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View article: An l-fucose-responsive transcription factor cross-regulates the expression of a diverse array of carbohydrate-active enzymes in Trichoderma reesei
An l-fucose-responsive transcription factor cross-regulates the expression of a diverse array of carbohydrate-active enzymes in Trichoderma reesei Open
l -Fucose is a universal capping component of biomolecules found throughout all domains of life. Although fungi are renowned for their role in biomass recycling, the mechanisms by which they process l -fucose remain largely unknown. In thi…
View article: Structure-guided engineering of transcriptional activator XYR1 for inducer-free production of lignocellulolytic enzymes in Trichoderma reesei
Structure-guided engineering of transcriptional activator XYR1 for inducer-free production of lignocellulolytic enzymes in Trichoderma reesei Open
View article: Production and Characterization of Cellulose Nanocrystals from Eucalyptus Dissolving Pulp Using Endoglucanases from Myceliophthora thermophila
Production and Characterization of Cellulose Nanocrystals from Eucalyptus Dissolving Pulp Using Endoglucanases from Myceliophthora thermophila Open
Endoglucanase (EG) is a key enzyme during enzymatic preparation of cellulose nanocrystals (CNCs). Myceliophthora thermophila is a thermophilic fungus that has thermal properties and a high secretion of endoglucanases (EGs), and could serve…
View article: Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei
Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei Open
View article: Production of cellulosic ethanol and value-added products from corn fiber
Production of cellulosic ethanol and value-added products from corn fiber Open
View article: A Detoxification-Free Process for Enhanced Ethanol Production From Corn Fiber Under Semi-Simultaneous Saccharification and Fermentation
A Detoxification-Free Process for Enhanced Ethanol Production From Corn Fiber Under Semi-Simultaneous Saccharification and Fermentation Open
Corn fiber, a by-product from the corn-processing industry, is an attractive feedstock for cellulosic ethanol because of its rich carbohydrate content (mainly residual starch, cellulose, and hemicellulose), abundant reserves, easy collecti…
View article: Pretreatment Affects Profits From Xylanase During Enzymatic Saccharification of Corn Stover Through Changing the Interaction Between Lignin and Xylanase Protein
Pretreatment Affects Profits From Xylanase During Enzymatic Saccharification of Corn Stover Through Changing the Interaction Between Lignin and Xylanase Protein Open
Effective pretreatment is vital to improve the biomass conversion efficiency, which often requires the addition of xylanase as an accessory enzyme to enhance enzymatic saccharification of corn stover. In this study, we investigated the eff…
View article: Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei
Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei Open
Background Cellulolytic enzyme production in filamentous fungi requires a release from carbon catabolite repression (CCR). The protein CRE1/CreA (CRE = catabolite responsive element) is a key transcription factor (TF) that is involved in C…
View article: Integrated engineering of enzymes and microorganisms for improving the efficiency of industrial lignocellulose deconstruction
Integrated engineering of enzymes and microorganisms for improving the efficiency of industrial lignocellulose deconstruction Open
View article: A Homeodomain-Containing Transcriptional Factor PoHtf1 Regulated the Development and Cellulase Expression in Penicillium oxalicum
A Homeodomain-Containing Transcriptional Factor PoHtf1 Regulated the Development and Cellulase Expression in Penicillium oxalicum Open
Homeodomain-containing transcription factors (Htfs) play important roles in animals, fungi, and plants during some developmental processes. Here, a homeodomain-containing transcription factor Po Htf1 was functionally characterized in the c…
View article: Efficient Constitutive Expression of Cellulolytic Enzymes in <i>Penicillium oxalicum</i> for Improved Efficiency of Lignocellulose Degradation
Efficient Constitutive Expression of Cellulolytic Enzymes in <i>Penicillium oxalicum</i> for Improved Efficiency of Lignocellulose Degradation Open
Efficient cellulolytic enzyme production is important for the development of lignocellulose-degrading enzyme mixtures. However, purification of cellulases from their native hosts is time- and labor-consuming. In this study, a constitutive …
View article: Disruption of the <i>Trichoderma reesei gul1</i> gene stimulates hyphal branching and reduces broth viscosity in cellulase production
Disruption of the <i>Trichoderma reesei gul1</i> gene stimulates hyphal branching and reduces broth viscosity in cellulase production Open
Hyphal morphology is considered to have a close relationship with the production level of secreted proteins by filamentous fungi. In this study, the gul1 gene, which encodes a putative mRNA-binding protein, was disrupted in cellulase-produ…
View article: Potassium permanganate assisted organosolv pretreatment enhances enzymatic hydrolysis of corn stover
Potassium permanganate assisted organosolv pretreatment enhances enzymatic hydrolysis of corn stover Open
High efficiency enzymatic hydrolysis of lignocellulose to produce fermentable sugar is important for bioconversion of lignocellulosic biomass to liquid fuels and chemicals. In this study, a simple pretreatment with potassium permanganate i…
View article: Additional file 3 of Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei
Additional file 3 of Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei Open
Additional file 3: Spreadsheet S1. Proteins interacting with T. reesei TrCRE1, P. oxalicum PoCreA, and PoCyc8 identified through TAP-MS. The peptide counts (PepCount) of each biological replicate, the sum of PepCount of three biological re…
