G Yeo
YOU?
Author Swipe
View article: KIF5A binds RNA to orchestrate synaptic mRNA localization and stress granules in ALS
KIF5A binds RNA to orchestrate synaptic mRNA localization and stress granules in ALS Open
Neuronal health depends on the precise transport and local translation of mRNAs to maintain synaptic function across highly polarized cellular architecture. While kinesin motor proteins are known to mediate mRNA transport, the specificity …
View article: Dosage-sensitive <i>RBFOX2</i> autoregulation promotes cardiomyocyte differentiation by maturing the transcriptome
Dosage-sensitive <i>RBFOX2</i> autoregulation promotes cardiomyocyte differentiation by maturing the transcriptome Open
SUMMARY Haploinsufficiency of the RNA splicing regulator, RBFOX2 , is linked to congenital heart disease (CHD), yet its pathogenic mechanisms remain unclear. Here, we demonstrate that RBFOX2 is essential for progressing cardiomyocyte (CM) …
View article: The IFIT2–IFIT3 antiviral complex targets short 5’ untranslated regions on viral mRNAs for translation inhibition
The IFIT2–IFIT3 antiviral complex targets short 5’ untranslated regions on viral mRNAs for translation inhibition Open
Recognition of foreign RNA is critical for the innate immune response to viruses. Interferon (IFN)-induced proteins with tetratricopeptide repeats (IFIT) 2 and 3 are highly upregulated following viral infection, but mechanistic insight int…
View article: Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs
Enhancing RNA base editing on mammalian transcripts with small nuclear RNAs Open
Endogenous uridine-rich small nuclear RNAs (U snRNAs) form RNA–protein complexes to process eukaryotic pre-mRNA into mRNA. Previous studies have demonstrated programmable U snRNA guide-targeted exon inclusion and exclusion. Here we investi…
View article: Aberrant host mRNA partitioning in Ebola virus condensates driven by RNA folding perpetuates species-dependent interferon response
Aberrant host mRNA partitioning in Ebola virus condensates driven by RNA folding perpetuates species-dependent interferon response Open
RNA viruses form membraneless condensates in host cells to drive replication, but whether these compartments also regulate host RNAs remains unclear. Using MERFISH-based subcellular transcriptomics, we quantified cellular mRNA recruitment …
View article: Biotin-Labelling of Immunoprecipitated RNA (v2) v1
Biotin-Labelling of Immunoprecipitated RNA (v2) v1 Open
RNA binding proteins (RBPs) regulate a diverse array of RNA processing steps by binding RNAs through sequence and structural elements. The development of crosslinking and immunoprecipitation (CLIP) enabled identification of RBP targets tra…
View article: Investigational eIF2B activator DNL343 modulates the integrated stress response in preclinical models of TDP-43 pathology and individuals with ALS in a randomized clinical trial
Investigational eIF2B activator DNL343 modulates the integrated stress response in preclinical models of TDP-43 pathology and individuals with ALS in a randomized clinical trial Open
Neuronal TDP-43 aggregates are a hallmark ALS pathology. The integrated stress response (ISR) occurs downstream of TDP-43 pathology and may promote neurodegeneration. Here we demonstrate that a CNS penetrant small molecule eIF2B activator …
View article: Rational Generation of Monoclonal Antibodies and Intrabodies Selective for Pathogenic TDP-43
Rational Generation of Monoclonal Antibodies and Intrabodies Selective for Pathogenic TDP-43 Open
TAR DNA-binding protein 43 (TDP-43), encoded by the TARDBP gene, is a ribonucleoprotein associated with the pathogenesis of amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and Alzheimer’s disease (AD). Under physiologic…
View article: Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONO
Structural and mechanistic analysis of covalent ligands targeting the RNA-binding protein NONO Open
Summary RNA-binding proteins (RBPs) play important roles in mRNA transcription, processing, and translation. Chemical tools are lacking for RBPs, which has hindered efforts to perturb and understand RBP function in cells. We recently repor…
View article: Human CCR4 deadenylase homolog Angel1 is a non-stop mRNA decay factor
Human CCR4 deadenylase homolog Angel1 is a non-stop mRNA decay factor Open
Translation elongation stalls trigger mRNA decay and degradation of the nascent polypeptide via translation-dependent quality control pathways. One such pathway, non-stop mRNA decay (NSD), targets aberrant mRNAs that lack stop codons, for …
View article: Evaluation of novel computational methods to identify RNA-binding protein footprints from structural data
Evaluation of novel computational methods to identify RNA-binding protein footprints from structural data Open
RNA-binding proteins (RBP) play diverse roles in mRNA processing and function. However, from thousands of RBPs encoded in the human genome, a detailed molecular understanding of their interactions with RNA is available only for a small fra…
View article: EvidenceBench: A Benchmark for Extracting Evidence from Biomedical Papers
EvidenceBench: A Benchmark for Extracting Evidence from Biomedical Papers Open
We study the task of automatically finding evidence relevant to hypotheses in biomedical papers. Finding relevant evidence is an important step when researchers investigate scientific hypotheses. We introduce EvidenceBench to measure model…
