patterns of Differentially Expressed Genes during the Process of Infection by Bursaphelenchus xylophilus Open
Pine wilt disease (PWD) is a devastating disease that threatens pine forests worldwide, and breeding resistant pines is an important management strategy used to reduce its impact. A batch of resistant seeds of P. thunbergii was introduced …
View article: Early Diagnosis of Pine Wood Nematode Disease Based on Chlorophyll Fluorescence Parameters and Organic Acids
Early Diagnosis of Pine Wood Nematode Disease Based on Chlorophyll Fluorescence Parameters and Organic Acids Open
Pine wood nematode disease is a destructive disease to the ecological environment and forest resources. The disease is produced by Bursaphelenchus xylophilus, and the onset rate is extremely fast and the pathogenesis of the disease is not …
View article: Toxicity of an Emamectin Benzoate Microemulsion against Bursaphelenchus xylophilus and Its Effect on the Prevention of Pine Wilt Disease
Toxicity of an Emamectin Benzoate Microemulsion against Bursaphelenchus xylophilus and Its Effect on the Prevention of Pine Wilt Disease Open
(1) Pine wilt disease (PWD) is a devastating disease of pine forests caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus. Control of the disease is a worldwide problem due to the impossibility of using chemical nematicides o…
View article: Genetic Diversity and Population Structure of Bursaphelenchus xylophilus in Central China Based on SNP Markers
Genetic Diversity and Population Structure of Bursaphelenchus xylophilus in Central China Based on SNP Markers Open
Hubei, Hunan and Henan Provinces are located in Central China, a region with extensive transport networks and trade. The pine wilt nematode (PWN), Bursaphelenchus xylophilus, the causative agent of pine wilt disease, is spread mainly throu…
View article: The <i>Bursaphelenchus xylophilus</i> candidate effector BxLip‐3 targets the class I chitinases to suppress immunity in pine
The <i>Bursaphelenchus xylophilus</i> candidate effector BxLip‐3 targets the class I chitinases to suppress immunity in pine Open
Lipase is involved in lipid hydrolysis, which is related to nematodes' energy reserves and stress resistance. However, the role of lipases in Bursaphelenchus xylophilus , a notorious plant‐parasitic nematode responsible for severe damage t…