View article: Enhancement of Cellulase Production in Trichoderma reesei via Disruption of Multiple Protease Genes Identified by Comparative Secretomics
Enhancement of Cellulase Production in Trichoderma reesei via Disruption of Multiple Protease Genes Identified by Comparative Secretomics Open
The filamentous fungus Trichoderma reesei is one of the most studied cellulolytic organisms and the major producer of cellulases for industrial applications. However, undesired degradation of cellulases often happens in culture filt…
View article: The Indispensable Role of Histone Methyltransferase PoDot1 in Extracellular Glycoside Hydrolase Biosynthesis of Penicillium oxalicum
The Indispensable Role of Histone Methyltransferase PoDot1 in Extracellular Glycoside Hydrolase Biosynthesis of Penicillium oxalicum Open
Histone methylation is associated with transcription regulation, but its role for glycoside hydrolase (GH) biosynthesis is still poorly understood. We identified the histone H3 lysine 79 (H3K79)-specific methyltransferase PoDot1 in …
View article: The GATA-Type Transcriptional Factor Are1 Modulates the Expression of Extracellular Proteases and Cellulases in Trichoderma reesei
The GATA-Type Transcriptional Factor Are1 Modulates the Expression of Extracellular Proteases and Cellulases in Trichoderma reesei Open
Trichoderma reesei is a biotechnologically important filamentous fungus with the remarkable ability to secrete large amounts of enzymes, whose production is strongly affected by both the carbon and nitrogen sources. While the carbon metabo…
View article: Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum
Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum Open
View article: MOESM3 of Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum
MOESM3 of Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum Open
Additional file 3: Spreadsheet S1. A total of 469 genes exhibited significant differences (fold change ≥ 2, probability ≥ 0.8) in ΔPoset2 compared with that of WT in the glucose medium.
View article: MOESM7 of Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum
MOESM7 of Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum Open
Additional file 7: Spreadsheet S3. Putative proteins interacting with PoSwd2 identified using TAP-MS experiments.
View article: MOESM4 of Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum
MOESM4 of Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum Open
Additional file 4: Spreadsheet S2. A total of 1457 genes exhibited significant differences (fold change ≥ 2, probability ≥ 0.8) in ΔPoset2 compared with that of WT in cellulose medium.
View article: Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production
Cow manure as a lignocellulosic substrate for fungal cellulase expression and bioethanol production Open
View article: Production of highly efficient cellulase mixtures by genetically exploiting the potentials of Trichoderma reesei endogenous cellulases for hydrolysis of corncob residues
Production of highly efficient cellulase mixtures by genetically exploiting the potentials of Trichoderma reesei endogenous cellulases for hydrolysis of corncob residues Open
View article: Production of the versatile cellulase for cellulose bioconversion and cellulase inducer synthesis by genetic improvement of Trichoderma reesei
Production of the versatile cellulase for cellulose bioconversion and cellulase inducer synthesis by genetic improvement of Trichoderma reesei Open
View article: Combining manipulation of transcription factors and overexpression of the target genes to enhance lignocellulolytic enzyme production in Penicillium oxalicum
Combining manipulation of transcription factors and overexpression of the target genes to enhance lignocellulolytic enzyme production in Penicillium oxalicum Open
View article: MOESM8 of Combining manipulation of transcription factors and overexpression of the target genes to enhance lignocellulolytic enzyme production in Penicillium oxalicum
MOESM8 of Combining manipulation of transcription factors and overexpression of the target genes to enhance lignocellulolytic enzyme production in Penicillium oxalicum Open
Additional file 8. Table S1 Primers used in this study.
View article: The Different Roles of Penicillium oxalicum LaeA in the Production of Extracellular Cellulase and β-xylosidase
The Different Roles of Penicillium oxalicum LaeA in the Production of Extracellular Cellulase and β-xylosidase Open
Cellulolytic enzyme hydrolysis of lignocellulose biomass to release fermentable sugars is one of the key steps in biofuel refining. Gene expression of fungal cellulolytic enzymes is tightly controlled at the transcriptional level. Key tran…
View article: Expression and chromatin structures of cellulolytic enzyme gene regulated by heterochromatin protein 1
Expression and chromatin structures of cellulolytic enzyme gene regulated by heterochromatin protein 1 Open
HepA is required for chromatin condensation of prominent cellulase genes. However, the opening of chromatin mediated by the deletion of hepA was not positively correlated with cellulolytic enzyme gene activation. HepA is actually a …
View article: Characterization and Strain Improvement of a Hypercellulytic Variant, Trichoderma reesei SN1, by Genetic Engineering for Optimized Cellulase Production in Biomass Conversion Improvement
Characterization and Strain Improvement of a Hypercellulytic Variant, Trichoderma reesei SN1, by Genetic Engineering for Optimized Cellulase Production in Biomass Conversion Improvement Open
The filamentous fungus Trichoderma reesei is a widely used strain for cellulolytic enzyme production. A hypercellulolytic T. reesei variant SN1 was identified in this study and found to be different from the well-known cellulase producers …
View article: Production of a high-efficiency cellulase complex via β-glucosidase engineering in Penicillium oxalicum
Production of a high-efficiency cellulase complex via β-glucosidase engineering in Penicillium oxalicum Open