Yeonjin Ko
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View article: Targeted protein degradation by KLHDC2 ligands identified by high-throughput screening
Targeted protein degradation by KLHDC2 ligands identified by high-throughput screening Open
Proteolysis-targeting chimeras (PROTACs) enable the selective and sub-stoichiometric elimination of pathological proteins, yet only two E3 ligases are routinely used for this purpose. Here, we expand the repertoire of PROTAC-compatible E3 …
View article: Targeted protein degradation by KLHDC2 ligands identified by high-throughput screening
Targeted protein degradation by KLHDC2 ligands identified by high-throughput screening Open
Proteolysis-targeting chimeras (PROTACs) enable the selective and sub-stoichiometric elimination of pathological proteins, yet only two E3 ligases are routinely used for this purpose. Here, we expand the repertoire of PROTAC-compatible E3 …
View article: Targeted Protein Degradation by KLHDC2 Ligands Identified by High Throughput Screening
Targeted Protein Degradation by KLHDC2 Ligands Identified by High Throughput Screening Open
Proteolysis targeting chimeras (PROTACs) enable the selective and sub-stoichiometric elimination of pathological proteins, yet only two E3 ligases are routinely used for this purpose. Here, we expand the repertoire of PROTAC compatible E3 …
View article: Targeted Protein Degradation by KLHDC2 Ligands Identified by High Throughput Screening
Targeted Protein Degradation by KLHDC2 Ligands Identified by High Throughput Screening Open
Proteolysis targeting chimeras (PROTACs) enable the selective and sub-stoichiometric elimination of pathological proteins, yet only two E3 ligases are routinely used for this purpose. Here, we expand the repertoire of PROTAC compatible E3 …
View article: Succinylation of a KEAP1 sensor lysine promotes NRF2 activation
Succinylation of a KEAP1 sensor lysine promotes NRF2 activation Open
Summary Crosstalk between metabolism and stress-responsive signaling is essential to maintaining cellular homeostasis. One way this crosstalk is achieved is through the covalent modification of proteins by endogenous, reactive metabolites …
View article: S-lactoyl modification of KEAP1 by a reactive glycolytic metabolite activates NRF2 signaling
S-lactoyl modification of KEAP1 by a reactive glycolytic metabolite activates NRF2 signaling Open
KEAP1 (Kelch-like ECH-associated protein), a cytoplasmic repressor of the oxidative stress responsive transcription factor Nuclear factor erythroid 2-related factor 2 (NRF2), senses the presence of electrophilic agents by modification of i…
View article: An mTOR-independent Macroautophagy Activator Ameliorates Tauopathy and Prionopathy Neurodegeneration Phenotypes
An mTOR-independent Macroautophagy Activator Ameliorates Tauopathy and Prionopathy Neurodegeneration Phenotypes Open
Summary Autophagy-lysosomal impairment is an early and prominent feature of neurodegeneration. Autophagy activation reduces protein aggregates and lipid level abnormalities. We performed a high-content imaging-based screen assessing 940,00…
View article: Dioxane Bridge Formation during the Biosynthesis of Spectinomycin Involves a Twitch Radical<i>S</i>-Adenosyl Methionine Dehydrogenase That May Have Evolved from an Epimerase
Dioxane Bridge Formation during the Biosynthesis of Spectinomycin Involves a Twitch Radical<i>S</i>-Adenosyl Methionine Dehydrogenase That May Have Evolved from an Epimerase Open
Spectinomycin is a dioxane-bridged, tricyclic aminoglycoside produced by Streptomyces spectabilis ATCC 27741. While the spe biosynthetic gene cluster for spectinomycin has been reported, the chemistry underlying construction of the dioxane…
View article: A short ORF-encoded transcriptional regulator
A short ORF-encoded transcriptional regulator Open
Significance The small size of short ORF-encoded peptides (SEPs) has made it difficult to identify their cellular binding partners using standard methods. Here, we show that the photo-crosslinking amino acid AbK can be incorporated into ov…
View article: Characterization of the coformycin biosynthetic gene cluster in <i>Streptomyces kaniharaensis</i>
Characterization of the coformycin biosynthetic gene cluster in <i>Streptomyces kaniharaensis</i> Open
Significance Coformycin and pentostatin are potent nucleoside inhibitors of adenosine deaminase with anticancer activity due to their structurally unique 1, 3-diazepine nucleobases. Herein, the biosynthetic pathway of coformycin is reconst…
View article: Toward a Synthetic Yeast Endosymbiont with a Minimal Genome
Toward a Synthetic Yeast Endosymbiont with a Minimal Genome Open
Based on the endosymbiotic theory, one of the key events that occurred during mitochondrial evolution was an extensive loss of nonessential genes from the protomitochondrial endosymbiont genome and transfer of some of the essential endosym…
View article: Identification of the Formycin A Biosynthetic Gene Cluster from <i>Streptomyces kaniharaensis</i> Illustrates the Interplay between Biological Pyrazolopyrimidine Formation and <i>de Novo</i> Purine Biosynthesis
Identification of the Formycin A Biosynthetic Gene Cluster from <i>Streptomyces kaniharaensis</i> Illustrates the Interplay between Biological Pyrazolopyrimidine Formation and <i>de Novo</i> Purine Biosynthesis Open
Formycin A is a potent purine nucleoside antibiotic with a C-glycosidic linkage between the ribosyl moiety and the pyrazolopyrimidine base. Herein, a cosmid is identified from the Streptomyces kaniharaensis genome library that contains the…
View article: Engineering yeast endosymbionts as a step toward the evolution of mitochondria
Engineering yeast endosymbionts as a step toward the evolution of mitochondria Open
Significance Endosymbiotic theory suggests that mitochondria evolved from free-living prokaryotes which entered the host cell and were retained as endosymbionts. Here, we model this earliest stage of the endosymbiotic theory of mitochondri…
View article: Mechanistic Implications of the Deamination of TDP-4-amino-4-deoxy-<scp>d</scp>-fucose Catalyzed by the Radical SAM Enzyme DesII
Mechanistic Implications of the Deamination of TDP-4-amino-4-deoxy-<span>d</span>-fucose Catalyzed by the Radical SAM Enzyme DesII Open
DesII is a radical SAM lyase that catalyzes a deamination reaction during the biosynthesis of desosamine in Streptomyces venezuelae. Competing mechanistic hypotheses for this radical-mediated reaction are differentiated according to whethe…
View article: Identification and Characterization of Enzymes Catalyzing Pyrazolopyrimidine Formation in the Biosynthesis of Formycin A
Identification and Characterization of Enzymes Catalyzing Pyrazolopyrimidine Formation in the Biosynthesis of Formycin A Open
Genome scanning of Streptomyces kaniharaensis, the producer of formycin A, reveals two sets of purA, purB, purC, and purH genes. The Pur enzymes catalyze pyrimidine assembly of purine nucleobases. To test whether enzymes encoded by the sec…
View article: Unusual carbohydrate biosynthesis : ǂb mechanistic studies of DesII and the biosynthesis of formycin A
Unusual carbohydrate biosynthesis : ǂb mechanistic studies of DesII and the biosynthesis of formycin A Open
Carbohydrates are essential biomolecules in all living organisms. Besides serving as energy storage and structural building blocks in primary metabolism, carbohydrates represent the building blocks for numerous bioactive natural products. …