А. І. Yemets
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Features of exosom isolation from tin lacquered mycelium Open
Aim. To apply the proposed methods for obtaining exosomes and select conditions for effective isolation of these nanovesicles from the mycelium of the fungus Ganoderma lucidum (Curtis) P. Karst. To obtain exosomes from the cells of the myc…
Modelling water deficit conditions for investigation of its influence on tomato plants Open
Aim. To model water deficit conditions using polyethylene glycol (PEG) 6000, determine effective concentrations and treatment time of PEG for investigation of the influence of water deficit on the seed germination and morpho-physiological …
Ivermectin’s influence on morpho-physiological parameters of Arabidopsis thaliana L. roots Open
Aim. To study ivermectin’s influence on morphology of roots and root hairs of Arabidopsis thaliana L. seedlings. Methods. In vitro seedlings’ cultivation, microscopy, statistical data analysis. Results. Morphological changes of roots were …
Development of methodological approaches to obtaining of exosome-like nanovesicles from Urtica dioica L. Open
Aim. To develop methodological approaches for the effective isolation of exosome-like nanovesicles from dioecious nettle (Urtica dioica L.) plants, taking into account their purity, quantity and primary characteristics. Methods. The prepar…
Development of the biofabrication method of silver nanoparticles based on Bacillus subtilis and study of their effect on Corynebacterium glutamicum Open
Aim. To develop approaches for the synthesis of silver nanoparticles using Bacillus subtilis (Ehrenberg) Cohn bacteria and to evaluate their toxicity against non-pathogenic soil microflora, particularly the bacterium Corynebacterium glutam…
Yeasts as Biofertilizers and Biocontrol Agents: Mechanisms and Applications Open
Yeasts, a large and diverse group of microorganisms, are gaining increasing interest from both the scientific community and industry. Yeasts’ natural association with plants has endowed them with a broad repertoire of mechanisms that facil…
Development of biosensor technologies for the determination of genetically modified organisms Open
In the nearly three decades since genetically modified organisms (GMOs) were first commercialized, genetically modified (GM) crops have gained an advantage over their conventional counterparts. The main traits introduced into commercialize…
Functional interaction of melatonin with gasotransmitters and ROS in plant adaptation to abiotic stresses Open
Melatonin is considered a multifunctional stress metabolite and a novel plant hormone affecting seed germination, root architecture, circadian rhythms, leaf senescence, and fruit ripening. Melatonin functions related to plant adaptation to…
Effect of Fullerene C60 on Tomato Plants Open
Background. Fullerenes, one of the allotropic forms of carbon, are the focus of intensive research in nanobiotechnology. Due to their unique physicochemical properties, there is growing interest in using them to enhance plant productivity…
Investigation of phytotoxic effects of fullerene С60 with the use of Allium-test Open
Aim. To investigate the cytogenetic effects of water-soluble fullerene C60 with the use of Allium test. To study the influence of various concentrations of fullerene С60 on the morpho-physiological parameters of A. cepa. Methods. The influ…
Comparative analysis of Brassica carinata and fungal industrial lipases used for biodiesel production Open
Aim. To identify the genes of endogenous TAG-lipases in the genome of Abyssinian mustard, or carinata (Brassica carinata), and to analyze the similarity of their amino acid sequences with the industrial lipases of fungal origin. Methods. G…
Design of potential Ilp markers for Hordeum vulgare and H. marinum Atg2, Atg7, Atg8 genes Open
Aim. Design of potential intron length polymorphism markers for ATG2, ATG7, ATG8 genes. Methods. Use of classical bioinformatics methods to search for loci encoding ATG2, ATG7, ATG8 in the Hordeum marinum genome based on homology with cult…
Features of the isolation of yeast from the root microbiome of winter wheat (Triticum aestivum L.) Open
Aim. Development of approaches for isolating yeast symbionts from the rhizosphere of various varieties of winter wheat for their further taxonomic identification, as well as studying the influence of yeast species on the growth and develop…
Investigation of the influence of ivermectin on potato plants in vitro Open
Aim. Investigation of the influence of ivermectin dissolved in different organic solvents (DMSO and ethanol) on morpho-physiological parameters of potato plants (Solanum lycopersicum) in vitro. Methods. Potato plants of cv. Vernisage were …
Self-Organized SERS Substrates with Efficient Analyte Enrichment in the Hot Spots Open