View article: Stratification of enterochromaffin cells by single-cell expression analysis
Stratification of enterochromaffin cells by single-cell expression analysis Open
Dynamic interactions between gut mucosal cells and the external environment are essential to maintain gut homeostasis. Enterochromaffin (EC) cells transduce both chemical and mechanical signals and produce 5-hydroxytryptamine to mediate di…
View article: Integrated multi-omic characterizations of the synapse reveal RNA processing factors and ubiquitin ligases associated with neurodevelopmental disorders
Integrated multi-omic characterizations of the synapse reveal RNA processing factors and ubiquitin ligases associated with neurodevelopmental disorders Open
The molecular composition of the excitatory synapse is incompletely defined due to its dynamic nature across developmental stages and neuronal populations. To address this gap, we apply proteomic mass spectrometry to characterize the synap…
View article: Stratification of enterochromaffin cells by single-cell expression analysis
Stratification of enterochromaffin cells by single-cell expression analysis Open
Dynamic interactions between gut mucosal cells and the external environment are essential to maintain gut homeostasis. Enterochromaffin (EC) cells transduce both chemical and mechanical signals and produce 5-hydroxytryptamine (5-HT) to med…
View article: Short 5’ UTRs serve as a marker for viral mRNA translation inhibition by the IFIT2-IFIT3 antiviral complex
Short 5’ UTRs serve as a marker for viral mRNA translation inhibition by the IFIT2-IFIT3 antiviral complex Open
Recognition of “non-self” nucleic acids, including cytoplasmic dsDNA, dsRNA, or mRNAs lacking proper 5’ cap structures, is critical for the innate immune response to viruses. Here, we demonstrate that short 5’ untranslated regions (UTRs), …
View article: Phenylpyrazoles as Inhibitors of the m<sup>6</sup>A RNA-Binding Protein YTHDF2
Phenylpyrazoles as Inhibitors of the m<sup>6</sup>A RNA-Binding Protein YTHDF2 Open
The N6-methyladenosine (m6A) modification, which is the most common RNA modification in eukaryotes, is regulated by the "writer" methyltransferases, the "reader" m6A binding proteins, and the "eraser" demethylases. m6A plays a multifunctio…
View article: Tissue-resident memory CD8 T cell diversity is spatiotemporally imprinted
Tissue-resident memory CD8 T cell diversity is spatiotemporally imprinted Open
Tissue-resident memory CD8 T (T RM ) cells provide protection from infection at barrier sites. In the small intestine, T RM cells are found in at least two distinct subpopulations: one with higher expression of effector molecules and anoth…
View article: LARP6 regulates the mRNA translation of fibrogenic genes in liver fibrosis
LARP6 regulates the mRNA translation of fibrogenic genes in liver fibrosis Open
Summary Metabolic syndrome and excessive alcohol consumption result in liver injury and fibrosis, which is characterized by increased collagen production by activated Hepatic Stellate Cells (HSCs). LARP6, an RNA-binding protein, was shown …
View article: Stress Granules Underlie Acute Myeloid Leukemia Stem Cell Survival and Stress Adaptation
Stress Granules Underlie Acute Myeloid Leukemia Stem Cell Survival and Stress Adaptation Open
The link between cancer maintenance and an ability to sustain continued growth through stresses conferred by the cancer state itself is growing. However, there are significant gaps in our understanding of how this stress is managed, partic…
View article: Localized molecular chaperone synthesis maintains neuronal dendrite proteostasis
Localized molecular chaperone synthesis maintains neuronal dendrite proteostasis Open
Proteostasis is maintained through regulated protein synthesis and degradation and chaperone-assisted protein folding. However, this is challenging in neuronal projections because of their polarized morphology and constant synaptic proteom…
View article: Rational design yields RNA-binding zinc finger domains with altered sequence specificity
Rational design yields RNA-binding zinc finger domains with altered sequence specificity Open
Targeting and manipulating endogenous RNAs in a sequence-specific manner is essential for both understanding RNA biology and developing RNA-targeting therapeutics. RNA-binding zinc fingers (ZnFs) are excellent candidates as designer protei…
View article: Supra- and sub-threshold intracellular-like recording of 2D and 3D neuronal networks using nanopillar electrode arrays
Supra- and sub-threshold intracellular-like recording of 2D and 3D neuronal networks using nanopillar electrode arrays Open
The brain integrates activity across networks of interconnected neurons to generate behavioral outputs. Several physiological and imaging-based approaches have been previously used to monitor responses of individual neurons. While these te…
View article: Strategies for programmable manipulation of alternative splicing
Strategies for programmable manipulation of alternative splicing Open
Alternative splicing (AS) plays a pivotal role in protein diversity and mRNA maturation. Programmable control of targeted AS events is of longstanding interest in RNA biology, promising correction of dysregulated splicing in disease and di…