Yo Ueki
YOU?
Author Swipe
View article: Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion–Insertion Mutation in S-Protein Gene
Rapid Detection of SARS-CoV-2 RNA Using Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) with Lateral Flow for N-Protein Gene and Variant-Specific Deletion–Insertion Mutation in S-Protein Gene Open
Rapid molecular testing for severe acute respiratory coronavirus 2 (SARS-CoV-2) variants may contribute to the development of public health measures, particularly in resource-limited areas. Reverse transcription recombinase polymerase ampl…
Distribution of Human Sapovirus Strain Genotypes over the Last Four Decades in Japan: a Global Perspective Open
Sapovirus (SaV) infections are a public health problem because they cause acute gastroenteritis in humans of all ages, both sporadically and as outbreaks. However, only a limited amount of SaV sequence information, especially whole-genome …
Characteristics of Norovirus GII Accumulation in Oysters: Comparison of GII. 2 and GII. 4 Open
牡蠣を人為的にノロウイルスGII.2とGII.4に汚染する実験を行い,牡蠣に蓄積されたノロウイルス量の分析を行った.ノロウイルスGII.2(陽性率:80.0%)はGII.4(陽性率:60.0%)よりも牡蠣に蓄積されやすい傾向があった.遺伝子型に関わらず,高濃度のノロウイルスGIIを添加した人工海水で24時間飼育しても,ノロウイルスGIIを蓄積しない牡蠣が存在し,ウイルス蓄積量の多い牡蠣(中腸腺1g当たり約4.08log copies)と少ない牡蠣では100倍以上の個体差があ…
Genetic analysis of sapoviruses detected in outbreaks and sporadic cases of acute gastroenteritis in Miyagi Prefecture, Japan Open
The distribution of SaV genotypes was different between foodborne outbreaks and other settings. The effective SaV infection control may differ depending on the genomic characteristics.
Comparison of RNA Extraction Methods of Norovirus Evaluated by Real-Time PCR Open
RNA extraction is essential for reverse transcription (RT) PCR or RT real-time PCR to detect many viral pathogens in various samples. Owing to the lack of cell culture methods of norovirus, only molecular methods have been applied to the d…
Detection and Molecular Analysis of Hepatitis E Virus for Wild Boars, Wild Deer, Pigs and Sewage in Miyagi Open
We investigated the prevalence of Hepatitis E virus (HEV) using RT-PCR among wild boars, wild deer and pigs in Miyagi prefecture from 2015 to 2017. We conducted to detect of HEV gene from sewage from 2016 to 2018. Samples of livers were co…
Evolutionary Analysis of the VP1 and RNA-Dependent RNA Polymerase Regions of Human Norovirus GII.P17-GII.17 in 2013–2017 Open
Human norovirus (HuNoV) GII.P17-GII.17 (Kawasaki2014 variant) reportedly emerged in 2014 and caused gastroenteritis outbreaks worldwide. To clarify the evolution of both VP1 and RNA-dependent RNA polymerase (RdRp) regions of GII.P17-GII.17…
Isolation of Extended Spectrum β-lactamase-producing <i>Escherichia coli</i> at Poultry Processing Plant in Miyagi, Japan Open
宮城県内の大規模食鳥処理場における基質特異性拡張型β-ラクタマーゼ（ESBL）産生大腸菌の浸潤状況を調査し，処理工程における交差汚染の有無について検討した．ブロイラーと体120検体中78検体（65％）からESBL産生大腸菌が分離された．検出されたESBL遺伝子はbla CTX-M-1 group及びbla CTX-M-2 groupであり，CTX-M型が主であった．また，O抗原血清型はO18，O111，O153，O157及び血清型不明であった．これらの結果はロットによって異…
Genetic Analysis of Human Norovirus Strains in Japan in 2016–2017 Open
In the 2016/2017 winter season in Japan, HuNoV GII.P16-GII.2 strains (2016 strains) emerged and caused large outbreaks of acute gastroenteritis. To better understand the outbreaks, we examined the molecular evolution of the VP1 gene and Rd…
Phylogeny and Immunoreactivity of Norovirus GII.P16-GII.2, Japan, Winter 2016–17 Open
During the 2016-17 winter season in Japan, human norovirus GII.P16-GII.2 strains (2016 strains) caused large outbreaks of acute gastroenteritis. Phylogenetic analyses suggested that the 2016 strains derived from the GII.2 strains detected …
Molecular Evolution of the RNA-Dependent RNA Polymerase and Capsid Genes of Human Norovirus Genotype GII.2 in Japan during 2004–2015 Open
The RNA-dependent RNA polymerase (RdRp) and capsid (VP1) genes of 51 GII.2 human norovirus (HuNoV) strains collected during the period of 2004-2015 in Japan were analyzed. Full-length analyses of the genes were performed using next-generat…
Comparison of Methods to Recover Norovirus from Oysters Open
To keep the safety of oyster intended for raw eating, monitoring of norovirus (NoV) contamination by rapid and sensitive method is important. We had developed a rapid method using bead homogenization to recover NoV particles from oyster ti…
Effectiveness of a Nested Real-Time PCR Assay for Detection of Norovirus in Oysters Open
宮城県では，生カキ喫食に関連したノロウイルス(NoV)による食中毒を未然に防止するため，市販カキを対象としてNoV遺伝子検出検査を実施している．検査は，厚生労働省の通知（食安監発第1105001号「III リアルタイムPCR法によるノロウイルスの定量的検出法」，以下通知法）に従って行われていた．しかし，過去にわれわれが行った調査では，real-time PCR法により検出されたNoV遺伝子数が陽性基準値未満であっても増幅曲線が確認されたすべての検体から，nested PCR…