One of the requirements of an efficient surface-enhanced Raman spectroscopy (SERS) substrate is a developed surface morphology with a high density of "hot spots", nm-scale spacings between plasmonic nanoparticles. Of particular interest ar…
The Use of PGPB-based Bioformulations to Control Bacterial Diseases of Vegetable Crops in Ukraine Open
Ukraine is one of the largest producers of vegetable products in the world. The sustainable development of this industry in Ukraine is vital for the food security of many countries worldwide. Recently, farmers in Ukraine are facing the cha…
View article: Peculiarities of physical therapy for persistent bronchial asthma in order to preserve physical capacity and improve the psycho-emotional state of patients
Peculiarities of physical therapy for persistent bronchial asthma in order to preserve physical capacity and improve the psycho-emotional state of patients Open
Aim: The aim of the study is to experimentally determine the effectiveness of the author’s technique of respiratory gymnastics and psychocorrective exercises for use in the polyclinic period during the rehabilitation of patients with persi…
Ivermectin affects Arabidopsis thaliana microtubules through predicted binding site of β-tubulin Open
The ivermectin is a potent nematocide and insecticide, which has low toxicity for humans and domestic animals, but due to low biotransformation, it can be dangerous for non-target organisms. The recent determination of ivermectin absorptio…
Biotechnological approaches for enhancing the resistance of tomato plants to phytopathogenic bacteria Open
Bacterial diseases of vegetable crops cause significant losses of yield and substantially decrease food quality. For sustainable development of agriculture, it is highly important to use the most effective strategies for the protection of …
Facile SERS substrates from Ag nanostructures chemically synthesized on glass surfaces Open
A quick one-step fabrication of efficient SERS substrates by a modified approach based on a silver-mirror reaction (using Tollens’ reagent) is reported. Commercially available microscope slides or cover glass (coverslips) were used as-rece…
Investigation of the influence of avermectin-containing preparations on the resistance of tomato lines to fusarium blight in vitro Open
Aim. Studying of the influence of avermectin-containing polyfunctional preparations Avercom and Avercom Nova on the enhancement of the resistance to fusarium wilt of tomato plants in vitro. Methods. Tomato plants were cultivated in vitro f…
ELUCIDATION OF CELLULAR MECHANISMS OF AUTOPHAGY INVOLVEMENT IN PLANT ADAPTATION TO MICROGRAVITY CONDITIONS Open
It was shown that clinostating conditions induce autophagy without increasing of programmed cell death (PCD) index in the epidermal cells of the root apex of A. thaliana seedlings. After the phase of activation of autophagy, its regulatory…
The role of nitric oxide and hydrogen sulfide in regulation of redox homeostasis at extreme temperatures in plants Open
Nitric oxide and hydrogen sulfide, as important signaling molecules (gasotransmitters), are involved in many functions of plant organism, including adaptation to stress factors of various natures. As redox-active molecules, NO and H 2 S ar…
Biotechnological Approaches to Increase the Bacterial and Fungal Disease Resistance in Potato Open
Potato (Solanum tuberosum) is a valuable cultivated vegetable with high nutritional qualities containing a variety of essential ingredients such as starch, vitamins and essential minerals. However cultivating potatoes is significantly comp…
Microgravity Induces Interplay Between Atg8, Tua and Tub Gene Expression in Arabidopsis Thaliana Open
Autophagy plays a direct or indirect roles in plant growth and development, pathogen invasion and immunity. It is involved in the control of cell homeostasis and modulates plant response and adaptation to various abiotic stress stimuli. Th…
Wheat Genes Associated with Different Types of Resistance against Stem Rust (Puccinia graminis Pers.) Open
Stem rust is one wheat’s most dangerous fungal diseases. Yield losses caused by stem rust have been significant enough to cause famine in the past. Some races of stem rust are considered to be a threat to food security even nowadays. Resis…
View article: Genome-wide identification and analysis of the cytokinin oxidase/dehydrogenase (ckx) gene family in finger millet (Eleusine coracana)
Genome-wide identification and analysis of the cytokinin oxidase/dehydrogenase (ckx) gene family in finger millet (Eleusine coracana) Open
Cytokinin dehydrogenase/oxidase (CKX) enzymes play a key role in regulating cytokinin (CK) levels in plants by degrading the excess of this phytohormone. CKX genes have proven an attractive target for genetic engineering, as their silencin…